FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9338972
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8320358.909278237476245No Hit
TCAGTGCACTACAGAACTTTGT2004962.1468744097316064No Hit
TGAGGTAGTAGGTTGTATAGT1443481.545651919718787No Hit
AAACCGTTACCATTACTGAGC1370341.4673349486431697No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1330641.4248249164897377No Hit
TTCAAGTAATCCAGGATAGGCT1256851.3458119373310038No Hit
TATTGCACTTGTCCCGGCCTGT1173411.2564659150921536No Hit
TCCTGTACTGAGCTGCCCCGAG954401.0219540223484984No Hit
TGAGGTAGTAGATTGTATAGT906080.9702138522312733No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC895100.9584566695349338No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT889500.9524602922034674No Hit
TGAGGTAGTAGGTTGTATAGTT873580.9354134480754412No Hit
CGCGACCTCAGATCAGA758240.811909490680559No Hit
TCCTGTACTGAGCTGCCCCGA752360.8056132944825191No Hit
TGAGGTAGTAGGTTGTGTGGT707810.7579099712473707No Hit
TGAGGTAGTAGATTGTATAGTT626170.6704913560079203No Hit
TGAGGTAGTAGGTTGTGTGGTT616190.659804954977914No Hit
TGAGGTAGTAGTTTGTGCTGTT568420.6086537147771724No Hit
CATTGCACTTGTCTCGGTCTGA546030.58467891326797No Hit
TGAGGTAGTAGTTTGTACAGT544210.5827300906352434No Hit
AAGCTGCCAGTTGAAGAACTGT517400.5540224341608476No Hit
CCCATAAAGTAGAAAGCACT476080.5097777357079559No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT442110.47340328250261376No Hit
TGAGGGGCAGAGAGCGAGACTTT434590.46535100437178734No Hit
AAACCGTTACCATTACTGAG396400.4244578525345188No Hit
CGCGACCTCAGATC395790.42380467571805547No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT387770.4152170067540624No Hit
TAGCTTATCAGACTGATGTTGAC383780.41094458790539257No Hit
CGCGACCTCAGATCAGACGT374940.40147887797500625No Hit
TCAGTGCACTACAGAACTTTGC354570.37966705543179696No Hit
TCCTGTACTGAGCTGCCCCGAGA338500.3624595940538209No Hit
TGAGGGGCAGAGAGCGAGACT334840.3585405331550411No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC314710.3369856982117518No Hit
TCCTGTACTGAGCTGCCCCGAGT293110.31385681421895256No Hit
TAGCTTATCAGACTGATGTTGA292560.31326788430246927No Hit
TGGAGTGTGACAATGGTGTTT287480.3078283134374961No Hit
TGAGGTAGTAGGTTGTATAG275330.2948183161915465No Hit
TTCAAGTAATTCAGGATAGGT274780.29422938627506323No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC270340.2894751156765434No Hit
TTCAAGTAATCCAGGATAGGCC265930.2847529685280136No Hit
TATTGCACTTGTCCCGGCCTGC254870.2729101232983673No Hit
TGAGGTAGTAGTTTGTACAGTT244650.26196673466844106No Hit
TCCTGTACTGAGCTGCCCCG244530.261838240868481No Hit
AAACCGTTACCATTACTGAGTT243250.2604676403355744No Hit
TGAGGTAGTAGGTTGTATAGC239530.2564843325368145No Hit
TGAGATGAAGCACTGTAGCT237460.25426781448750463No Hit
TCGTACCGTGAGTAATAATGCG237250.2540429503375746No Hit
TTCACAGTGGCTAAGTTCCG232670.24913877030576814No Hit
TGGCTCAGTTCAGCAGGAAC200140.214306242699946No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC191830.2054080470527163No Hit
CATTGCACTTGTCTCGGTCTG177390.18994595979086348No Hit
GCATTGGTGGTTCAGTGGTAGAATT175930.18838261855801688No Hit
TGTAAACATCCCCGACTGGAAGCT171350.18347843852621037No Hit
AAACCGTTACCATTACTG164030.17564031672865063No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT161140.17254575771294742No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC158780.17001871298040083No Hit
TGAGGTAGTAGGTTGTGTGG155590.16660291946479763No Hit
TGAGGTAGTAGGTTGTAT154410.16533939709852433No Hit
TGAGGTAGTAGGTTGTATAGTC150000.1606172499499945No Hit
CAACGGAATCCCAAAAGCAGCT148840.15937514321704788No Hit
TGAGGTAGTAGATTGTATAGC148310.15880762893389122No Hit
TGAGGTAGTAGATTGTATAG147890.15835790063403124No Hit
TGTAAACATCCTTGACTGGAAGCT143700.15387132545209473No Hit
AGAGGTAGTAGGTTGCATAGT142600.1526934656191281No Hit
TAGCTTATCAGACTGATGTTG142110.15216878260262479No Hit
AAAAGCTGGGTTGAGAGGGCGA140040.14995226455331484No Hit
GGTGGGCGTAGCTC136190.14582975513793167No Hit
TGAGGTAGTAGTTTGTGCTGT134170.1436667761719384No Hit
TGAGGTAGTAGGTTGTGTGGC133570.14302430717213843No Hit
TGAGGTAGTAGATTGTAT132070.14141813467263847No Hit
TGAGGGGCAGAGAGCGAGACTT129980.1391802009900019No Hit
TGTAAACATCCCCGACTGGAAG127460.136481831190842No Hit
TACAGTATAGATGATGTACT123250.1319738403755788No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT120780.12932900965973557No Hit
TGAGGTAGTAGGTTGTGTGGTC118000.126352236627329No Hit
AGCTCGGTCTGAGGCCCCTCAGT117970.126320113177339No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT117210.1255063191109257No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG115010.12315059944499245No Hit
TGAGATGAAGCACTGTAGCTC114170.12225114284527248No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT113840.1218977848953825No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT113070.12107328301230585No Hit
TATTGCACTTGTCCCGGCCTG111110.1189745509462926No Hit
GGCTGGTCCGATGGTAGT108410.11608344044719268No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA107410.1150126587808594No Hit
TAGCAGCACGTAAATATTGGCG107080.11465930083096941No Hit
CAACGGAATCCCAAAAGCAGCTG104330.11171465124855284No Hit
TGAGGTAGTAGATTGTATAGTC103950.1113077542153462No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG102730.11000140058241956No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC101970.10918760651600626No Hit
AGAGGTAGTAGGTTGCATAGTT101040.10819177956631629No Hit
GGCTGGTCCGATGGTAGTGGGTT99700.10675693213342968No Hit
GGCTGGTCCGATGGTAGTGGGT99470.10651065235017301No Hit
TGGCTCAGTTCAGCAGGAACAG99300.10632861946689635No Hit
TGAGGTAGTAGTTTGTGCTGTC98560.10553624103380971No Hit
TATTGCACTTGTCCCGGCCTGTA98530.10550411758381971No Hit
AAACCGTTACCATTACTGAGA96660.10350175586777645No Hit
TACAGTACTGTGATAACTGAAG96620.10345892460112312No Hit
AAGCTGCCAGTTGAAGAACTGC96500.10333043080116312No Hit
TCCTGTACTGAGCTGCCCCGAA96160.1029663650346098No Hit
TAGCAGCACGTAAATATTGGC94950.10167071921834653No Hit
AAGCTGCCAGTTGAAGAACT93920.10056781410202323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)