FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12339102
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT140917611.420409686215415No Hit
CGCGACCTCAGATCAGA3839953.1120173899202714No Hit
TGAGGTAGTAGGTTGTATAGT3184582.5808847353721527No Hit
TGAGGTAGTAGGTTGTATAGTT2145951.7391460091666315No Hit
AAACCGTTACCATTACTGAGC2145511.7387894191976043No Hit
TCAGTGCACTACAGAACTTTGT2126071.7230346260205969No Hit
TCCTGTACTGAGCTGCCCCGAG1971421.5977013562251126No Hit
TCCTGTACTGAGCTGCCCCGA1825511.4794512599052994No Hit
TGAGGTAGTAGATTGTATAGT1671681.3547825441430017No Hit
TGAGGTAGTAGGTTGTGTGGT1465221.1874608054945976No Hit
TGGAGTGTGACAATGGTGTTT1408901.141817289459152No Hit
TTCAAGTAATCCAGGATAGGCT1322021.0714069792112912No Hit
TGAGGTAGTAGATTGTATAGTT1241451.0061104932919753No Hit
TATTGCACTTGTCCCGGCCTGT1203070.9750061228118546No Hit
TGAGGTAGTAGGTTGTGTGGTT1161470.9412921621038548No Hit
TGAGGGGCAGAGAGCGAGACT874420.7086577289011794No Hit
TGAGGGGCAGAGAGCGAGACTTT873180.7076527935339216No Hit
TGAGGTAGTAGTTTGTGCTGTT719960.5834784411377749No Hit
TGAGGTAGTAGTTTGTACAGT686920.5567017761908444No Hit
TGAGGTAGTAGGTTGTATAG659800.5347228671908215No Hit
AAGCTGCCAGTTGAAGAACTGT654040.53005478032356No Hit
TCCTGTACTGAGCTGCCCCGAGA583380.4727896730248279No Hit
AAACCGTTACCATTACTGAG556940.45136185761330117No Hit
TCCTGTACTGAGCTGCCCCG527670.4276405203555332No Hit
TGAGGTAGTAGGTTGTATAGC494790.4009935244882489No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC494680.4009043769959921No Hit
CCCATAAAGTAGAAAGCACT466650.37818797510548174No Hit
TAGCTTATCAGACTGATGTTGAC449330.36415129723378575No Hit
TCCTGTACTGAGCTGCCCCGAGT449120.36398110656675015No Hit
TCGTACCGTGAGTAATAATGCG432830.35077917339527626No Hit
CGCGACCTCAGATCAGACGT427030.34607866925810327No Hit
AAAAGCTGGGTTGAGAGGGCGA426420.34558430589195227No Hit
TGAGGTAGTAGGTTGTGTGG390480.31645738887643526No Hit
TTCAAGTAATTCAGGATAGGT387450.3140017806806362No Hit
TGAGGTAGTAGGTTGTATAGTC353420.2864227883033952No Hit
TCAGTGCACTACAGAACTTTGC345220.27977724797152986No Hit
CATTGCACTTGTCTCGGTCTGA344500.2791937371131222No Hit
TGAGGTAGTAGTTTGTACAGTT330630.26795304877129633No Hit
TGGCTCAGTTCAGCAGGAAC329130.26673740114961364No Hit
TGAGGTAGTAGGTTGTAT317090.2569798029062406No Hit
TAGCTTATCAGACTGATGTTGA311480.25243328080114746No Hit
AGAGGTAGTAGGTTGCATAGT309480.2508124173055705No Hit
AAACCGTTACCATTACTG277460.22486239274138428No Hit
TGAGGTAGTAGATTGTATAG271360.21991875907987468No Hit
CGCGACCTCAGATCAGAC266390.21589091329336607No Hit
TGAGGGGCAGAGAGCGAGACTT264530.21438351024247954No Hit
TGAGGTAGTAGGTTGTGTGGC260270.21093107099690073No Hit
TTCAAGTAATCCAGGATAGGCC257210.20845114984866805No Hit
TGAGGTAGTAGATTGTATAGC255610.20715445905220656No Hit
GGTGGGCGTAGCTC254250.20605227187521424No Hit
CGCGACCTCAGATC249840.20247826786746717No Hit
TTCACAGTGGCTAAGTTCCG248430.20133555910308545No Hit
TAGCTTATCAGACTGATGTTG240570.19496556556546824No Hit
TATTGCACTTGTCCCGGCCTGC236660.19179677743161536No Hit
AAATGGATTTTTGGAGCAGGGAGA227980.1847622298608116No Hit
AGAGGTAGTAGGTTGCATAGTT221620.17960788394487703No Hit
TCCTGTACTGAGCTGCCCCGAA218100.17675516419266166No Hit
TGGAGTGTGACAATGGTGTTC208720.16915331439840597No Hit
TGAGGTAGTAGATTGTAT207640.16827804811079444No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT206930.16770264156986464No Hit
TGAGGTAGTAGGTTGTGTGGTC204520.16574950105769445No Hit
AAACCGTTACCATTACTGAGA202230.1638936123552589No Hit
TGAGGTAGTAGATTGTATAGTC199190.16142989984198203No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT198860.16116245736521184No Hit
AAGCTGCCAGTTGAAGAACT198340.16074103285636182No Hit
CAACGGAATCCCAAAAGCAGCT196080.15890945710635992No Hit
TGTAAACATCCCCGACTGGAAGCT187270.15176955340834364No Hit
TGGAGAGAAAGGCAGTTCCTGA181270.14690696292161295No Hit
TGAGGTAGTAGTTTGTGCTGT181070.14674487657205523No Hit
TATTGCACTTGTCCCGGCCTG169510.13737628556762072No Hit
TGAGAACTGAATTCCATGGGTT161560.13093335317270252No Hit
AAACCGTTACCATTACTGAGTT160810.13032552936186118No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG159760.1294745760266833No Hit
CTAGACTGAAGCTCCTTGAGG156040.12645976992491026No Hit
TGAGGGGCAGAGAGCGAGACC152120.12328287747357951No Hit
TGAGGTAGTAGTTTGTGCT151220.1225534889005699No Hit
GGCTGGTCCGATGGTAGT150240.12175926578773724No Hit
TACAGTATAGATGATGTACT144550.11714790914282092No Hit
CATTGCACTTGTCTCGGTCTG143990.1166940673640594No Hit
AAATGGATTTTTGGAGCAGGGAG143590.11636989466494402No Hit
AAAAGCTGGGTTGAGAGGGCG143040.11592415720366037No Hit
AAATGGATTTTTGGAGCAGGGA138820.11250413522799309No Hit
ATTTTTGGAGCAGGGAGA138390.11215564957644404No Hit
GGCTGGTCCGATGGTAGTGGGT136420.11055909903330079No Hit
AGCTCGGTCTGAGGCCCCTCAGT135670.10995127522245947No Hit
AAAAGCTGGGTTGAGAGGGCGT130820.10602068124568545No Hit
TGGCTCAGTTCAGCAGGAACAG129460.10491849406869316No Hit
AACCCGTAGATCCGATCTTGT125490.10170108002997302No Hit
TTTTTGGAGCAGGGAGA124260.10070424898019321No Hit
AAACCGTTACTATTACTGAGT123580.10015315539169706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)