FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10347163
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9748669.42157768269428No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2419362.338186805407434No Hit
TCCTGTACTGAGCTGCCCCGAG1986321.9196759536889485No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1966661.9006755764841048No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1849871.7878040579818837No Hit
AAACCGTTACCATTACTGAGC1783191.7233612730368701No Hit
TATTGCACTTGTCCCGGCCTGT1633331.578529303152951No Hit
TTCAAGTAATCCAGGATAGGCT1474241.4247770137572975No Hit
TCCTGTACTGAGCTGCCCCGA1340571.2955918448370825No Hit
TCAGTGCACTACAGAACTTTGT1200971.1606756364039108No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT995580.9621767821769116No Hit
AAACCGTTACCATTACTGAGG833030.805080581024963No Hit
TAGCTTATCAGACTGATGTTGAC703610.6800028181637807No Hit
TGAGGTAGTAGGTTGTATAGTT657960.6358844448473461No Hit
AAGCTGCCAGTTGAAGAACTGT655540.6335456395149086No Hit
TGAGGTAGTAGATTGTATAGTT640830.6193291823082328No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC640000.6185270300661158No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC618820.5980576511648652No Hit
CATTGCACTTGTCTCGGTCTGA573070.5538426329999827No Hit
AAACCGTTACCATTACTGAG545710.5274006024646563No Hit
TCCTGTACTGAGCTGCCCCG545430.5271299968890023No Hit
TGAGGTAGTAGTTTGTGCTGTT525570.5079363299872632No Hit
TGAGGGGCAGAGAGCGAGACTTT478910.4628418437015054No Hit
TGGAGTGTGACAATGGTGTTT477670.4616434475807524No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC469310.4535639382505137No Hit
TCCTGTACTGAGCTGCCCCGAGA469060.45332232612939405No Hit
CGCGACCTCAGATCAGA433200.418665483476002No Hit
TGAGGTAGTAGGTTGTATAGT432280.41777635087028203No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT410680.3969010636055506No Hit
CGCGACCTCAGATCAGACGT409460.3957219964544871No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG405890.39227177536489954No Hit
TATTGCACTTGTCCCGGCCTGC395770.3824913167019791No Hit
TGAGGGGCAGAGAGCGAGACT395580.3823076914899282No Hit
TAGCTTATCAGACTGATGTTGA378460.36576209343565963No Hit
TCCTGTACTGAGCTGCCCCGAGT370840.3583977559839349No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT363010.3508304643504698No Hit
TGAGGTAGTAGTTTGTACAGT356580.34461620059527426No Hit
TGAGGTAGTAGATTGTATAGT341130.32968457151008446No Hit
TTCAAGTAATCCAGGATAGGCC338110.32676589708695997No Hit
TGAGAACTGAATTCCATGGGTT337200.3258864289660847No Hit
CCCATAAAGTAGAAAGCACT335820.3245527300575046No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT329480.3184254466659122No Hit
TCGTACCGTGAGTAATAATGCG316520.30590027430707334No Hit
TGTAAACATCCCCGACTGGAAGCT308840.29847794994627996No Hit
CGCGACCTCAGATC307510.2971925734619238No Hit
AAAAGCTGGGTTGAGAGGGCGA295840.285914119648062No Hit
TGAGGTAGTAGGTTGTGTGGTT277040.2677448881398698No Hit
TGAGGTAGTAGTTTGTACAGTT269300.26026457687000776No Hit
TGAGGTAGTAGTTTGTGCTGT248170.23984352039298112No Hit
TGAGGTAGTAGGTTGTGTGGT244850.23663491142451318No Hit
CATTGCACTTGTCTCGGTCTG239210.23118414197205556No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT239170.2311454840326764No Hit
TCAGTGCACTACAGAACTTTGC236850.22890332354868673No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG234740.22686411724643749No Hit
CTAGACTGAAGCTCCTTGAGG234210.226351899549664No Hit
AAACCGTTACCATTACTGAGTT232630.2248249109441883No Hit
TAGCTTATCAGACTGATGTTG224330.2168033885230183No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT223340.21584660452338483No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC215520.20828897737476446No Hit
TCCTGTACTGAGCTGCCCCGAA214570.20737085131451008No Hit
TTCAAGTAATCCAGGATAGGCG213600.2064333962845661No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT209370.2023453191952229No Hit
AAACCGTTACCATTACTGAGA201020.19427547434982903No Hit
TGTAAACATCCCCGACTGGAAG200620.1938888949560377No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA200440.19371493422883163No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC189700.18333527750553463No Hit
AAACCGTTACCATTACTG183270.17712101375033912No Hit
TGAGGTAGTAGGTTGTATAG182170.17605792041741297No Hit
TATTGCACTTGTCCCGGCCTGG180770.17470489253914334No Hit
CAACGGAATCCCAAAAGCAGCTG173310.16749518684493517No Hit
TGTAAACATCCTTGACTGGAAGCT166130.16055608672638094No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA164440.1589227887876126No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT164200.15869084115133783No Hit
GCATTGGTGGTTCAGTGGTAGAATT162330.15688358248536338No Hit
TTCAAGTAATCCAGGATAGGCA158100.15279550539602013No Hit
TAGCTTATCAGACTGATGTTGAT157350.15207066903266142No Hit
TATTGCACTTGTCCCGGCCTG153490.14834017788257517No Hit
TGGAGAGAAAGGCAGTTCCTGA150710.14565345109572547No Hit
AGCTCGGTCTGAGGCCCCTCAGT149640.14461935121733369No Hit
TGGCTCAGTTCAGCAGGAAC144600.139748450855563No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT141580.13682977643243854No Hit
TGAGGGGCAGAGAGCGAGACTT141070.1363368877053546No Hit
AAGCTGCCAGTTGAAGAACTGC134750.1302289332834517No Hit
TCAGTGCATCACAGAACTTTGT133690.1292044978899047No Hit
TCCTGTACTGAGCTGCCCCGG130730.1263438103758489No Hit
TGAGGTAGTAGGTTGTATAGTC129230.12489413764913146No Hit
TCCTGTACTGAGCTGCCCCA129100.12476849934614928No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT125770.12155022589283651No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG125100.12090270540823604No Hit
AAAAGCTGGGTTGAGAGGGCG121350.1172785235914424No Hit
TACAGTACTGTGATAACTGAAG120710.1166599965613763No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT118400.1144275005622314No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC114130.11030076553350905No Hit
TCAGTGCACTACAGAACTTTGG113920.1100978113517686No Hit
TAGCAGCACGTAAATATTGGCG111620.1078749798374685No Hit
CAACGGAATCCCAAAAGCAGCT111250.10751739389821152No Hit
TGGAGAGAAAGGCAGTTCCTG110870.10715014347410975No Hit
TGAGGTAGTAGATTGTATAG109560.10588409595944318No Hit
TGAGGTAGTAGGTTGTGTGG107900.1042797914752092No Hit
TTCAAGTAATTCAGGATAGGT105050.10152541329444603No Hit
TGGCTCAGTTCAGCAGGAACAG104940.10141910396115342No Hit
AGAGGTAGTAGGTTGCATAGTT104280.10078124796139773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)