FastQCFastQC Report
Thu 9 May 2024
EGAF00002167576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11677131
Total Bases252.4 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT133834811.461274177706835No Hit
TCAGTGCACTACAGAACTTTGT3266662.7974851014345905No Hit
TGGAGTGTGACAATGGTGTTT2720112.3294334884142347No Hit
AAACCGTTACCATTACTGAGC2485572.128579357378109No Hit
TATTGCACTTGTCCCGGCCTGT1932421.6548756710873587No Hit
TCCTGTACTGAGCTGCCCCGAG1754311.50234676651311No Hit
TCCTGTACTGAGCTGCCCCGA1672141.4319784542966931No Hit
CGCGACCTCAGATCAGA1581281.3541682456075896No Hit
TTCAAGTAATCCAGGATAGGCT1339841.147405128879688No Hit
TGAGGTAGTAGGTTGTATAGT1185011.0148126282046506No Hit
TGAGGGGCAGAGAGCGAGACTTT1097860.9401795697932993No Hit
TGAGGTAGTAGGTTGTATAGTT1022160.8753520021313456No Hit
AAGCTGCCAGTTGAAGAACTGT886490.7591676414352122No Hit
TGAGGGGCAGAGAGCGAGACT848260.7264284352038184No Hit
AAACCGTTACCATTACTGAG844680.7233626136419982No Hit
TGAGGTAGTAGATTGTATAGT816180.6989559336107474No Hit
TGAGGTAGTAGATTGTATAGTT797480.6829417260112951No Hit
CGCGACCTCAGATC776310.6648122728091344No Hit
TCAGTGCACTACAGAACTTTGC636630.5451938494138672No Hit
TGAGGTAGTAGGTTGTGTGGTT623490.5339410853573536No Hit
CCCATAAAGTAGAAAGCACT613630.5254972304412787No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC611840.5239643196603686No Hit
TGAGGTAGTAGTTTGTGCTGTT608290.5209241893406865No Hit
TGAGGTAGTAGGTTGTGTGGT581410.4979048363848963No Hit
TCCTGTACTGAGCTGCCCCGAGT579780.49650894556205627No Hit
TAGCTTATCAGACTGATGTTGAC547650.46899362523208826No Hit
TAGCTTATCAGACTGATGTTGA529460.45341616874898466No Hit
TGAGGTAGTAGTTTGTACAGT525850.4503246559450262No Hit
TCCTGTACTGAGCTGCCCCG523700.44848345025845815No Hit
CATTGCACTTGTCTCGGTCTGA474270.40615284696215187No Hit
TCCTGTACTGAGCTGCCCCGAGA464850.39808579693077006No Hit
TGGAGTGTGACAATGGTGTTC460950.3947459354528094No Hit
TATTGCACTTGTCCCGGCCTGC457240.3915687851750571No Hit
TTCACAGTGGCTAAGTTCCG441760.3783121042317672No Hit
TGGCTCAGTTCAGCAGGAAC405680.3474141036869416No Hit
CGCGACCTCAGATCAGACGT391240.3350480524711078No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG373050.31947059598800426No Hit
TAGCTTATCAGACTGATGTTG349120.29897754850913294No Hit
TGAGGGGCAGAGAGCGAGACTT343200.2939078100605363No Hit
AAACCGTTACCATTACTG321330.27517889454181854No Hit
TGAGATGAAGCACTGTAGCT318000.27232716666448287No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT315450.2701434110827394No Hit
TTCAAGTAATCCAGGATAGGCC311330.2666151471624323No Hit
AAACCGTTACCATTACTGAGTT305950.26200785107232244No Hit
AAAAGCTGGGTTGAGAGGGCGA280060.23983630910709144No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT270250.23143527292791352No Hit
CGCGACCTCAGATCAGAC266920.22858354505057793No Hit
TGAGGTAGTAGGTTGTATAG257390.22042229379802283No Hit
TCGTACCGTGAGTAATAATGCG256380.21955735531270482No Hit
TGAGGTAGTAGGTTGTGTGG251550.21542106532846123No Hit
TGTAAACATCCCCGACTGGAAG251010.21495862296997437No Hit
TGTAAACATCCCCGACTGGAAGCT236330.20238704181703535No Hit
TGAGGTAGTAGTTTGTGCTGT233120.19963807890825236No Hit
AACCCGTAGATCCGATCTTGT225980.1935235632793706No Hit
TGAGGTAGTAGGTTGTATAGC220460.18879637472594937No Hit
TGAGGTAGTAGGTTGTATAGTC201420.17249099971559795No Hit
TGAGGTAGTAGTTTGTACAGTT201170.17227690603111329No Hit
CATTGCACTTGTCTCGGTCTG200600.17178877243048826No Hit
TGAGAACTGAATTCCATGGGTT200540.17173738994621196No Hit
TGTAAACATCCTTGACTGGAAGCT200180.17142909504055406No Hit
TTCAAGTAATTCAGGATAGGT197450.1690911920059816No Hit
TATTGCACTTGTCCCGGCCTG189660.16242003279743972No Hit
AAACCGTTACCATTACTGAGA183530.15717045565387594No Hit
GGCTGGTCCGATGGTAGT183100.15680221451656234No Hit
TCCTGTACTGAGCTGCCCCGAA179140.15341097055432537No Hit
TGAGGGGCAGAGAGCGAGACC178780.15310267564866747No Hit
AAGCTGCCAGTTGAAGAACTGC176250.15093604756168275No Hit
TGAGGTAGTAGGTTGTAT172910.14807575593696773No Hit
TACAGTATAGATGATGTACT172830.14800724595793263No Hit
AAGCTGCCAGTTGAAGAACT168830.14458174700617815No Hit
TACAGTACTGTGATAACTGAAG167170.14316016494120004No Hit
ACCAATATTACTGTGCTGCTT167160.14315160119382064No Hit
TGAGGGGCAGAGAGCGAGACTTC165890.1420640052766386No Hit
TGAGATGAAGCACTGTAGCTC156890.13435663263519096No Hit
TGAGGTAGTAGATTGTATAGTC155980.1335773316236668No Hit
TGAGGTAGTAGATTGTAT155590.13324334547587074No Hit
TGAGGTAGTAGATTGTATAG153050.13106815364150665No Hit
TGAGGTAGTAGATTGTATAGC150510.12889296180714252No Hit
AGCTCGGTCTGAGGCCCCTCAGT143610.122983976115366No Hit
CAACGGAATCCCAAAAGCAGCT139770.11969549712168169No Hit
AAACCGTTACCATTACTGAGG135690.1162014881908921No Hit
TGAGGTAGTAGGTTGTGTGGTC133290.1141461888198394No Hit
GGCTGGTCCGATGGTAGTGGGT133080.11396635012487229No Hit
CTAGACTGAAGCTCCTTGAGG130090.11140578965843578No Hit
TACCTGGTTGATCCTGCC125350.10734657340060672No Hit
TCCTGTACTGAGCTGCCCCA125170.10719242594777775No Hit
CCCATAAAGTAGAAAGCACC122920.10526558278741585No Hit
TGAGGTAGTAGGTTGTGTGGC122690.10506861659768997No Hit
TGGAGTGTGACAATGGTGTTTG121080.10368985326960878No Hit
GGCTGGTCCGATGGTAGTG120540.1032274109111219No Hit
TGAGGTAGTAGTTTGTGCTGTC119040.10194284880421399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)