FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14699325
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9307916.332202329018509No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5878593.999224454184121No Hit
TCAGTGCACTACAGAACTTTGT3641022.476998093449869No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3588602.4413365919863668No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT2585591.758985531648562No Hit
TTCAAGTAATCCAGGATAGGCT1428890.9720786498699772No Hit
AAACCGTTACCATTACTGAGC1380330.9390431193269079No Hit
TCCTGTACTGAGCTGCCCCGAG1128850.7679604335573232No Hit
TGAGGGGCAGAGAGCGAGACTTT1079670.7345031149389513No Hit
TATTGCACTTGTCCCGGCCTGT1030050.7007464628477839No Hit
TCCTGTACTGAGCTGCCCCGA999850.680201301760455No Hit
CGCGACCTCAGATCAGA988450.6724458436016619No Hit
AAATGGATTTTTGGAGCAGGGAGAT986060.6708199186017045No Hit
TGAGGTAGTAGGTTGTATAGTT980910.6673163563633024No Hit
TGAGGTAGTAGGTTGTATAGT919630.6256273672430537No Hit
AAGCTGCCAGTTGAAGAACTGT910830.6196406977871433No Hit
AAATGGATTTTTGGAGCAGGGAGA824130.5606583975794807No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT788640.536514431785133No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT778540.5296433679777812No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC712340.4846072863890009No Hit
TGAGGTAGTAGATTGTATAGTT705060.47965467802092954No Hit
TGAGGGGCAGAGAGCGAGACT670820.45636109141066006No Hit
AAATGGATTTTTGGAAATAGGAGA634730.4318089436079548No Hit
TGAGGTAGTAGATTGTATAGT614550.41808042206019663No Hit
TAGCTTATCAGACTGATGTTGAC586220.3988074282322488No Hit
TCAGTGCACTACAGAACTTTGC573580.3902083939228502No Hit
TGAGGTAGTAGTTTGTGCTGTT571310.38866410532456425No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC560150.3810719199691142No Hit
TGAGGTAGTAGGTTGTGTGGTT528760.35971719789854295No Hit
CGCGACCTCAGATCAGACGT522730.35561496871454984No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA496990.33810396055601194No Hit
AAACCGTTACCATTACTGAG486840.3311988815812971No Hit
CCCATAAAGTAGAAAGCACT483110.3286613500960078No Hit
TGGAGTGTGACAATGGTGTTT474790.32300122624678346No Hit
TCCTGTACTGAGCTGCCCCGAGT469660.3195112700753266No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC463430.31527298022188094No Hit
AAGACTTATCAGAACGGCTTGGAAAGGCC460350.3131776459123123No Hit
TTCACAGTGGCTAAGTTCCG454140.3089529621258119No Hit
TAGCTTATCAGACTGATGTTGA450230.30629297603801536No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT446520.3037690506196713No Hit
TGGCTCAGTTCAGCAGGAAC431110.2932855760383555No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT429640.29228553011787956No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT428350.2914079387999109No Hit
TGAGGTAGTAGGTTGTGTGGT425820.28968677133133663No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA397200.2702164895326826No Hit
TGAGGTAGTAGTTTGTACAGT386470.2629168346165555No Hit
AAAAGCTGGGTTGAGAGGGCGA385780.26244742530694437No Hit
AAATGGATTTTTGGAGCAGGGA369980.2516986324201962No Hit
AAATGGATTTTTGGAAATAGGAGAT355330.24173218838279986No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT351720.2392762932991821No Hit
AAATGGATTTTTGGAGCAGGGAG351070.23883409612346146No Hit
TGAGGGGCAGAGAGCGAGACTT345470.2350243973787912No Hit
CATTGCACTTGTCTCGGTCTGA341190.23211269905250753No Hit
TCCTGTACTGAGCTGCCCCGAGA331090.2252416352451558No Hit
GCCCAGATGGCGGAATTGGTAGACGCA290840.19785942551783842No Hit
TCCTGTACTGAGCTGCCCCG278760.18964136108290688No Hit
TTCAAGTAATCCAGGATAGGCC273890.18632828378173827No Hit
AAATGGATTTTTGGAGCAGGG272180.18516496505791932No Hit
AAACCGTTACCATTACTGAGTT269280.18319208535085796No Hit
TCGTACCGTGAGTAATAATGCG267910.18226006976510828No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC266010.18096749340530943No Hit
AAGACTTATCAGAACGGCTTGGAAAGGCT260920.17750474936774308No Hit
TAGCTTATCAGACTGATGTTG250290.17027312478634224No Hit
TGAGGTAGTAGTTTGTACAGTT247880.1686335937194395No Hit
CGCGACCTCAGATC246890.16796009340564957No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA246060.16739544162742168No Hit
TGAGAACTGAATTCCATGGGTT242210.16477627374046086No Hit
TGTAAACATCCCCGACTGGAAGCT211850.14412226411757004No Hit
TATTGCACTTGTCCCGGCCTGC211510.14389096097950077No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC210290.14306099089584046No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT208870.14209496014272763No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG204620.13920367091686184No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT200890.13666613943157255No Hit
TGTAAACATCCTTGACTGGAAGCT198320.13491775982910778No Hit
AAATGGATTTTTGGAAATAGGAG195570.13304692562413578No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT191860.13052300020579174No Hit
TGTAAACATCCCCGACTGGAAG182320.1240329062729071No Hit
TTCAAGTAATTCAGGATAGGT179980.12244099644031273No Hit
AGACTTATCAGAACGGCTTGGAAAGGCC173850.11827073692159333No Hit
TGAGATGAAGCACTGTAGCT173610.11810746411825032No Hit
AGCTCGGTCTGAGGCCCCTCAGT171670.116787675624561No Hit
GGCTGGTCCGATGGTAGTGGGTTATC168660.11473996254930073No Hit
TGAGGTAGTAGGTTGTGTGG167770.11413449257023707No Hit
TGAGGTAGTAGTTTGTGCTGT167730.11410728043634658No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA167690.11408006830245607No Hit
AAACCGTTACCATTACTG165260.11242693116860808No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCC158330.10771242897207865No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCC152080.10346053305168774No Hit
AAGCTGCCAGTTGAAGAACTGC150230.10220197185925205No Hit
GCACTCATAGCTCAGCTGGATAGAGTACTCGGCT149440.10166453221491464No Hit
TGAGGTAGTAGGTTGTATAGTC149400.10163732008102414No Hit
TGAGGTAGTAGGTTGTATAG149330.10158969884671576No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)