FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10986692
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT150816013.727152813603949No Hit
AAACCGTTACCATTACTGAGC2381622.167731652075074No Hit
TCAGTGCACTACAGAACTTTGT1859271.6922928211694659No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1854651.6880877337782838No Hit
TATTGCACTTGTCCCGGCCTGT1761331.6031486092447116No Hit
TTCAAGTAATCCAGGATAGGCT1667881.5180911597412579No Hit
TGAGGTAGTAGGTTGTATAGT1650781.5025268752414285No Hit
TCCTGTACTGAGCTGCCCCGAG1604251.460175637944524No Hit
TCCTGTACTGAGCTGCCCCGA1527111.3899634212008491No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1071960.9756894978033424No Hit
TGAGGTAGTAGATTGTATAGT1037500.9443242788639202No Hit
TGAGGTAGTAGGTTGTGTGGT1008620.9180379317086527No Hit
TGAGGTAGTAGGTTGTATAGTT1006970.9165361147832304No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT911130.8293033062181047No Hit
CGCGACCTCAGATCAGA910000.8282747891722094No Hit
AAGCTGCCAGTTGAAGAACTGT895480.8150588002284946No Hit
TGAGGTAGTAGGTTGTGTGGTT871010.7927864001284464No Hit
TGAGGTAGTAGATTGTATAGTT739920.6734693208838475No Hit
TGAGGGGCAGAGAGCGAGACTTT727450.6621192256959602No Hit
AAACCGTTACCATTACTGAG719940.6552836832050994No Hit
TGAGGTAGTAGTTTGTGCTGTT693160.6309087394094601No Hit
TGAGGTAGTAGTTTGTACAGT672340.6119585403868607No Hit
TGAGGGGCAGAGAGCGAGACT634960.5779355605854792No Hit
AAACCGTTACCATTACTGAGG611360.5564550275915626No Hit
CCCATAAAGTAGAAAGCACT583930.5314884589465145No Hit
TGGAGTGTGACAATGGTGTTT576570.5247894452670558No Hit
TAGCTTATCAGACTGATGTTGAC570940.5196650638791003No Hit
TCCTGTACTGAGCTGCCCCG534970.48692545490489764No Hit
TCCTGTACTGAGCTGCCCCGAGA494950.4504995680228407No Hit
CATTGCACTTGTCTCGGTCTGA464680.42294805388191464No Hit
TCCTGTACTGAGCTGCCCCGAGT419530.3818528816499088No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT410060.3732333626900618No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT408640.3719408899421227No Hit
TTCAAGTAATTCAGGATAGGT396750.36111870615832314No Hit
TAGCTTATCAGACTGATGTTGA393690.35833351840572214No Hit
TCGTACCGTGAGTAATAATGCG393480.3581423780697593No Hit
TGAGGTAGTAGGTTGTATAG371690.33830929273342697No Hit
TATTGCACTTGTCCCGGCCTGC363760.33109146957064056No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG344580.3136339855527032No Hit
TTCAAGTAATCCAGGATAGGCC332690.3028118017689037No Hit
AAACCGTTACCATTACTG330560.3008730926469951No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC325730.2964768649198503No Hit
TGAGGTAGTAGGTTGTGTGG314490.28624630598545947No Hit
TCAGTGCACTACAGAACTTTGC311350.28338830286677735No Hit
CGCGACCTCAGATCAGACGT310390.2825145184738045No Hit
CGCGACCTCAGATC295420.2688889430958836No Hit
TTCACAGTGGCTAAGTTCCG289850.2638191732324889No Hit
TAGCTTATCAGACTGATGTTG278520.2535066970112569No Hit
AAACCGTTACCATTACTGAGTT271730.24732649281512578No Hit
TGAGGTAGTAGGTTGTATAGC264540.24078221178858933No Hit
TGGCTCAGTTCAGCAGGAAC247230.22502678695279707No Hit
TGAGGTAGTAGTTTGTACAGTT242490.22071247651249348No Hit
TATTGCACTTGTCCCGGCCTG238640.2172082370198418No Hit
TGTAAACATCCCCGACTGGAAGCT237200.2158975604303825No Hit
AAAAGCTGGGTTGAGAGGGCGA233410.21244793246229166No Hit
CATTGCACTTGTCTCGGTCTG228830.2082792527541502No Hit
TGAGGTAGTAGATTGTATAG197640.17989036190329172No Hit
AAGCTGCCAGTTGAAGAACT190510.17340069240131606No Hit
TGTAAACATCCCCGACTGGAAG189850.1727999656311472No Hit
TGAGGTAGTAGGTTGTAT189420.17240858303846143No Hit
TGAGGTAGTAGGTTGTGTGGC189040.17206271004957635No Hit
TGAGGGGCAGAGAGCGAGACTT187410.17057909696567447No Hit
TACAGTATAGATGATGTACT183860.16734791509582686No Hit
TGTAAACATCCTTGACTGGAAGCT183150.16670167872185732No Hit
TACAGTACTGTGATAACTGAAG182390.16600993274408712No Hit
CAACGGAATCCCAAAAGCAGCT180680.16445350429410416No Hit
AAACCGTTACCATTACTGAGA174600.1589195364719426No Hit
TGAGGTAGTAGTTTGTGCTGT172660.15715376384447657No Hit
TGAGATGAAGCACTGTAGCT172370.1568898081424327No Hit
TAGCAGCACGTAAATATTGGCG162820.14819747381650455No Hit
TGAGGTAGTAGATTGTATAGC161340.1468503895440047No Hit
TGAGGTAGTAGATTGTAT160840.14639529350599798No Hit
TGAGGTAGTAGGTTGTATAGTC159730.14538498030162308No Hit
AAGCTGCCAGTTGAAGAACTGC156970.14287285017182608No Hit
TGAGGTAGTAGGTTGTGTGGTC155700.14171690623528901No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC154800.14089773336687694No Hit
GCATTGGTGGTTCAGTGGTAGAATT153770.13996023552858314No Hit
AGAGGTAGTAGGTTGCATAGT152800.1390773492148501No Hit
ACCAATATTACTGTGCTGCTT151940.13829458402947858No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC151780.13814895329731644No Hit
TGGCTCAGTTCAGCAGGAACAG138070.12567021993517247No Hit
TAGCAGCACGTAAATATTGGC136090.12386803962466592No Hit
AGCTCGGTCTGAGGCCCCTCAGT133330.12135590949486888No Hit
TCCTGTACTGAGCTGCCCCGAA131280.11949001573904136No Hit
CAACGGAATCCCAAAAGCAGCTG128590.11704159905456528No Hit
TGAGAACTGAATTCCATGGGTT128070.11656829917503832No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT126150.11482073038909255No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT125920.11461138621160948No Hit
AGAAGCCGGCGGGAGC123770.11265447324818062No Hit
CTAGACTGAAGCTCCTTGAGG122010.111052535194397No Hit
TATTGCACTTGTCCCGGCCTGTA121370.11047001226574842No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT120590.10976006244645795No Hit
TGAGGTAGTAGATTGTATAGTC116420.10596456148948201No Hit
GGCTGGTCCGATGGTAGT114320.10405315812985383No Hit
TGAGGTAGTAGTTTGTGCTGTC112990.10284260266875597No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA111380.10137719342637437No Hit
AAGCCGTTACCATTACTGAGT111110.10113144156585076No Hit
TTCAAGTAATCCAGGATAGGCG110700.10075826281468526No Hit
AGACCGTTACCATTACTGAGT110580.10064903976556366No Hit
AGAGGTAGTAGGTTGCATAGTT110030.10014843412375626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)