FastQCFastQC Report
Wed 8 May 2024
EGAF00002167571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9652300
Total Bases202.5 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT98234610.177325611512282No Hit
TCAGTGCACTACAGAACTTTGT2745502.8443997803632293No Hit
CGCGACCTCAGATCAGA2603312.697087740745729No Hit
TGAGGTAGTAGGTTGTATAGT1771511.835324223242129No Hit
AAACCGTTACCATTACTGAGC1658961.7187198905960237No Hit
TTCAAGTAATCCAGGATAGGCT1385081.4349740476363146No Hit
TGAGGTAGTAGGTTGTATAGTT1273011.3188670057913658No Hit
TGAGGTAGTAGATTGTATAGT1082461.121452917957378No Hit
TGGAGTGTGACAATGGTGTTT1063371.101675248386395No Hit
TCCTGTACTGAGCTGCCCCGA1011261.0476881157858748No Hit
TCCTGTACTGAGCTGCCCCGAG979971.015270971685505No Hit
TGAGGTAGTAGGTTGTGTGGT873930.905411145530081No Hit
TGAGGTAGTAGATTGTATAGTT871010.9023859598230475No Hit
TATTGCACTTGTCCCGGCCTGT754500.7816789780674037No Hit
TGAGGGGCAGAGAGCGAGACT716050.7418439128497871No Hit
AAGCTGCCAGTTGAAGAACTGT688440.7132393315582815No Hit
TGAGGTAGTAGGTTGTGTGGTT653590.6771339473493365No Hit
TGAGGGGCAGAGAGCGAGACTTT628350.6509847393885395No Hit
TAGCTTATCAGACTGATGTTGAC583810.6048402971312536No Hit
TGAGGTAGTAGTTTGTGCTGTT562400.5826590553546823No Hit
CCCATAAAGTAGAAAGCACT509910.5282782342032469No Hit
TCAGTGCACTACAGAACTTTGC498990.5169648684769432No Hit
TCGTACCGTGAGTAATAATGCG490780.5084591237321675No Hit
TGAGGTAGTAGTTTGTACAGT476220.493374636097096No Hit
TAGCTTATCAGACTGATGTTGA443410.4593827377930649No Hit
CGCGACCTCAGATCAGACGT426830.44220548470312776No Hit
TGGCTCAGTTCAGCAGGAAC424130.4394082239466241No Hit
TGAGGTAGTAGGTTGTATAG379810.3934917066398682No Hit
AAACCGTTACCATTACTGAG375620.3891507723547755No Hit
TTCACAGTGGCTAAGTTCCG366110.3792981983568683No Hit
TCCTGTACTGAGCTGCCCCG357810.37069921158687563No Hit
TGACTAGAACATTCACGCTTT355050.3678397894802275No Hit
TGAGGTAGTAGGTTGTGTGG349080.3616547351408472No Hit
AAAAGCTGGGTTGAGAGGGCGA309430.3205764429203402No Hit
TGAGGTAGTAGGTTGTATAGC308460.31957150109300375No Hit
CATTGCACTTGTCTCGGTCTGA304300.31526164748298335No Hit
TTCAAGTAATCCAGGATAGGCC291460.30195911855205493No Hit
TAGCTTATCAGACTGATGTTG282440.292614195580328No Hit
CTAGACTGAAGCTCCTTGAGG279390.2894543269479813No Hit
TCCTGTACTGAGCTGCCCCGAGA250720.2597515618039224No Hit
AAACCGTTACCATTACTG248800.2577623985992976No Hit
TTCAAGTAATTCAGGATAGGT247340.25624980574578077No Hit
TGAGGTAGTAGGTTGTATAGTC238690.2472882110999451No Hit
TGAGGTAGTAGGTTGTAT236610.2451332842949349No Hit
CGCGACCTCAGATC233370.24177657138713055No Hit
TCCTGTACTGAGCTGCCCCGAGT216800.2246096785222175No Hit
TGAGATGAAGCACTGTAGCT214360.22208178361634015No Hit
TGAGGTAGTAGTTTGTACAGTT205020.21240533344384238No Hit
AAATGGATTTTTGGAGCAGGGA196160.20322617407250088No Hit
TGAGGTAGTAGATTGTAT189060.1958704143053987No Hit
TGAGGTAGTAGATTGTATAGC188140.19491727360318264No Hit
TGAGAACTGAATTCCATGGGTT187680.19444070325207463No Hit
TGAGGTAGTAGATTGTATAG187630.1943889021269542No Hit
TGAGGGGCAGAGAGCGAGACTT181300.1878308796867068No Hit
TGGAGTGTGACAATGGTGTTC180120.18660837313386447No Hit
TGAGGTAGTAGGTTGTGTGGC170750.17690084228629446No Hit
AAGCTGCCAGTTGAAGAACT170540.1766832775607886No Hit
TGAGGTAGTAGTTTGTGCTGT165560.17152388549879302No Hit
TATTGCACTTGTCCCGGCCTGC163890.1697937279197704No Hit
AAATGGATTTTTGGAGCAGGGAGA162380.1682293339411332No Hit
TGAGGTAGTAGATTGTATAGTC159170.1649037017084011No Hit
AAATGGATTTTTGGAGCAGGG158600.16431316888202813No Hit
CGCGACCTCAGATCAGAC154720.16029340157268215No Hit
TGGCTCAGTTCAGCAGGAACAG152670.15816955544274422No Hit
TACAGTATAGATGATGTACT152590.15808667364255152No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC152480.15797271116728656No Hit
AAATGGATTTTTGGAGCAGGGAG150190.1556002196367705No Hit
AAACCGTTACCATTACTGAGA148210.1535488950820012No Hit
AGAGGTAGTAGGTTGCATAGT145920.15117640355148512No Hit
TGTAAACATCCCCGACTGGAAGCT142930.14807869626928297No Hit
TGAGGGGCAGAGAGCGAGACC138620.14361343928390125No Hit
TGAGATGAAGCACTGTAGCTC136720.1416449965293246No Hit
CATTGCACTTGTCTCGGTCTG134550.13939682769909761No Hit
TGAGGTAGTAGGTTGTGTGGTC130520.13522165701439035No Hit
TTCAAGTAATCCAGGATAGGCA129600.1342685163121743No Hit
TGAGGTAGTAGTTTGTGCT129580.13424779586212612No Hit
AAGCTGCCAGTTGAAGAACTGC129250.13390590843633124No Hit
AAAAGCTGGGTTGAGAGGGCG127270.1318545838815619No Hit
AGAGGTAGTAGGTTGCATAGTT126410.13096360452949038No Hit
TGTAAACATCCCCGACTGGAAG123350.1277933756721196No Hit
GCGGGCGTAGCTCAGGGGTAGAGC119250.12354568341224371No Hit
AACCCGTAGATCCGATCTTGT118030.12228173595930504No Hit
TCGTACCGTGAGTAATAATGCA116170.12035473410482475No Hit
ATTTTTGGAGCAGGGAGA111410.11542326699335909No Hit
TGAGGTAGTAGTTTGTGCTGTC108190.11208727453560291No Hit
TCCTGTACTGAGCTGCCCCGAA108170.11206655408555474No Hit
AAAAGCTGGGTTGAGAGGGCGT107810.1116935859846876No Hit
TTTTTGGAGCAGGGAGA107440.11131025765879635No Hit
GGCTGGTCCGATGGTAGT106140.10996342840566496No Hit
CAACGGAATCCCAAAAGCAGCT105980.10979766480527957No Hit
AGCTCGGTCTGAGGCCCCTCAGT103140.10685536089843871No Hit
TGGAGAGAAAGGCAGTTCCTGA101980.10565357479564456No Hit
TGGCTCAGTTCAGCAGGAACA101710.10537384871999418No Hit
TCAGTGCATCACAGAACTTTGT101430.10508376241931976No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT100810.10444142846782632No Hit
ACCAATATTACTGTGCTGCTT100090.10369549226609202No Hit
TACAGTACTGTGATAACTGAAG99430.10301171741450225No Hit
TAGCTTATCAGACTGATGTTGAT98660.1022139800876475No Hit
TATTGCACTTGTCCCGGCCTG97730.10125047916040737No Hit
TTTGGAGCAGGGAGA97650.10116759736021466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)