FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11605621
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT163155814.058342935720544No Hit
AAACCGTTACCATTACTGAGC2806072.417854244938724No Hit
TCAGTGCACTACAGAACTTTGT2742652.3632083108693624No Hit
TATTGCACTTGTCCCGGCCTGT2142231.8458555556828886No Hit
TTCAAGTAATCCAGGATAGGCT1932691.6653051137892578No Hit
CGCGACCTCAGATCAGA1880831.6206198703197356No Hit
TGGAGTGTGACAATGGTGTTT1841071.5863606092254778No Hit
TCCTGTACTGAGCTGCCCCGA1563751.3474074330016463No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1456611.2550900981515767No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1308131.1271520929384131No Hit
AAGCTGCCAGTTGAAGAACTGT1281501.1042063151984713No Hit
TGAGGGGCAGAGAGCGAGACTTT1203291.0368165563910798No Hit
TCCTGTACTGAGCTGCCCCGAG1192751.027734750255932No Hit
TGAGGGGCAGAGAGCGAGACT965750.8321398742902254No Hit
TGAGGTAGTAGGTTGTATAGTT962270.8291413272930419No Hit
TAGCTTATCAGACTGATGTTGAC881910.7598990178983098No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG777760.6701580208417972No Hit
TGAGGTAGTAGGTTGTATAGT754420.6500470763262043No Hit
TGAGGTAGTAGATTGTATAGTT733710.6322022750872185No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC591280.5094772610616873No Hit
CATTGCACTTGTCTCGGTCTGA567660.48912505414402213No Hit
TGAGGTAGTAGGTTGTGTGGTT549350.4733482163513697No Hit
AAACCGTTACCATTACTGAG548240.4723917832574405No Hit
TAGCTTATCAGACTGATGTTGA542840.4677388655031902No Hit
TGAGGTAGTAGTTTGTGCTGTT541660.4667221168087429No Hit
TGAGGTAGTAGGTTGTGTGGT524670.452082658911574No Hit
CGCGACCTCAGATCAGACGT518760.4469902989249778No Hit
CCCATAAAGTAGAAAGCACT514510.4433282803220956No Hit
TGAGGTAGTAGATTGTATAGT510480.4398558250351274No Hit
TCAGTGCACTACAGAACTTTGC500530.43128239324720324No Hit
TATTGCACTTGTCCCGGCCTGC499790.4306447711845837No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT486110.41885737954048297No Hit
TCGTACCGTGAGTAATAATGCG471010.4058464428573017No Hit
TGAGAACTGAATTCCATGGGTT430720.37113050650197865No Hit
CGCGACCTCAGATC427240.3681319595047951No Hit
TTCAAGTAATCCAGGATAGGCC426190.36722722549702425No Hit
TGAGGTAGTAGTTTGTACAGT422640.36416836289932264No Hit
TGGCTCAGTTCAGCAGGAAC415220.3577749092444084No Hit
TCCTGTACTGAGCTGCCCCG414160.35686155872227776No Hit
TGTAAACATCCCCGACTGGAAGCT397290.3423254989974255No Hit
TAGCTTATCAGACTGATGTTG368250.31730314129679055No Hit
TCCTGTACTGAGCTGCCCCGAGT351180.30259475128474383No Hit
AAAAGCTGGGTTGAGAGGGCGA324970.2800108671479105No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT310990.26796498007301806No Hit
TCCTGTACTGAGCTGCCCCGAGA295710.25479894613136167No Hit
TGGAGTGTGACAATGGTGTTC290650.25043898986534197No Hit
TGAGGGGCAGAGAGCGAGACTT288240.24836240990464878No Hit
AACCCGTAGATCCGATCTTGT272330.23465353555832988No Hit
AAGCTGCCAGTTGAAGAACT271440.23388666578031456No Hit
TGAGGTAGTAGTTTGTGCTGT246860.212707273484116No Hit
CTAGACTGAAGCTCCTTGAGG246490.2123884624528063No Hit
AAGCTGCCAGTTGAAGAACTGC243140.20950193014229918No Hit
AAACCGTTACCATTACTG242180.20867474476376577No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC238770.20573651336710028No Hit
TGTAAACATCCTTGACTGGAAGCT229850.1980505825582276No Hit
TGAGGTAGTAGGTTGTGTGG228090.1965340760309164No Hit
TGAGGTAGTAGTTTGTACAGTT224600.19352691251937315No Hit
TTCACAGTGGCTAAGTTCCG224530.1934665969188551No Hit
AGCTCGGTCTGAGGCCCCTCAGT205500.1770693700923027No Hit
AAACCGTTACCATTACTGAGTT204760.1764317480296832No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC204530.17623356819940958No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCA201840.17391572583664416No Hit
CGCGACCTCAGATCAGAC193720.16691911617654928No Hit
AAACCGTTACCATTACTGAGA193090.16637627577188674No Hit
TGTAAACATCCCCGACTGGAAG191570.16506656558920887No Hit
TATTGCACTTGTCCCGGCCTG190580.16421353066759634No Hit
CATTGCACTTGTCTCGGTCTG186560.16074969189498778No Hit
TGAGGGGCAGAGAGCGAGACC185700.16000867166005162No Hit
TTCAAGTAATTCAGGATAGGT181430.15632942002845002No Hit
TACCCTGTAGAACCGAATTTGT177650.1530723776004748No Hit
TGAGGTAGTAGGTTGTATAG177360.15282249868404285No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC176980.15249507113837338No Hit
TCAGTGCATCACAGAACTTTGT172190.14836776076006616No Hit
TGAGGTAGTAGGTTGTATAGTC171330.14762674052513003No Hit
TGAGATGAAGCACTGTAGCTC169150.14574834039471046No Hit
TGTAAACATCCCCGACTGGAAGC168780.14542952936340073No Hit
TAGCTTATCAGACTGATGTTGAT165350.1424740649380158No Hit
TGAGGGGCAGAGAGCGAGACTTC163370.1407679950947907No Hit
TGGCTCAGTTCAGCAGGAACAG157360.1355894699645973No Hit
ACCAATATTACTGTGCTGCTT154180.1328494183982055No Hit
AAAAGCTGGGTTGAGAGGGCGT148310.12779152446904823No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG143290.1234660342604674No Hit
TGAGATGAAGCACTGTAGCT140130.120743215722795No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC139430.12014005971761443No Hit
TGAGAACTGAATTCCATGGGT137120.11814964490051846No Hit
AGACCGTTACCATTACTGAGT135320.11659867231576836No Hit
TAGCTTATCAGACTGATGTTGACT134920.11625401174137945No Hit
TTCAAGTAATCCAGGATAGGCA132290.11398786846477237No Hit
TCCTGTACTGAGCTGCCCCGAA131370.11319514914367788No Hit
TGAGGTAGTAGGTTGTATAGC129360.1114632297573736No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT128940.11110133615426525No Hit
CAACGGAATCCCAAAAGCAGCT128360.11060157832140133No Hit
TGAGGTAGTAGATTGTATAGTC126010.10857669744686647No Hit
AAACCGTTACCATTACTGAGG123200.1061554569117844No Hit
CATTGCACTTGTCTCGGTCT123180.10613822388306493No Hit
GGAATACCGGGTGCTGTAGGCTT120480.10381176500593979No Hit
AAAAGCTGGGTTGAGAGGGCG119930.10333785671615506No Hit
AAACCGTTACTATTACTGAGT118230.10187304927500218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)