FastQCFastQC Report
Wed 8 May 2024
EGAF00002167572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15491278
Total Bases369.5 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10073946.502975416230991No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC7772995.0176557415082215No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT5423693.50112495560405No Hit
TCAGTGCACTACAGAACTTTGT4298932.7750647816145317No Hit
AAACCGTTACCATTACTGAGC2125531.3720817611045388No Hit
TTCAAGTAATCCAGGATAGGCT1814441.1712655340637486No Hit
TCCTGTACTGAGCTGCCCCGAG1625111.0490483741883658No Hit
TCCTGTACTGAGCTGCCCCGA1507530.9731475995718365No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1398410.9027079625063859No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC1251010.8075576463091036No Hit
TGGAGAGAAAGGCAGTTCCTGA1234620.7969774992095552No Hit
TAGCTTATCAGACTGATGTTGAC1230950.7946084241726215No Hit
AAGCTGCCAGTTGAAGAACTGT1196680.7724862984190201No Hit
TGAGGTAGTAGATTGTATAGTT1002700.6472674494641437No Hit
TCAGTGCACTACAGAACTTTGC977040.6307032899416045No Hit
TGAGGTAGTAGGTTGTATAGTT946840.6112084490382266No Hit
TGAGGTAGTAGTTTGTGCTGTT929090.5997503885734927No Hit
TAGCTTATCAGACTGATGTTGA926020.5977686282564938No Hit
TATTGCACTTGTCCCGGCCTGT853480.5509422786163931No Hit
AAAAGCTGGGTTGAGAGGGCGA759000.4899531207173482No Hit
TGGCTCAGTTCAGCAGGAAC733610.47356325281877976No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT677200.43714921389958916No Hit
TGGAGTGTGACAATGGTGTTT663440.42826679632242093No Hit
TGAGAACTGAATTCCATGGGTT655180.42293476367798705No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT645170.41647306310041043No Hit
TGAGGTAGTAGGTTGTATAGT631280.40750672733392296No Hit
CCCATAAAGTAGAAAGCACT627060.4047826138037159No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC623450.40245227023877567No Hit
AAACCGTTACCATTACTGAG613400.39596474867987No Hit
TTCACAGTGGCTAAGTTCCG594850.3839902685885567No Hit
TGAGGTAGTAGTTTGTACAGT594390.3836933273032735No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA578750.3735973236036433No Hit
TGAGGTAGTAGTTTGTGCTGT555300.3584597732995302No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC545950.35242411891388176No Hit
TGAGGTAGTAGATTGTATAGT525920.33949426251339626No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT519530.3353693607460921No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG519290.3352144348581182No Hit
TAGCTTATCAGACTGATGTTG486530.31406705114968564No Hit
CATTGCACTTGTCTCGGTCTGA481300.3106909578409218No Hit
TGAGGTAGTAGTTTGTACAGTT475420.3068952735855621No Hit
TTCAAGTAATCCAGGATAGGCC472260.3048554160605729No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA468300.3022991389090042No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT466550.3011694709758614No Hit
TCCTGTACTGAGCTGCCCCGAGA466520.30115010523986463No Hit
TGTAAACATCCCCGACTGGAAGCT433510.27984134039812597No Hit
TCCTGTACTGAGCTGCCCCGAGT382260.24675820807037352No Hit
TGTAAACATCCTTGACTGGAAGCT381890.2465193639930805No Hit
TCCTGTACTGAGCTGCCCCG379470.24495719462267734No Hit
AGCAGCATTGTACAGGGCTATGA367670.23734000513062897No Hit
TGAGGTAGTAGGTTGTGTGGTT360170.23249857113144567No Hit
TCGTACCGTGAGTAATAATGCG359340.23196278576886942No Hit
TGAGATGAAGCACTGTAGCT353030.2278895259642232No Hit
CGCGACCTCAGATCAGACGT342530.2211115183653666No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT334970.21623135289418988No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT306460.19782744845196115No Hit
TGAGGTAGTAGGTTGTATAG302910.1955358363590144No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT293630.1895453686906916No Hit
GGCTGGTCCGATGGTAGT284800.18384538706231984No Hit
AAGCTGCCAGTTGAAGAACTGC282750.18252206176920974No Hit
TGAGATGAAGCACTGTAGCTC275300.17771290399668768No Hit
TGAGGTAGTAGGTTGTGTGGT269920.17423998200794022No Hit
TGAGGGGCAGAGAGCGAGACTTT266730.17218075874695427No Hit
TACAGTATAGATGATGTACT260790.1683463430196011No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA260370.16807522271564684No Hit
TGTAAACATCCCCGACTGGAAG259390.16744260867308688No Hit
TACAGTACTGTGATAACTGAAG249790.1612455731541323No Hit
TGGAGAGAAAGGCAGTTCCTG248800.1606065038662401No Hit
TATTGCACTTGTCCCGGCCTGC231670.14954866861210547No Hit
GGCTGGTCCGATGGTAGTGGGT228750.1476637369750901No Hit
CGCGACCTCAGATCAGA226170.14599828367937107No Hit
AAAAGCTGGGTTGAGAGGGCGAT222750.14379058977574347No Hit
CAACGGAATCCCAAAAGCAGCT221190.14278357150391338No Hit
TGAGGTAGTAGATTGTATAGTC219520.14170554553342854No Hit
GGCTGGTCCGATGGTAGTG219010.1413763280214841No Hit
TCAGTGCATCACAGAACTTTGT218760.14121494688817798No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC217860.140633974808276No Hit
TTCAAGTAATTCAGGATAGGT214700.13859411728328677No Hit
TGAGGTAGTAGGTTGTATAGTC214370.1383810941873227No Hit
TAGCTTATCAGACTGATGTTGAT208820.13479843302792707No Hit
AGAGGTAGTAGGTTGCATAGTT208540.1346176861586242No Hit
TGAGGTAGTAGTTTGTGCTG207800.13413999800403814No Hit
TGAGGTAGTAGATTGTATAG203750.13152562364447917No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC201030.1297697969141087No Hit
TGAGGTAGTAGTTTGTGCTGTC200720.12956968430880914No Hit
CAACGGAATCCCAAAAGCAGCTG200460.1294018479301708No Hit
CTAGACTGAAGCTCCTTGAGG196990.12716187779988197No Hit
AAAAGCTGGGTTGAGAGGGCGAA182880.11805352663608515No Hit
CATTGCACTTGTCTCGGTCTG177820.11478717249796949No Hit
AAACCGTTACCATTACTG170950.11035241895471762No Hit
TCCTGTACTGAGCTGCCCCGAA168330.10866114467766959No Hit
TTCAAGTAATCCAGGATAGGCA167490.10811890406976106No Hit
AAGCTGCCAGTTGAAGAACT165800.10702796760861176No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC165740.10698923613661829No Hit
GGCTGGTCCGATGGTAGTGGGTT165740.10698923613661829No Hit
AAATGGATTTTTGGAAATAGGAGA163640.10563363461684697No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC159610.10303217074795248No Hit
AAAAGCTGGGTTGAGAGGGCG159410.1029030658413076No Hit
CGGACAGGATTGACAGA158920.10258675882002762No Hit
TGTAAACATCCCCGACTGGAAGC156150.10079865586299594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)