FastQCFastQC Report
Wed 8 May 2024
EGAF00002167587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15053591
Total Bases336.4 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11600757.706300775675386No Hit
TCAGTGCACTACAGAACTTTGT5793523.8485966571032786No Hit
CGCGACCTCAGATCAGA2841461.8875629077473939No Hit
TTCAAGTAATCCAGGATAGGCT2837391.8848592339196675No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2673131.7757424125579073No Hit
TAGCTTATCAGACTGATGTTGAC2017141.3399726351008208No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1840711.2227713639888318No Hit
TGAGGGGCAGAGAGCGAGACTTT1772841.1776857761048511No Hit
TATTGCACTTGTCCCGGCCTGT1670181.1094894234870603No Hit
AAGCTGCCAGTTGAAGAACTGT1654661.0991795911022162No Hit
AAACCGTTACCATTACTGAGC1427880.948531151138622No Hit
TCCTGTACTGAGCTGCCCCGAG1269930.8436060206498237No Hit
TGAGGTAGTAGGTTGTATAGTT1170480.777542049601321No Hit
TCCTGTACTGAGCTGCCCCGA1149720.7637513201999444No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1109980.7373523035134939No Hit
TAGCTTATCAGACTGATGTTGA982390.6525951183342235No Hit
TGAGGTAGTAGGTTGTATAGT960820.6382663113405964No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT952210.6325467458229734No Hit
TGGCTCAGTTCAGCAGGAAC940020.6244490102062691No Hit
TGAGGTAGTAGATTGTATAGTT882910.5865112184860077No Hit
TGAGGGGCAGAGAGCGAGACT840060.5580462495626459No Hit
TGGAGTGTGACAATGGTGTTT831630.5524462568433007No Hit
TGAGGTAGTAGTTTGTGCTGTT791800.5259874537577114No Hit
TCAGTGCACTACAGAACTTTGC761520.5058726519140848No Hit
TCGTACCGTGAGTAATAATGCG703150.4670978506058787No Hit
CCCATAAAGTAGAAAGCACT700520.4653507591643748No Hit
CATTGCACTTGTCTCGGTCTGA668750.4442461602683373No Hit
CGCGACCTCAGATCAGACGT653370.4340293289488203No Hit
TGAGGTAGTAGATTGTATAGT639080.4245365773522079No Hit
AAACCGTTACCATTACTGAG628090.4172359937240224No Hit
TGAGAACTGAATTCCATGGGTT618270.41071263328464286No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG574250.3814704411724751No Hit
TTCACAGTGGCTAAGTTCCG562300.3735321359534745No Hit
TAGCTTATCAGACTGATGTTG532660.353842481837058No Hit
TGAGGTAGTAGTTTGTACAGT523240.34758483872718476No Hit
TGAGGTAGTAGGTTGTGTGGTT522000.34676111500571527No Hit
TGTAAACATCCCCGACTGGAAGCT500500.33247880854475187No Hit
CGCGACCTCAGATC488730.32466007612402914No Hit
TGAGGTAGTAGGTTGTGTGGT475960.3161770503795407No Hit
TTCAAGTAATCCAGGATAGGCC452250.30042665567305504No Hit
AAAAGCTGGGTTGAGAGGGCGA445630.2960290338697258No Hit
CGGACAGGATTGACAGA441550.2933187171087616No Hit
TGTAAACATCCTTGACTGGAAGCT403760.2682150724036544No Hit
CTAGACTGAAGCTCCTTGAGG390050.25910761093482615No Hit
TGAGATGAAGCACTGTAGCTC372120.24719683163970646No Hit
TCCTGTACTGAGCTGCCCCGAGT364220.24194891438195712No Hit
TCCTGTACTGAGCTGCCCCG362300.2406734712003269No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC342840.2277463231198456No Hit
TGTAAACATCCCCGACTGGAAG337460.22417242503798596No Hit
TGAGGGGCAGAGAGCGAGACTT335950.2231693421190997No Hit
CGCGACCTCAGATCAGAC326290.21675226861152266No Hit
TCCTGTACTGAGCTGCCCCGAGA316670.21036176683689625No Hit
TGGCTCAGTTCAGCAGGAACAG307600.204336626390341No Hit
TATTGCACTTGTCCCGGCCTGC291330.19352857401267246No Hit
AGCTCGGTCTGAGGCCCCTCAGT286710.19045953885687475No Hit
TCAGTGCATCACAGAACTTTGT282680.18778243676209883No Hit
TGAGGTAGTAGTTTGTACAGTT281950.18729750263574982No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT281770.187177929837472No Hit
AAACCGTTACCATTACTGAGTT279970.18598220185469366No Hit
TGTAAACATCCCCGACTGGAAGC272820.18123250458976864No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC272490.18101328779292597No Hit
GGCTGGTCCGATGGTAGTGGGT263140.1748021452157163No Hit
TGAGATGAAGCACTGTAGCT261380.1736329889658886No Hit
CATTGCACTTGTCTCGGTCTG237800.1579689523914925No Hit
TAGCTTATCAGACTGATGTTGACT237510.15777630732760045No Hit
GGCTGGTCCGATGGTAGTGGGTT235850.1566735804101493No Hit
TTCAAGTAATTCAGGATAGGT231970.15409612231393824No Hit
AAGCTGCCAGTTGAAGAACTGC227190.15092080022633803No Hit
TGAGGTAGTAGTTTGTGCTGT226750.1506285111638811No Hit
AAGCTGCCAGTTGAAGAACT217450.14445058325285973No Hit
TGAGGTAGTAGGTTGTGTGG214120.14223848648471982No Hit
GGCTGGTCCGATGGTAGT210980.1401526054480954No Hit
AACCCGTAGATCCGATCTTGT208870.1387509465349497No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC208330.1383922281401162No Hit
TGGGTGTCAGAGCAAACCAGGACGT208170.1382859412083137No Hit
GCATTGGTGGTTCAGTGGTAGAATT204100.13558226738058712No Hit
CAACGGAATCCCAAAAGCAGCTG203640.13527669245165488No Hit
TGAGGTAGTAGGTTGTATAG203510.13519033431956534No Hit
AAACCGTTACCATTACTG195400.12980291546382522No Hit
TACAGTACTGTGATAACTGAAG187650.12465464220464074No Hit
GGCTGGTCCGATGGTAGTGGGTTATC176860.11748691724120842No Hit
AAAAGCTGGGTTGAGAGGGCGT176120.11699534018162178No Hit
CAACGGAATCCCAAAAGCAGCT169810.11280364930865998No Hit
AAAAGCTGGGTTGAGAGGGCG166990.1109303421356406No Hit
GGCTGGTCCGATGGTAGTG165220.10975454295257524No Hit
TGAAAAGAACTTTGAAGAGAG163180.10839938457209314No Hit
TGAGAACTGAATTCCATGGGT161290.10714387019017588No Hit
AACATTCAACGCTGTCGGTGA153990.102294528926686No Hit
CGCGACCTCAGATCAGACG153090.10169666493529683No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)