FastQCFastQC Report
Wed 8 May 2024
EGAF00002167575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11291016
Total Bases256.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT5675535.026589281248029No Hit
TGGAGTGTGACAATGGTGTTT4018843.559325396403654No Hit
TCAGTGCACTACAGAACTTTGT3862663.42100303462505No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3310942.932366759554676No Hit
CGCGACCTCAGATCAGA2235641.9800166787470677No Hit
TCCTGTACTGAGCTGCCCCGAG1794761.5895469459967109No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1329401.177396259114326No Hit
TCCTGTACTGAGCTGCCCCGA1148361.0170563924451086No Hit
TTCAAGTAATCCAGGATAGGCT1072380.9497639539258468No Hit
TGAGGGGCAGAGAGCGAGACTTT1029500.9117868577991564No Hit
TATTGCACTTGTCCCGGCCTGT970030.8591166640805398No Hit
TAGCTTATCAGACTGATGTTGAC951030.8422891261512694No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT863530.7647938856875236No Hit
TTCACAGTGGCTAAGTTCCG805970.7138153023607442No Hit
TGAGGTAGTAGGTTGTATAGTT799780.7083330676353661No Hit
TAGCTTATCAGACTGATGTTGA780120.6909209941780262No Hit
TGAGGTAGTAGGTTGTATAGT750460.6646523218105439No Hit
TGGCTCAGTTCAGCAGGAAC712700.6312098043258463No Hit
AAGCTGCCAGTTGAAGAACTGT693700.6143822663965759No Hit
TGAGGTAGTAGGTTGTGTGGTT668130.5917359429833418No Hit
TGAGGTAGTAGATTGTATAGTT603670.5346463064085641No Hit
TGAGGTAGTAGTTTGTGCTGTT589800.5223622037201967No Hit
TCCTGTACTGAGCTGCCCCGAGA552500.48932708978536565No Hit
TGAGGTAGTAGGTTGTGTGGT532120.4712773412064955No Hit
AAACCGTTACCATTACTGAGC529900.46931117624844393No Hit
TGAGGGGCAGAGAGCGAGACT525530.4654408425247117No Hit
CATTGCACTTGTCTCGGTCTGA524840.46482973719991183No Hit
TGAGGTAGTAGATTGTATAGT517330.4581784314183949No Hit
TCGTACCGTGAGTAATAATGCG516130.4571156395491779No Hit
CGCGACCTCAGATC509040.45083631092188686No Hit
TAGCTTATCAGACTGATGTTG477300.42272546598109506No Hit
CGCGACCTCAGATCAGACGT461650.4088648886867222No Hit
AAACCGTTACCATTACTGAG429810.38066547775682896No Hit
TCCTGTACTGAGCTGCCCCGAGT428720.37970010847562347No Hit
AAAAGCTGGGTTGAGAGGGCGA423510.3750858204434393No Hit
TCCTGTACTGAGCTGCCCCG389330.3448139653685727No Hit
TCAGTGCACTACAGAACTTTGC383710.33983655678107266No Hit
CCCATAAAGTAGAAAGCACT381140.33756041086116606No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG375610.33266271166385736No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA365900.3240629541221091No Hit
TGTAAACATCCCCGACTGGAAG363380.32183109119675324No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC363030.32152111023489827No Hit
TGAGGTAGTAGTTTGTACAGT350940.3108134821525361No Hit
TGGCTCAGTTCAGCAGGAACAG332110.2941365064047381No Hit
TGAGGTAGTAGGTTGTGTGG320720.28404884024608595No Hit
CGTGTATTTGACAAGCTGAGTTG304320.26952401803345244No Hit
AAATGGATTTTTGGAGCAGGGAGA282110.24985351185402624No Hit
TGGAGTGTGACAATGGTGTTTG272590.24142202969157073No Hit
CTAGACTGAAGCTCCTTGAGG268420.23772882794604136No Hit
TGAGGGGCAGAGAGCGAGACTT265270.23493899928934647No Hit
AAATGGATTTTTGGAGCAGGGA244080.21617186619875486No Hit
TGAGGTAGTAGGTTGTATAG243310.21548990808267385No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA240530.2130277735856543No Hit
TGAGATGAAGCACTGTAGCT230540.20418003127442205No Hit
AAATGGATTTTTGGAGCAGGGAG223940.19833467599372812No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT219440.19434920648416404No Hit
AAACCGTTACCATTACTG218880.19385323694519607No Hit
TGAGAACTGAATTCCATGGGTT214740.19018660499639714No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC212620.18830900602744693No Hit
TGTAAACATCCCCGACTGGAAGCT212340.18806102125796298No Hit
TGTAAACATCCCCGACTGGAAGC209010.18511177382088556No Hit
TGAGATGAAGCACTGTAGCTC208100.1843058233200626No Hit
AACCCGTAGATCCGATCTTGT201440.1784073284459078No Hit
CGCGACCTCAGATCAGAC200950.1779733550993108No Hit
AAATGGATTTTTGGAAATAGGAGA198140.1754846508055608No Hit
CATTGCACTTGTCTCGGTCTG192700.17066666099844335No Hit
TGAGGTAGTAGTTTGTACAGTT182570.16169492630246915No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA179790.15923279180544955No Hit
TGGAGTGTGACAATGGTGTTC178930.15847112429917734No Hit
TGTAAACATCCTTGACTGGAAGCT168490.1492248350369887No Hit
CGCGACCTCAGATCAGACG167830.1486402995089193No Hit
GTTTCCGTAGTGTAGTGGTTATC165370.1464615761770243No Hit
GGCTGGTCCGATGGTAGTGGGTTATC164010.14525707872524493No Hit
AAATGGATTTTTGGAGCAGGG159670.14141331479824312No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT159330.1411121904352983No Hit
AGCTCGGTCTGAGGCCCCTCAGT152260.13485057500582764No Hit
CAACGGAATCCCAAAAGCAGCTG150510.13330067019655273No Hit
TGGCTCAGTTCAGCAGGAACA149840.1327072780695732No Hit
TACAGTACTGTGATAACTGAAG149050.13200760675567194No Hit
TGAGGTAGTAGATTGTATAG147670.13078539610607232No Hit
TGAGGTAGTAGTTTGTGCTGT141080.12494889742428847No Hit
AAAAGCTGGGTTGAGAGGGCG139060.12315986444443972No Hit
TATTGCACTTGTCCCGGCCTG138220.12241591013598775No Hit
AACATTCAACGCTGTCGGTGA137920.12215021216868348No Hit
GGCTGGTCCGATGGTAGTG132740.11756249393322973No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC128970.11422355614410606No Hit
TTCAAGTAATTCAGGATAGGT128910.11417041655064522No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC126970.1124522363620776No Hit
TTCAAGTAATCCAGGATAGGCC123190.10910444197404379No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT122100.10813907269283828No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCA114780.10165604229061406No Hit
AAACCGTTACCATTACTGAGTT114670.1015586197026025No Hit
TGAAAAGAACTTTGAAGAGAG114610.10150548010914164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)