FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11480193
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT130072611.330175372487204No Hit
TCAGTGCACTACAGAACTTTGT3715523.236461268551844No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2264561.9725800777042686No Hit
TCCTGTACTGAGCTGCCCCGAG2131201.8564147832706297No Hit
AAACCGTTACCATTACTGAGC1977761.7227584936943134No Hit
TATTGCACTTGTCCCGGCCTGT1796011.564442339950208No Hit
TTCAAGTAATCCAGGATAGGCT1453271.2658933521413795No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1418371.2354931663605306No Hit
TCCTGTACTGAGCTGCCCCGA1300491.1328119657918643No Hit
TGAGGTAGTAGGTTGTGTGGT1142380.9950878003531822No Hit
TGAGGTAGTAGTTTGTGCTGTT1056240.9200542186006803No Hit
AAGCTGCCAGTTGAAGAACTGT1004080.8746194423734862No Hit
TGAGGTAGTAGGTTGTGTGGTT963670.8394196857143429No Hit
TGGAGTGTGACAATGGTGTTT889720.7750043923477593No Hit
TGAGGGGCAGAGAGCGAGACTTT865980.7543252974928208No Hit
TGAGGTAGTAGGTTGTATAGT808070.7038818946684956No Hit
TAGCTTATCAGACTGATGTTGAC775960.6759119816191244No Hit
TGAGGTAGTAGTTTGTACAGT711540.6197979424213512No Hit
CATTGCACTTGTCTCGGTCTGA688560.5997808573427293No Hit
TGAGGGGCAGAGAGCGAGACT674700.5877078895799052No Hit
TCCTGTACTGAGCTGCCCCGAGA669330.5830302678709322No Hit
TGAGGTAGTAGGTTGTATAGTT667200.5811748983662557No Hit
TCCTGTACTGAGCTGCCCCGAGT659580.5745373792931879No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC633130.5514976969463842No Hit
TCAGTGCACTACAGAACTTTGC598500.5213326988492266No Hit
TAGCTTATCAGACTGATGTTGA589590.5135715052874111No Hit
TGAGGTAGTAGATTGTATAGT583630.5083799549362977No Hit
AAACCGTTACCATTACTGAG562370.48986110250933934No Hit
TGAGGTAGTAGATTGTATAGTT559250.4871433781644612No Hit
CCCATAAAGTAGAAAGCACT500580.4360379655638193No Hit
TCCTGTACTGAGCTGCCCCG484050.4216392529289359No Hit
CGCGACCTCAGATCAGA480870.4188692646543486No Hit
TCGTACCGTGAGTAATAATGCG464970.40501932328141177No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT443480.38630012579056816No Hit
CGCGACCTCAGATCAGACGT438840.382258381893057No Hit
TGGCTCAGTTCAGCAGGAAC364620.317607900842782No Hit
TAGCTTATCAGACTGATGTTG361880.3152211813860621No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG354220.3085488196931881No Hit
TATTGCACTTGTCCCGGCCTGC351900.30652794774443254No Hit
TGAGGTAGTAGTTTGTACAGTT332600.2897163836879746No Hit
AAACCGTTACCATTACTGAGTT332200.2893679574899133No Hit
CGCGACCTCAGATC328330.28599693402367016No Hit
TGAGATGAAGCACTGTAGCT325750.28374958504617476No Hit
TGAGGTAGTAGGTTGTGTGG316220.27544833087736414No Hit
AAAAGCTGGGTTGAGAGGGCGA315440.2747688997911446No Hit
TGTAAACATCCCCGACTGGAAGCT310940.27084910506295495No Hit
TGAGGTAGTAGTTTGTGCTGT291290.2537326680831934No Hit
TTCAAGTAATTCAGGATAGGT285510.24869790952120754No Hit
TTCAAGTAATCCAGGATAGGCC283690.2471125703200286No Hit
AAACCGTTACCATTACTG277640.24184262407435136No Hit
TGAGGTAGTAGGTTGTATAG276030.24044020862715462No Hit
CATTGCACTTGTCTCGGTCTG265280.23107625455425704No Hit
AAATGGATTTTTGGAGCAGGGAGA263600.2296128645223996No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC262520.22867211378763408No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT244660.2131148840441968No Hit
TGAGAACTGAATTCCATGGGTT243250.21188668169603073No Hit
TGAGGGGCAGAGAGCGAGACTT241760.21058879410825235No Hit
TTCACAGTGGCTAAGTTCCG238940.2081323894119202No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC238020.20733100915637917No Hit
TGTAAACATCCTTGACTGGAAGCT232630.2026359661375031No Hit
CTAGACTGAAGCTCCTTGAGG223960.1950838282945243No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC220400.19198283513177872No Hit
TGTAAACATCCCCGACTGGAAG212720.18529305212900166No Hit
TGGCTCAGTTCAGCAGGAACAG201870.17584199150658877No Hit
TCCTGTACTGAGCTGCCCCGAA201360.1753977481040606No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC199240.1735510892543357No Hit
TGAGGTAGTAGGTTGTGTGGC198640.17302844995724376No Hit
AGCTCGGTCTGAGGCCCCTCAGT198110.17256678524481253No Hit
AAGCTGCCAGTTGAAGAACT184890.16105129939888643No Hit
AAACCGTTACCATTACTGAGG184170.16042413224237606No Hit
TATTGCACTTGTCCCGGCCTG179210.15610364738641588No Hit
TGAGGTAGTAGTTTGTGCT173710.15131278716307295No Hit
TGAGGTAGTAGTTTGTGCTGTC172450.15021524463917985No Hit
TGAGATGAAGCACTGTAGCTC171600.14947483896829958No Hit
TGAGGTAGTAGGTTGTGTGGTC170820.14879540788208No Hit
AAGCTGCCAGTTGAAGAACTGC166400.14494529839350262No Hit
TGGAGTGTGACAATGGTGTTTG164090.14293313709969857No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT163120.1420882035693999No Hit
GCATTGGTGGTTCAGTGGTAGAATT160010.1393791898794733No Hit
AAACCGTTACCATTACTGAGA156780.13656564833012824No Hit
GCGCGGGACATGTGGCGTACGGAAG155200.1351893648477861No Hit
CGTGTATTTGACAAGCTGAGTTG152400.13275038146135695No Hit
TACAGTACTGTGATAACTGAAG143120.1246668936663347No Hit
TAGCAGCACGTAAATATTGGCG141010.12282894547156133No Hit
TGAGGTAGTAGATTGTATAG140770.12261988975272455No Hit
TACAGTATAGATGATGTACT140710.12256762582301534No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA137360.11964955641425193No Hit
AAATGGATTTTTGGAAATAGGAGA135390.1179335573888No Hit
TGTAAACATCCCCGACTGGAAGC128210.11167930713359958No Hit
TGGAGTGTGACAATGGTGTTC127170.1107733990186402No Hit
AGAAGCCGGCGGGAGC126720.11038141954582122No Hit
TGAGGTAGTAGGTTGTATAGC126700.11036399823591816No Hit
CAACGGAATCCCAAAAGCAGCTG124210.10819504515298653No Hit
AACCCGTAGATCCGATCTTGT121230.10559926997742981No Hit
AAAAGCTGGGTTGAGAGGGCGT121130.1055121634279145No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT119860.10440591024906987No Hit
TGAGGGGCAGAGAGCGAGACC118770.1034564488593528No Hit
CAACGGAATCCCAAAAGCAGCT118190.10295123087216392No Hit
AAAAGCTGGGTTGAGAGGGCG118000.10278572842808478No Hit
TAGCTTATCAGACTGATGTTGACT117940.10273346449837559No Hit
TCAGTGCATCACAGAACTTTGT116200.10121781053680891No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)