FastQCFastQC Report
Wed 8 May 2024
EGAF00002167585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11048236
Total Bases257.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT130687411.828802353606495No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2806042.539808164850932No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2430222.1996452646377214No Hit
TCCTGTACTGAGCTGCCCCGAG2265342.0504087711377634No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2236622.024413671105505No Hit
AAACCGTTACCATTACTGAGC2227842.0164667011095707No Hit
TATTGCACTTGTCCCGGCCTGT1801661.630721863653166No Hit
TCCTGTACTGAGCTGCCCCGA1764311.596915561905086No Hit
TTCAAGTAATCCAGGATAGGCT1537881.3919688174655211No Hit
TCAGTGCACTACAGAACTTTGT1288271.1660413481391962No Hit
TGAGGTAGTAGGTTGTATAGT1237351.1199525426502477No Hit
TGAGGTAGTAGGTTGTATAGTT1148451.0394872086367453No Hit
TGAGGTAGTAGTTTGTGCTGTT928280.8402065270872202No Hit
TGAGGTAGTAGATTGTATAGTT899510.8141661709615906No Hit
AAGCTGCCAGTTGAAGAACTGT851280.7705121432959977No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT831100.7522467840114929No Hit
TGAGGTAGTAGATTGTATAGT805740.7292928934537604No Hit
TGAGGGGCAGAGAGCGAGACTTT781040.7069363833285242No Hit
AAACCGTTACCATTACTGAG705580.6386358872131261No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC673460.6095633728316447No Hit
TAGCTTATCAGACTGATGTTGAC661430.5986747567666006No Hit
TCCTGTACTGAGCTGCCCCGAGA660660.5979778129286883No Hit
TGAGGGGCAGAGAGCGAGACT647580.5861388189028548No Hit
TGAGGTAGTAGGTTGTGTGGT602070.54494672271664No Hit
TGAGGTAGTAGGTTGTGTGGTT588240.5324288872902425No Hit
CATTGCACTTGTCTCGGTCTGA586320.530691053304799No Hit
TGAGGTAGTAGTTTGTACAGT557420.5045330313364053No Hit
TCCTGTACTGAGCTGCCCCG530700.48034817503898364No Hit
TCCTGTACTGAGCTGCCCCGAGT517780.46865400051193695No Hit
AAAAGCTGGGTTGAGAGGGCGA465620.42144284390738934No Hit
CGCGACCTCAGATCAGA463380.41941537092437203No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC448230.4057027746329821No Hit
TATTGCACTTGTCCCGGCCTGC413570.37433125070825785No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC403900.36557872225032123No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG385670.3490783506072825No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT383910.34748533612062593No Hit
TAGCTTATCAGACTGATGTTGA369250.33421624954427115No Hit
CCCATAAAGTAGAAAGCACT359650.3255270796170538No Hit
TGAGAACTGAATTCCATGGGTT347260.3143126196797389No Hit
TGTAAACATCCCCGACTGGAAGCT344300.31163345895218025No Hit
TTCAAGTAATCCAGGATAGGCC332980.30138747941300315No Hit
TCGTACCGTGAGTAATAATGCG319320.2890235147040668No Hit
AAACCGTTACCATTACTGAGTT312720.28304971037910487No Hit
TGAGGTAGTAGTTTGTGCTGT306500.277419852363762No Hit
TGAGGTAGTAGTTTGTACAGTT286950.2597247198557308No Hit
TGAGGTAGTAGGTTGTATAG266490.24120592644834887No Hit
TGGAGTGTGACAATGGTGTTT263070.23811040966177768No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT260470.23575709280648965No Hit
CGCGACCTCAGATCAGACGT249140.22550206204863835No Hit
CATTGCACTTGTCTCGGTCTG239190.21649609946782455No Hit
TAGCTTATCAGACTGATGTTG233150.21102916338861694No Hit
TCAGTGCACTACAGAACTTTGC233150.21102916338861694No Hit
TGGAGAGAAAGGCAGTTCCTGA221130.20014959854224693No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC217920.19724415734783363No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT214980.19458309905762333No Hit
TGAGGTAGTAGGTTGTATAGC214360.19402192349982386No Hit
TGGCTCAGTTCAGCAGGAAC212760.1925737285119543No Hit
TCCTGTACTGAGCTGCCCCGAA211970.1918586822366937No Hit
CGCGACCTCAGATC211790.19169576030055838No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT211510.19144232617768123No Hit
TGTAAACATCCTTGACTGGAAGCT207690.18798476064414263No Hit
TGAGGTAGTAGGTTGTATAGTC206120.18656371931229565No Hit
TGAGGGGCAGAGAGCGAGACTT201850.18269884893841876No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT200850.1817937270710003No Hit
AAACCGTTACCATTACTG194830.1763448934291411No Hit
CTAGACTGAAGCTCCTTGAGG194510.1760552544315672No Hit
CAACGGAATCCCAAAAGCAGCT189410.17143913290773297No Hit
TGTAAACATCCCCGACTGGAAG188820.17090511100595607No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT183410.16600840170322212No Hit
AGAGGTAGTAGGTTGCATAGTT176830.16005269981560857No Hit
AGCTCGGTCTGAGGCCCCTCAGT175390.15874932432652597No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA173460.15700243912240833No Hit
TATTGCACTTGTCCCGGCCTG171050.1548210954219298No Hit
AGAGGTAGTAGGTTGCATAGT168700.1526940590334964No Hit
TGAGGTAGTAGGTTGTGTGG167300.15142688841911053No Hit
TGAGGTAGTAGTTTGTGCTGTC166200.1504312543649502No Hit
TTCAAGTAATTCAGGATAGGT165730.15000584708726353No Hit
AAGCTGCCAGTTGAAGAACTGC162600.14717281564224372No Hit
TGAGGTAGTAGATTGTATAGTC160300.1450910353471812No Hit
CAACGGAATCCCAAAAGCAGCTG158110.14310881845753476No Hit
TACAGTATAGATGATGTACT148700.13459162168512692No Hit
AAAAGCTGGGTTGAGAGGGCG147820.13379511444179867No Hit
AAACCGTTACCATTACTGAGA146690.1327723267316158No Hit
TGGAGAGAAAGGCAGTTCCTG145560.13174953902143294No Hit
AAAAGCTGGGTTGAGAGGGCGT141870.12840963933065877No Hit
TGAGGTAGTAGATTGTATAGC138060.12496112501579437No Hit
TGAGGTAGTAGATTGTATAG137630.12457192261280445No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT135480.1226259105978547No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA134750.12196517163463924No Hit
TACAGTACTGTGATAACTGAAG131730.11923170359503545No Hit
GCATTGGTGGTTCAGTGGTAGAATT130120.11777445738849171No Hit
AAGCTGCCAGTTGAAGAACT129540.11724948670538898No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC128210.11604567462172241No Hit
TCCTGTACTGAGCTGCCCCA126120.11415396991881781No Hit
TGAGGTAGTAGGTTGTGTGGC124050.11228036765326156No Hit
TGAGGGGCAGAGAGCGAGACC120370.10894951918116158No Hit
AAAAGCTGGGTTGAGAGGGCGAA119250.10793578268965291No Hit
TTCAAGTAATCCAGGATAGGCA119170.10786337294025941No Hit
TGAGGTAGTAGTTTGTGCTG117140.10602597554939992No Hit
TAGCAGCACGTAAATATTGGCG116840.10575443898917437No Hit
TGAGGTAGTAGGTTGTGTGGTC115670.10469544640429476No Hit
TGAGGTAGTAGGTTGTAT113940.10312958557366081No Hit
TCAGTGCATCACAGAACTTTGT113310.10255935879718717No Hit
AAAAGCTGGGTTGAGAGGGCGAT113060.10233307833033255No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT111710.1011111638093176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)