FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10976390
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8360827.617094509214778No Hit
TCAGTGCACTACAGAACTTTGT2918112.6585334522552495No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2654892.4187278331035977No Hit
TTCAAGTAATCCAGGATAGGCT1737681.5831070142369212No Hit
TAGCTTATCAGACTGATGTTGAC1711741.5594744720258664No Hit
TTCACAGTGGCTAAGTTCCG1586761.4456118997229508No Hit
TAGCTTATCAGACTGATGTTGA1195811.089438330817327No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1178811.0739505429380698No Hit
AAGCTGCCAGTTGAAGAACTGT954740.8698123882260014No Hit
TGGCTCAGTTCAGCAGGAAC882820.804289935033285No Hit
TAGCTTATCAGACTGATGTTG847100.7717473595599281No Hit
TCCTGTACTGAGCTGCCCCGAG831220.7572799435880103No Hit
AAACCGTTACCATTACTGAGC819480.7465842594878644No Hit
TGAGGGGCAGAGAGCGAGACTTT792030.7215760372945932No Hit
AAACCGTTACCATTACTGAG787110.717093689273067No Hit
TCGTACCGTGAGTAATAATGCG771360.7027447093261081No Hit
CGCGACCTCAGATCAGA705460.6427067551353405No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC662960.6039872854371975No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT624800.5692217568799942No Hit
TCCTGTACTGAGCTGCCCCGA581430.5297096768609716No Hit
TGGAGTGTGACAATGGTGTTT575070.5239154221014377No Hit
TGAGGTAGTAGTTTGTGCTGTT552670.5035079839546518No Hit
TATTGCACTTGTCCCGGCCTGT544540.4961011771629834No Hit
CGGACAGGATTGACAGA507960.4627751018322053No Hit
CCCATAAAGTAGAAAGCACT507200.4620827066093679No Hit
TGAGGTAGTAGGTTGTATAGT499400.4549765451118264No Hit
TGAGGTAGTAGGTTGTGTGGTT425650.3877868771062253No Hit
TGAGGTAGTAGGTTGTGTGGT423160.385518371705087No Hit
TGAGGTAGTAGGTTGTATAGTT422470.38488974972645834No Hit
TACAGTATAGATGATGTACT392520.3576039116685905No Hit
TGAGGTAGTAGATTGTATAGTT388000.3534859821853998No Hit
TGAGGGGCAGAGAGCGAGACT371430.33838994423485314No Hit
TGAGGTAGTAGATTGTATAGT369460.336595182933551No Hit
AGGAAACTCTGGTG334050.3043350318274041No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC320280.29178992364520573No Hit
TGAGGTAGTAGTTTGTACAGT308270.2808482570316835No Hit
TCAGTGCACTACAGAACTTTGC304370.27729517628291267No Hit
CGCGACCTCAGATCAGACGT303720.27670299615811755No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG301680.27484446161260667No Hit
CATTGCACTTGTCTCGGTCTGA299300.27267617130951066No Hit
TGAGATGAAGCACTGTAGCTC298480.271929113305923No Hit
AAAAGCTGGGTTGAGAGGGCGA271940.247749943287365No Hit
TGGCTCAGTTCAGCAGGAACAG263970.24048890391103087No Hit
TGTAAACATCCCCGACTGGAAG262320.23898567744039706No Hit
TACAGTACTGTGATAACTGAAG257140.23426645736895282No Hit
TGAGGTAGTAGGTTGTGTGG255090.23239881235998355No Hit
TGAGAACTGAATTCCATGGGTT250200.2279437957288325No Hit
AAACCGTTACCATTACTG244450.22270527924026026No Hit
TGTAAACATCCTTGACTGGAAGCT240490.21909753571073914No Hit
TCCTGTACTGAGCTGCCCCG227670.20741792155708752No Hit
TCCTGTACTGAGCTGCCCCGAGA220970.2013139110399685No Hit
CGCGACCTCAGATC217110.1977972721450313No Hit
TTCAAGTAATCCAGGATAGGCC213030.19408020305400955No Hit
TGTAAACATCCCCGACTGGAAGCT212340.19345158107538088No Hit
TTCACAGTGGCTAAGTTCCGA210220.19152016282220294No Hit
CTAGACTGAAGCTCCTTGAGG209440.19080954667244876No Hit
TTCAAGTAATTCAGGATAGGT207080.18865947729626956No Hit
TGAGATGAAGCACTGTAGCT198370.1807242636240148No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA183770.1674229869747704No Hit
TCAGTGCATCACAGAACTTTGT179210.1632686156377461No Hit
TCCTGTACTGAGCTGCCCCGAGT178310.16244867392649132No Hit
TGTAAACATCCCCGACTGGAAGC177590.16179272055748747No Hit
AACATTCAACGCTGTCGGTGA166520.15170743750905352No Hit
TGAGGTAGTAGGTTGTATAG166220.15143412360530192No Hit
CATTGCACTTGTCTCGGTCTG163560.1490107403253711No Hit
TACAGTACTGTGATAACTGA158500.14440084581542748No Hit
GGAAACTCTGGTGG148000.13483485918412155No Hit
TGAGGTAGTAGTTTGTGCTGT146420.13339540595769647No Hit
AAAAGCTGGGTTGAGAGGGCG146130.13313120251740326No Hit
TGGCTCAGTTCAGCAGGAACA143030.1303069588453034No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT142190.12954167991479895No Hit
CAACGGAATCCCAAAAGCAGCTG141840.1292228136937554No Hit
TGGAGAGAAAGGCAGTTCCTG141340.12876729052083608No Hit
TACAGTACTGTGATAACTGAA137530.1252962039431908No Hit
GCGCACGTGTTAGGAC135240.12320990781122027No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA134880.12288193112671834No Hit
TGAGGGGCAGAGAGCGAGACTT131350.11966593752590789No Hit
AAACCGTTACCATTACTGAGTT129060.11757964139393734No Hit
TAGTGGGTTATCAGAACT122150.11128431114419221No Hit
GTCTGAGCGTCGCT119190.10858761396050978No Hit
GGCTGGTCCGATGGTAGTGGGTTATC116630.1062553353151628No Hit
AGCTCGGTCTGAGGCCCCTCAGT116520.10615512021712055No Hit
TACAGTATAGATGATG115630.10534428896932416No Hit
AACCCGTAGATCCGATCTTGT115610.10532606804240739No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT114680.10447879494077743No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC113190.10312133588547784No Hit
TTCACAGTGGCTAAGTTCCA112820.10278424873751753No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT112470.10246538251647398No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC111640.1017092140494279No Hit
AAGCCGGCGGGAGC111020.10114436531500795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)