FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12383017
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11451129.247439456797968No Hit
TCAGTGCACTACAGAACTTTGT5038184.068620756960925No Hit
TTCAAGTAATCCAGGATAGGCT2121771.713451576461536No Hit
TGAGGTAGTAGGTTGTATAGT1993811.6101165006879987No Hit
TGAGGTAGTAGGTTGTGTGGT1897451.5323002463777606No Hit
AAACCGTTACCATTACTGAGC1670461.348992737391865No Hit
TGAGGTAGTAGGTTGTATAGTT1552151.253450592856329No Hit
TATTGCACTTGTCCCGGCCTGT1492471.205255552826908No Hit
TGAGGTAGTAGGTTGTGTGGTT1466801.1845255481761836No Hit
TCCTGTACTGAGCTGCCCCGAG1410711.1392296400788273No Hit
CGCGACCTCAGATCAGA1210850.9778311698998718No Hit
TCCTGTACTGAGCTGCCCCGA1184430.9564954970182146No Hit
TGAGGTAGTAGATTGTATAGT1097510.8863025868413166No Hit
AAGCTGCCAGTTGAAGAACTGT1074720.8678983481973738No Hit
TGAGGTAGTAGATTGTATAGTT1073120.866606255971384No Hit
CGCGACCTCAGATCAGACGT1072140.8658148494829652No Hit
TGGAGTGTGACAATGGTGTTT1032890.834118212064152No Hit
TGAGGGGCAGAGAGCGAGACTTT875690.7071701508606506No Hit
TGAGGGGCAGAGAGCGAGACT802110.6477500596179429No Hit
TCAGTGCACTACAGAACTTTGC760160.6138730165677718No Hit
CCCATAAAGTAGAAAGCACT736890.5950811502560321No Hit
TCGTACCGTGAGTAATAATGCG700770.5659121682543116No Hit
CATTGCACTTGTCTCGGTCTGA656760.5303715564631786No Hit
TGGCTCAGTTCAGCAGGAAC642280.5186781218179705No Hit
TAGCTTATCAGACTGATGTTGAC589720.4762328921942044No Hit
TTCACAGTGGCTAAGTTCCG579190.4677293102319087No Hit
TGAGGTAGTAGTTTGTACAGT557530.4502376117225713No Hit
TAGCTTATCAGACTGATGTTGA518860.41900935773567943No Hit
TGAGGTAGTAGTTTGTGCTGTT514940.4158437317820044No Hit
CTAGACTGAAGCTCCTTGAGG513080.41434167456929116No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC511710.4132353206007873No Hit
TCCTGTACTGAGCTGCCCCGAGA511020.41267810582832926No Hit
TGAGGTAGTAGGTTGTGTGG460550.37192067167476234No Hit
TCCTGTACTGAGCTGCCCCGAGT453860.3665181110548423No Hit
TGGCTCAGTTCAGCAGGAACAG448320.36204424172235244No Hit
AAAAGCTGGGTTGAGAGGGCGA433320.34993087710369775No Hit
TGAGGTAGTAGGTTGTATAG416600.336428513342104No Hit
TTCAAGTAATCCAGGATAGGCC405350.3273434898781129No Hit
TGAGATGAAGCACTGTAGCT393910.31810503046228555No Hit
AAACCGTTACCATTACTGAG388170.313469649601547No Hit
TTCAAGTAATTCAGGATAGGT343730.2775817880246793No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC340000.2745695980228405No Hit
TCCTGTACTGAGCTGCCCCG328730.26546842340602456No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT328590.2653553653362505No Hit
TGAGGTAGTAGGTTGTGTGGC323620.26134180385926953No Hit
TAGCTTATCAGACTGATGTTG307530.2483482014116592No Hit
TGAGGTAGTAGTTTGTACAGTT304770.24611934232182672No Hit
TGGCTCAGTTCAGCAGGAACA297410.24017571808227348No Hit
TGAGGTAGTAGGTTGTATAGC296820.2396992590739397No Hit
TGTAAACATCCCCGACTGGAAGCT292970.2365901621551517No Hit
CGTGTATTTGACAAGCTGAGTTG292010.2358149068195578No Hit
TATTGCACTTGTCCCGGCCTGC284730.229935887191304No Hit
CGCGACCTCAGATC274280.2214969098403079No Hit
TGAGATGAAGCACTGTAGCTC265190.21415621088140313No Hit
AGGAAACTCTGGTG263730.2129771767251874No Hit
TGAGGTAGTAGGTTGTGTGGTC256040.20676705846402374No Hit
AGCTCGGTCTGAGGCCCCTCAGT249820.20174404993548828No Hit
TGAGGTAGTAGGTTGTATAGTC243900.19696330869932588No Hit
TGAGAACTGAATTCCATGGGTT238580.19266710204790966No Hit
TGTAAACATCCCCGACTGGAAG229110.1850195311853323No Hit
TGAGGTAGTAGGTTGTGTGGTTT216190.17458588646046436No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC211540.1708307434286814No Hit
TGAGGGGCAGAGAGCGAGACTT209880.16949019774421695No Hit
TGTAAACATCCTTGACTGGAAGCT203180.1640795615478845No Hit
CATTGCACTTGTCTCGGTCTG200460.16188300476370177No Hit
TGTAAACATCCCCGACTGGAAGC194630.15717494371525131No Hit
TGAGGTAGTAGGTTGTAT189040.1526606965006993No Hit
CGCGACCTCAGATCAGACGC185640.14991500052047088No Hit
AAACCGTTACCATTACTGAGTT185300.14964043092244805No Hit
CAACGGAATCCCAAAAGCAGCT181760.14678167687244553No Hit
CTAGACTGAAGCTCCTTGAGGA181300.14641020035747346No Hit
AGAGGTAGTAGGTTGCATAGT175160.14145179644023748No Hit
AAGCTGCCAGTTGAAGAACTGC173710.14028083786043416No Hit
AAACCGTTACCATTACTG169720.137058682871872No Hit
TGTCAGTTTGTCAAATACCCCA169580.1369456248020979No Hit
TGAGGTAGTAGATTGTATAGTC169050.13651761925223876No Hit
CAACGGAATCCCAAAAGCAGCTG168170.13580696852794436No Hit
AGAGGTAGTAGGTTGCATAGTT165490.1336427140494114No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT164140.13255251123373246No Hit
TGAGGTAGTAGTTTGTGCTGT163080.1316965001340142No Hit
TGAGGTAGTAGATTGTATAGC159550.12884582166042413No Hit
TCAGTGCATCACAGAACTTTGT159200.12856317648598883No Hit
GAAGAGGGGGACGG159170.12853894975675154No Hit
TTCAAGTAATCCAGGATAGGCA158120.1276910142334457No Hit
TGTCAGTTTGTCAAATACCCCAA157930.12753757828160942No Hit
TGAGGTAGTAGATTGTATAG157760.127400293482598No Hit
CGCGACCTCAGATCAGACG157660.1273195377184736No Hit
AAGCTGCCAGTTGAAGAACT150990.12193312825137849No Hit
TGGAGAGAAAGGCAGTTCCTGA148110.11960736224459678No Hit
TGGAGTGTGACAATGGTGTTC141420.11420480162467678No Hit
TCGTACCGTGAGTAATAATGCA138480.11183058215942043No Hit
AAAAGCTGGGTTGAGAGGGCG135310.10927062443667807No Hit
TGAGGTAGTAGATTGTAT135260.1092302465546159No Hit
TGAGGGGCAGAGAGCGAGACC134780.10884261888681893No Hit
GTAGTCGTGGCCGAGTGGT129610.10466754588158927No Hit
TCCTGTACTGAGCTGCCCCGAA128330.10363387210079741No Hit
TGGAGTGTGACAATGGTGTTTG128170.10350466287819841No Hit
AACCCGTAGATCCGATCTTGT126830.10242253563893192No Hit
ACCAATATTACTGTGCTGCTT125510.10135655955249032No Hit
TGTCAGTTTGTCAAATACCCCAT124360.10042786826506012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)