FastQCFastQC Report
Fri 10 May 2024
EGAF00002167566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18805272
Total Bases454 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9732685.175506102756716No Hit
TCAGTGCACTACAGAACTTTGT5900343.137598860574843No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4812252.5589898407212615No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC3886382.066643864550324No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2922901.554298177659967No Hit
TATTGCACTTGTCCCGGCCTGT2535971.3485420471450773No Hit
TTCAAGTAATCCAGGATAGGCT2325351.2365415400532362No Hit
CGCGACCTCAGATCAGA2116061.1252482814393752No Hit
AAACCGTTACCATTACTGAGC1724710.917141746208191No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1549960.8242156773908934No Hit
TCCTGTACTGAGCTGCCCCGAG1522870.8098101426025638No Hit
AAGCTGCCAGTTGAAGAACTGT1514830.8055347457883086No Hit
TAGCTTATCAGACTGATGTTGAC1472620.7830889125134697No Hit
TCCTGTACTGAGCTGCCCCGA1401330.7451793305621955No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1346260.7158949894476401No Hit
TGAGGGGCAGAGAGCGAGACTTT1314630.699075238050266No Hit
TGAGGTAGTAGGTTGTATAGTT1179510.6272230468136807No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1170900.6226445435088628No Hit
TGGAGTGTGACAATGGTGTTT981200.5217685763864517No Hit
TAGCTTATCAGACTGATGTTGA965260.51329222996615No Hit
TGAGGTAGTAGGTTGTATAGT938510.4990674955406122No Hit
TGAGGTAGTAGATTGTATAGTT886070.4711816984088292No Hit
TCAGTGCACTACAGAACTTTGC864230.45956793392831546No Hit
CCCATAAAGTAGAAAGCACT846460.45011845614357504No Hit
CATTGCACTTGTCTCGGTCTGA826120.43930234032243726No Hit
TGAGGGGCAGAGAGCGAGACT720690.38323827488376666No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC719730.382727779741766No Hit
TCGTACCGTGAGTAATAATGCG676520.3597501806940096No Hit
TTCACAGTGGCTAAGTTCCG667010.35469308819356615No Hit
TGGCTCAGTTCAGCAGGAAC634620.3374691948087749No Hit
CGCGACCTCAGATCAGACGT627200.33352349277372856No Hit
CGCGACCTCAGATC614360.32669562024947046No Hit
TGAGGTAGTAGATTGTATAGT611050.32493547554111424No Hit
TGAGGTAGTAGGTTGTGTGGTT575400.3059780257366126No Hit
TAGCTTATCAGACTGATGTTG531890.282840896956981No Hit
TGAGGTAGTAGTTTGTGCTGTT531610.28269200254056415No Hit
TATTGCACTTGTCCCGGCCTGC477620.25398196846075927No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG465440.24750506134662664No Hit
AAACCGTTACCATTACTGAG461130.24521315086535309No Hit
TGAGGTAGTAGGTTGTGTGGT446590.23748127652713558No Hit
TGTAAACATCCCCGACTGGAAGCT440630.23431195252054848No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC432600.2300418733640226No Hit
AAAAGCTGGGTTGAGAGGGCGA418440.22251207001951367No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT416390.2214219501850332No Hit
GCATTGGTGGTTCAGTGGTAGAATT412670.21944378150978086No Hit
TTCAAGTAATCCAGGATAGGCC412290.2192417105160723No Hit
TCCTGTACTGAGCTGCCCCGAGA409540.2177793546405497No Hit
TCCTGTACTGAGCTGCCCCG409160.21757728364684117No Hit
TCCTGTACTGAGCTGCCCCGAGT407180.2165243874164649No Hit
TGAGGTAGTAGTTTGTACAGT405110.2154236322665261No Hit
TGGCTCAGTTCAGCAGGAACAG380050.20209758199721864No Hit
TGTAAACATCCCCGACTGGAAG379210.2016508987479681No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC369430.19645022948883697No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT362270.19264278655474912No Hit
TGAGATGAAGCACTGTAGCT361780.19238222132601962No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA329530.1752327751494368No Hit
TGTAAACATCCTTGACTGGAAGCT319470.1698832114738888No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG310050.1648739778930079No Hit
TGAGAACTGAATTCCATGGGTT305820.1626246086735677No Hit
TGAGGGGCAGAGAGCGAGACTT305010.1621938783975047No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT297570.158237541047No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT293900.15628596066039352No Hit
AGCTCGGTCTGAGGCCCCTCAGT282200.15006430111726116No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC274850.14615582268631902No Hit
CATTGCACTTGTCTCGGTCTG271580.14441694860887946No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT267540.14226861488629358No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT261940.13929072655795674No Hit
TGAGGTAGTAGGTTGTGTGG254280.13521740073741023No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA252310.13416982216476317No Hit
CTAGACTGAAGCTCCTTGAGG248930.1323724538523027No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT240620.12795348027935996No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC239580.127400443875526No Hit
AAGCTGCCAGTTGAAGAACTGC234000.12443319086264747No Hit
TGAAAAGAACTTTGAAGAGAG233730.12428961410395978No Hit
TGAGGTAGTAGTTTGTACAGTT229680.12213596272364473No Hit
TGAGATGAAGCACTGTAGCTC227810.12114156072828938No Hit
GGCTGGTCCGATGGTAGTGGGTT222570.11835510807820275No Hit
GGCTGGTCCGATGGTAGTGGGT221570.11782334230528546No Hit
TGAGGTAGTAGGTTGTATAG218190.11602597399282498No Hit
TCAGTGCATCACAGAACTTTGT215390.11453702982865656No Hit
CGTGTATTTGACAAGCTGAGTTG209510.11141024708390286No Hit
GGCTGGTCCGATGGTAGT209260.11127730564067352No Hit
TATTGCACTTGTCCCGGCCTG208040.11062855139771444No Hit
AAACCGTTACCATTACTG208030.11062323373998525No Hit
TGTAAACATCCCCGACTGGAAGC207230.11019782112165141No Hit
AAGCTGCCAGTTGAAGAACT207170.11016591517527638No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA200810.10678388485952238No Hit
TTCAAGTAATTCAGGATAGGT198210.10540129384993739No Hit
CAACGGAATCCCAAAAGCAGCTG195900.10417291491449845No Hit
AAATGGATTTTTGGAGCAGGGAGA194880.10363051382612279No Hit
AACCCGTAGATCCGATCTTGT193580.10293921832133031No Hit
TGGCTCAGTTCAGCAGGAACA193230.10275310030080927No Hit
CGCGACCTCAGATCAGAC190590.1013492386603076No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA189470.10075366099464023No Hit
AAACCGTTACCATTACTGAGTT188610.10029634242993135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)