FastQCFastQC Report
Wed 8 May 2024
EGAF00002167588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13505054
Total Bases299.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10226877.572624293097976No Hit
TCAGTGCACTACAGAACTTTGT6715284.9724199547813726No Hit
CGCGACCTCAGATCAGA2713092.008944207109427No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1991061.474307322280977No Hit
TTCAAGTAATCCAGGATAGGCT1908061.4128488490308888No Hit
TCCTGTACTGAGCTGCCCCGAG1460971.0817950080021894No Hit
TATTGCACTTGTCCCGGCCTGT1459511.0807139312438143No Hit
TGAGGGGCAGAGAGCGAGACTTT1409031.0433353320912304No Hit
TGGAGTGTGACAATGGTGTTT1355501.003698319162589No Hit
TGAGGTAGTAGGTTGTATAGTT1353121.001936015953731No Hit
TGAGGTAGTAGGTTGTATAGT1301990.9640761155046104No Hit
AAGCTGCCAGTTGAAGAACTGT1255900.929948151262483No Hit
CATTGCACTTGTCTCGGTCTGA1226140.9079119565164271No Hit
TCCTGTACTGAGCTGCCCCGA1091270.8080456398026992No Hit
CGCGACCTCAGATCAGACGT1009530.747520150604359No Hit
TGGCTCAGTTCAGCAGGAAC992420.7348508195524431No Hit
AAACCGTTACCATTACTGAGC950490.7038031836081514No Hit
TAGCTTATCAGACTGATGTTGAC925460.6852693813738175No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT897850.664825183223999No Hit
TGAGGGGCAGAGAGCGAGACT893310.6614634787835724No Hit
TCGTACCGTGAGTAATAATGCG877020.6494013278288261No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT810160.5998939360035139No Hit
TGAGGTAGTAGATTGTATAGTT805940.5967691798936902No Hit
TGAGGTAGTAGGTTGTGTGGTT789930.5849143587282213No Hit
TGAGGTAGTAGGTTGTGTGGT769220.5695793589570245No Hit
TGAGGTAGTAGATTGTATAGT754900.5589759211625515No Hit
TAGCTTATCAGACTGATGTTGA695370.5148961270351085No Hit
TTCACAGTGGCTAAGTTCCG680080.5035744396134958No Hit
TCAGTGCACTACAGAACTTTGC653250.48370780301952143No Hit
CCCATAAAGTAGAAAGCACT623940.4620048168633757No Hit
TGGCTCAGTTCAGCAGGAACAG620980.45981304480530033No Hit
TGAGGTAGTAGTTTGTGCTGTT616360.45639210328222307No Hit
AAATGGATTTTTGGAGCAGGGAGA612340.45341543987902605No Hit
AAAAGCTGGGTTGAGAGGGCGA591260.4378064686005699No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG530510.3928233089627039No Hit
AAACCGTTACCATTACTGAG515750.38189406721365204No Hit
AAATGGATTTTTGGAGCAGGGA505970.37465233385960545No Hit
TGAGGTAGTAGTTTGTACAGT472150.34960985716902726No Hit
AAATGGATTTTTGGAGCAGGGAG444940.32946184443246207No Hit
TGAGATGAAGCACTGTAGCTC438850.3249524215156785No Hit
TAGCTTATCAGACTGATGTTG427720.31671106239190155No Hit
CTAGACTGAAGCTCCTTGAGG423100.31329012086882435No Hit
TCCTGTACTGAGCTGCCCCGAGA417560.30918795289526424No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC416960.3087436747753841No Hit
CGCGACCTCAGATC408150.30222019104847714No Hit
TGAGAACTGAATTCCATGGGTT401960.29763672177838013No Hit
TGAAAAGAACTTTGAAGAGAG385820.28568564035360394No Hit
AAATGGATTTTTGGAGCAGGG382740.28340501267155244No Hit
CATTGCACTTGTCTCGGTCTG358130.26518220512113466No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC354480.262479513225197No Hit
CGTGTATTTGACAAGCTGAGTTG352870.26128736693685195No Hit
TCCTGTACTGAGCTGCCCCG350600.25960651471663865No Hit
TGAGGTAGTAGGTTGTATAG349670.2589178836308244No Hit
TCCTGTACTGAGCTGCCCCGAGT340220.251920503242712No Hit
TGAGGTAGTAGGTTGTGTGG336350.24905490936948496No Hit
TGTAAACATCCCCGACTGGAAG327720.242664709078542No Hit
TGAGGGGCAGAGAGCGAGACTT326320.24162806013215496No Hit
TGAGGTAGTAGTTTGTACAGTT321950.23839223449236116No Hit
TGGCTCAGTTCAGCAGGAACA316380.23426785261280703No Hit
TGAGATGAAGCACTGTAGCT284480.21064706590584534No Hit
TGTAAACATCCTTGACTGGAAGCT254050.18811476059259No Hit
GTTGAAAAGAACTTTGAAGAGAG250980.18584153754586985No Hit
AAACCGTTACCATTACTGAGTT250700.18563420775659245No Hit
TGTAAACATCCCCGACTGGAAGCT248510.18401259261902989No Hit
AAATGGATTTTTGGAGCAGGGAGAT241210.17860720882715464No Hit
TTCAAGTAATTCAGGATAGGT235500.1743791620529618No Hit
TTCAAGTAATCCAGGATAGGCC232100.17186158604030757No Hit
TGTAAACATCCCCGACTGGAAGC221040.16367205936385001No Hit
AAACCGTTACCATTACTG217990.16141364558779253No Hit
CGCGACCTCAGATCAGAC217780.16125814824583448No Hit
AAAAGCTGGGTTGAGAGGGCG211990.15697086438899097No Hit
CGCGACCTCAGATCAGACG209780.1553344399807657No Hit
GGCTGGTCCGATGGTAGTGGGT208090.15408305660976995No Hit
GGCTGGTCCGATGGTAGTGGGTTATC207450.15360915994856444No Hit
AGCTCGGTCTGAGGCCCCTCAGT205850.1524244182955507No Hit
AAATGGATTTTTGGAAATAGGAGA202860.15021043233148124No Hit
TCAGTGCATCACAGAACTTTGT189710.14047333687077446No Hit
GGCTGGTCCGATGGTAGTGGGTT186720.138259350906705No Hit
TGGACGGTGTGAGGCC185500.137355985396282No Hit
TATTGCACTTGTCCCGGCCTGC183400.13580101197670147No Hit
TGAGGTAGTAGTTTGTGCTGT180870.13392763923787346No Hit
GGCTGGTCCGATGGTAGTG179900.1332093896107339No Hit
GGCTGGTCCGATGGTAGT178380.1320838850403708No Hit
AACATTCAACGCTGTCGGTGA174250.12902577064852905No Hit
AAAAGCTGGGTTGAGAGGGCGT173020.12811500050277475No Hit
TAGCAGCACGTAAATATTGGCG172400.12765591311223193No Hit
AACCCGTAGATCCGATCTTGT171620.12707835155638772No Hit
AGGAAACTCTGGTG170560.1262934602112661No Hit
CAACGGAATCCCAAAAGCAGCTG170290.12609353505732002No Hit
AAGTTGAAAAGAACTTTGAAGAGAG168520.12478291460367356No Hit
AGTTGAAAAGAACTTTGAAGAGAG162670.12045120293484202No Hit
AAGCTGCCAGTTGAAGAACT159110.11781515275688642No Hit
TGAGGTAGTAGATTGTATAG154180.1141646675385378No Hit
TGTCAGTTTGTCAAATACCCCAA150120.1111583855940154No Hit
ACGGCCCTGGCGGAGC149310.11055861013217719No Hit
TGGAGAGAAAGGCAGTTCCTGA145840.10798920167220362No Hit
TCGTACCGTGAGTAATAATGC145580.10779668115358887No Hit
AAAAGCTGGGTTGAGAGGGCGAT145430.10768561162361884No Hit
AGAGGTAGTAGGTTGCATAGTT144130.10672300903054517No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA143080.10594552232075488No Hit
TTCACAGTGGCTAAGTTCCGA142080.10520505878762129No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC141730.10494589655102451No Hit
CTAGACTGAAGCTCCTTGAGGA139230.10309473771819054No Hit
TATTGCACTTGTCCCGGCCTG137520.10182854507653209No Hit
CAACGGAATCCCAAAAGCAGCT136180.10083632394213309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)