FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10841119
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT140840712.991343421283355No Hit
TCAGTGCACTACAGAACTTTGT2695732.4865791068246734No Hit
AAACCGTTACCATTACTGAGC2235352.0619181470104704No Hit
TGAGGTAGTAGGTTGTATAGT2079841.9184735450279624No Hit
TTCAAGTAATCCAGGATAGGCT1895431.7483711782888833No Hit
TCCTGTACTGAGCTGCCCCGAG1691611.5603647557046463No Hit
TGAGGTAGTAGATTGTATAGT1383631.2762796903161011No Hit
TATTGCACTTGTCCCGGCCTGT1338451.2346050255513292No Hit
TGAGGTAGTAGGTTGTATAGTT1288561.1885857908210398No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1179371.0878674055694804No Hit
TCCTGTACTGAGCTGCCCCGA1159021.0690962805592301No Hit
TGAGGTAGTAGGTTGTGTGGT1107861.0219055800420602No Hit
TGAGGTAGTAGATTGTATAGTT966170.8912087396144254No Hit
TGAGGTAGTAGGTTGTGTGGTT964600.8897605496259197No Hit
TGAGGTAGTAGTTTGTGCTGTT914630.8436675217751969No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT908700.8381976067230698No Hit
AAACCGTTACCATTACTGAGG874050.8062359614353464No Hit
TGAGGTAGTAGTTTGTACAGT766770.7072793869341347No Hit
AAACCGTTACCATTACTGAG717330.6616752385062833No Hit
AAGCTGCCAGTTGAAGAACTGT714160.6587511861091092No Hit
TAGCTTATCAGACTGATGTTGAC689270.6357923015142625No Hit
TGAGGGGCAGAGAGCGAGACTTT629320.580493581889471No Hit
CGCGACCTCAGATCAGA619640.5715646143170277No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC609200.5619346121004667No Hit
CATTGCACTTGTCTCGGTCTGA608870.561630215478679No Hit
CCCATAAAGTAGAAAGCACT608060.5608830601342906No Hit
TGGAGTGTGACAATGGTGTTT562870.5191991712294645No Hit
TGAGGGGCAGAGAGCGAGACT532860.49151752692687906No Hit
TCGTACCGTGAGTAATAATGCG518180.47797648932734715No Hit
TCCTGTACTGAGCTGCCCCG488080.45021182776427415No Hit
TCCTGTACTGAGCTGCCCCGAGA479340.4421499293569234No Hit
TCAGTGCACTACAGAACTTTGC449510.41463431957531316No Hit
TGAGGTAGTAGGTTGTATAG446660.41200543965987274No Hit
TCCTGTACTGAGCTGCCCCGAGT428310.39507914266045785No Hit
TAGCTTATCAGACTGATGTTGA417680.3852738817828676No Hit
TTCAAGTAATCCAGGATAGGCC384370.35454827126240385No Hit
TTCAAGTAATTCAGGATAGGT377270.3479991318239381No Hit
TTCACAGTGGCTAAGTTCCG361350.3333143008576882No Hit
TGAGGTAGTAGGTTGTATAGC327930.3024872247966285No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT325180.29995058628172977No Hit
AAACCGTTACCATTACTG321670.29671291312271364No Hit
CGCGACCTCAGATCAGACGT320000.29517248173366606No Hit
TGAGGTAGTAGTTTGTACAGTT319500.2947112747309572No Hit
TGAGGTAGTAGGTTGTGTGG315760.291261446350695No Hit
AAACCGTTACCATTACTGAGTT312190.2879684283513538No Hit
TATTGCACTTGTCCCGGCCTGC279620.25792540419489907No Hit
TGAGATGAAGCACTGTAGCT267950.24716083275167444No Hit
CATTGCACTTGTCTCGGTCTG263570.2431206594079449No Hit
TGTAAACATCCCCGACTGGAAGCT262860.2424657454640983No Hit
TGGCTCAGTTCAGCAGGAAC261900.2415802280188973No Hit
TAGCTTATCAGACTGATGTTG255140.23534470934227358No Hit
TGAGGTAGTAGATTGTATAG253310.23365669171235923No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG224980.2075247029388756No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT224900.2074509098184422No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC219550.20251599488945743No Hit
TGAGGTAGTAGATTGTATAGC217090.20024685643612988No Hit
AAAAGCTGGGTTGAGAGGGCGA215240.1985403905261071No Hit
TGAGGTAGTAGGTTGTAT213320.19676935563570516No Hit
TTCAAGTAATCCAGGATAGGCG206980.1909212508413569No Hit
TGAGGTAGTAGGTTGTGTGGC206130.19013719893675182No Hit
TGAGGTAGTAGGTTGTATAGTC203420.18763745698206982No Hit
CGCGACCTCAGATC199840.18433521484267446No Hit
TGAGGTAGTAGTTTGTGCTGT195360.18020280009840312No Hit
CAACGGAATCCCAAAAGCAGCT192270.1773525408216624No Hit
TGAGGTAGTAGATTGTAT190950.17613495433451104No Hit
CTAGACTGAAGCTCCTTGAGG189150.17447460912475918No Hit
TACAGTACTGTGATAACTGAAG188830.17417943664302551No Hit
TGTAAACATCCCCGACTGGAAG188770.17412409180270044No Hit
TACAGTATAGATGATGTACT187490.17294340187576576No Hit
TGTAAACATCCTTGACTGGAAGCT186340.17188262576953542No Hit
AGAGGTAGTAGGTTGCATAGT186000.1715690050076934No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC185720.17131072908617642No Hit
TCAGTGCACTACAGAACTTTGG177600.16382072736218467No Hit
TGAGGTAGTAGGTTGTGTGGTC171660.1583415881700035No Hit
TGAGGGGCAGAGAGCGAGACTT169190.15606322557662175No Hit
TAGCAGCACGTAAATATTGGCG168950.1558418462153215No Hit
TGGCTCAGTTCAGCAGGAACAG168940.15583262207526732No Hit
AAACCGTTACCATTACTGAGA168270.15521460469163745No Hit
TATTGCACTTGTCCCGGCCTG165320.15249348337565521No Hit
TGAGGTAGTAGATTGTATAGTC149090.13752270406772585No Hit
TGAGGTAGTAGTTTGTGCTGTC147670.1362128761800327No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC146580.1352074449141274No Hit
CAACGGAATCCCAAAAGCAGCTG143460.13232951321722417No Hit
AGCTCGGTCTGAGGCCCCTCAGT142430.13137942679164394No Hit
TGAGATGAAGCACTGTAGCTC139120.1283262364337113No Hit
AAGCTGCCAGTTGAAGAACT139000.1282155467530612No Hit
TAGCAGCACGTAAATATTGGC134730.1242768389499276No Hit
AGAGGTAGTAGGTTGCATAGTT133410.12305925246277621No Hit
TCCTGTACTGAGCTGCCCCGAA131870.12163873489443294No Hit
TGAGAACTGAATTCCATGGGTT129600.11954485510213475No Hit
TTCAAGTAATCCAGGATAGGCA128280.11832726861498337No Hit
AAGCTGCCAGTTGAAGAACTGC127180.11731261320902389No Hit
ACCAATATTACTGTGCTGCTT125520.1157814059600305No Hit
TGAGGTAGTAGTTTGTACAGC122820.1132908881454027No Hit
TATTGCACTTGTCCCGGCCTGTA113550.10474011031518056No Hit
TGGAGAGAAAGGCAGTTCCTG111920.1032365754863497No Hit
TGAGGTAGTAGGTTGTATAGG111720.10305209268526615No Hit
CGTGTATTTGACAAGCTGAGTTG109310.10082907493220948No Hit
TGTAAACATCCCCGACTGGAAGC108770.10033097136928391No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)