FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20806355
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT20645339.92260778017101No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT9096384.371923866530202No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6908253.320259603375988No Hit
TTCAAGTAATCCAGGATAGGCT3723381.7895397824366641No Hit
TATTGCACTTGTCCCGGCCTGT3674021.765816261425896No Hit
AAACCGTTACCATTACTGAGC3641291.750085490707046No Hit
TCCTGTACTGAGCTGCCCCGAG2602491.25081495533456No Hit
TCCTGTACTGAGCTGCCCCGA2596861.2481090512970676No Hit
TGAGGTAGTAGGTTGTATAGTT2096001.0073845226614657No Hit
TGAGGTAGTAGGTTGTATAGT2082781.0010306947084195No Hit
TAGCTTATCAGACTGATGTTGAC1973600.9485563425213114No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1971750.9476671911057943No Hit
CGCGACCTCAGATCAGA1964520.9441922912494763No Hit
TCAGTGCACTACAGAACTTTGT1774360.8527971381820602No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1765330.8484571180295636No Hit
TGAGGTAGTAGATTGTATAGTT1731900.8323899116399772No Hit
TGGAGTGTGACAATGGTGTTT1672590.8038841978808879No Hit
TGAGGTAGTAGATTGTATAGT1375500.6610960929965869No Hit
AAGCTGCCAGTTGAAGAACTGT1163050.5589878669281573No Hit
TGAGGGGCAGAGAGCGAGACTTT1149200.5523312468714486No Hit
TGAGGTAGTAGGTTGTGTGGTT1147730.5516247319629026No Hit
TGAGGTAGTAGGTTGTGTGGT1146840.5511969780386811No Hit
TGAGGTAGTAGTTTGTGCTGTT1123440.5399504141883573No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1058950.5089550764658202No Hit
TCCTGTACTGAGCTGCCCCGAGA1047860.5036249741965857No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1027940.4940509762522076No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT954910.458951123346689No Hit
TGTAAACATCCCCGACTGGAAGCT953110.4580860030505103No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT927380.445719589039022No Hit
TCCTGTACTGAGCTGCCCCGAGT896510.43088277595955654No Hit
TCGTACCGTGAGTAATAATGCG837420.4024827991255557No Hit
TGAGGTAGTAGTTTGTACAGT826330.3971526968563211No Hit
TATTGCACTTGTCCCGGCCTGC824670.39635486369428957No Hit
TTCAAGTAATCCAGGATAGGCC809540.3890830469825205No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT798800.383921162548654No Hit
TAGCTTATCAGACTGATGTTGA790830.3800906021261292No Hit
TGAGGGGCAGAGAGCGAGACT763050.3667389122217707No Hit
AAACCGTTACCATTACTGAG733710.3526374513940572No Hit
TCCTGTACTGAGCTGCCCCG700240.33655102010900034No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA686990.3301827734843513No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT652950.3138223874388378No Hit
CCCATAAAGTAGAAAGCACT532250.25581126535618565No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT526530.2530621053038843No Hit
CATTGCACTTGTCTCGGTCTGA525500.25256706424551534No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC516250.24812130716793018No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC474100.2278630735657447No Hit
TGAGGTAGTAGGTTGTATAG474050.2278390424464064No Hit
TGAGAACTGAATTCCATGGGTT471000.2263731441667702No Hit
TTCAAGTAATTCAGGATAGGT453470.21794783372676282No Hit
AAAAGCTGGGTTGAGAGGGCGA441830.21235338914480698No Hit
CGCGACCTCAGATCAGACGT439960.2114546252815546No Hit
TAGCTTATCAGACTGATGTTGAT425190.20435583262902127No Hit
TGAGGTAGTAGTTTGTACAGTT424320.2039376911525349No Hit
TAGCTTATCAGACTGATGTTG422530.2030773770802238No Hit
TATTGCACTTGTCCCGGCCTG416050.19996294401398032No Hit
TGAGGTAGTAGGTTGTATAGTC397270.1909368555905155No Hit
TGAGGTAGTAGGTTGTATAGC371150.17838299884818845No Hit
AGCTCGGTCTGAGGCCCCTCAGT366740.17626345412255054No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG359130.17260591775926154No Hit
AAACCGTTACCATTACTG358770.1724328937000258No Hit
TGTAAACATCCTTGACTGGAAGCT347680.1671027914307912No Hit
AAACCGTTACCATTACTGAGA336740.16184478251957155No Hit
TCAGTGCACTACAGAACTTTGC332250.15968678800299235No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT325750.15656274248901356No Hit
TGAGGTAGTAGATTGTATAGTC318850.1532464480203284No Hit
TCCTGTACTGAGCTGCCCCGAA315850.15180458086003049No Hit
TGTAAACATCCCCGACTGGAAGC310930.14943991871714193No Hit
CGCGACCTCAGATC306100.14711851258906233No Hit
TGGCTCAGTTCAGCAGGAAC294510.14154809912644478No Hit
TTCAAGTAATCCAGGATAGGCA293330.14098096471006094No Hit
CAACGGAATCCCAAAAGCAGCT291140.1399284016830435No Hit
CTAGACTGAAGCTCCTTGAGG287340.1381020366133328No Hit
TGAGGTAGTAGGTTGTGTGG286960.13791940010636172No Hit
TGGAGTGTGACAATGGTGTTC279420.13429550731014636No Hit
TGAGGGGCAGAGAGCGAGACTT274240.13180588334669863No Hit
TGAGGTAGTAGTTTGTGCTGT268770.1291768788910888No Hit
AAACCGTTACCATTACTGAGTT267470.12855206978829306No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT263090.12644694373425813No Hit
AGAGGTAGTAGGTTGCATAGTT251570.12091017383871418No Hit
TGAGGTAGTAGATTGTATAG243270.11692100802855665No Hit
GGCTGGTCCGATGGTAGTGGGTT243010.1167960462079975No Hit
TAGCTTATCAGACTGATGTTGACT241370.11600782549370132No Hit
CGCGACCTCAGATCAGAC237700.11424394133427021No Hit
TGAGGTAGTAGGTTGTAT236950.11388347454419576No Hit
TGAGGTAGTAGATTGTATAGC236700.11376331894750426No Hit
TGAGGTAGTAGGTTGTGTGGTC231530.11127850120792422No Hit
TATTGCACTTGTCCCGGCCTGTA229770.11043260580721612No Hit
GCATTGGTGGTTCAGTGGTAGAATT229760.11042779958334846No Hit
TGAGGTAGTAGGTTGTGTGGC227660.10941849257113992No Hit
ACCAATATTACTGTGCTGCTT226030.10863507808071139No Hit
AAGCTGCCAGTTGAAGAACTGC225510.1083851544395931No Hit
TGGCTCAGTTCAGCAGGAACAG221270.10634731551970539No Hit
CAACGGAATCCCAAAAGCAGCTG220610.10603010474443984No Hit
AAGCTGCCAGTTGAAGAACT220330.10589553047614537No Hit
TGTAAACATCCCCGACTGGAAGCC219630.10555909480540922No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA216930.1042614143611411No Hit
CATTGCACTTGTCTCGGTCTG214650.10316559531931471No Hit
GGCTGGTCCGATGGTAGTGGGT213970.10283877209631383No Hit
TGTAAACATCCCCGACTGGAAG213210.10247349908237172No Hit
TGAGGTAGTAGTTTGTGCTGTC213170.10245427418690108No Hit
AACCCGTAGATCCGATCTTGT213000.10237256838115086No Hit
TGAGGTAGTAGATTGTAT212360.10206497005362063No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCC210070.10096434478792657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)