FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007906
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT142312610.940469588264245No Hit
TCAGTGCACTACAGAACTTTGT4024913.0942028640120864No Hit
AAACCGTTACCATTACTGAGC2160581.660974487361763No Hit
TTCAAGTAATCCAGGATAGGCT1722231.323987119833123No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1644011.263854458972874No Hit
TCCTGTACTGAGCTGCCCCGAG1518631.1674669235770923No Hit
TGAGGTAGTAGGTTGTATAGT1471081.1309122313768258No Hit
TATTGCACTTGTCCCGGCCTGT1414391.0873310431363818No Hit
TGAGGTAGTAGGTTGTGTGGT1142740.8784965082004744No Hit
AAGCTGCCAGTTGAAGAACTGT1119850.8608995175703146No Hit
CGCGACCTCAGATCAGA1102240.847361596862708No Hit
TGAGGTAGTAGGTTGTATAGTT1051240.8081546714744096No Hit
TCCTGTACTGAGCTGCCCCGA1029580.7915032596330261No Hit
TGAGGTAGTAGGTTGTGTGGTT999620.7684711128755082No Hit
AAACCGTTACCATTACTGAGG983260.7558941462215364No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT971570.746907303911944No Hit
TGAGGGGCAGAGAGCGAGACTTT947510.7284108602875821No Hit
TGAGGTAGTAGATTGTATAGT853440.656093302027244No Hit
CGCGACCTCAGATCAGACGT771000.5927164602819239No Hit
TGAGGTAGTAGATTGTATAGTT718240.5521565115861077No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT713530.5485356367120119No Hit
CCCATAAAGTAGAAAGCACT691000.5312154008492989No Hit
TGGAGTGTGACAATGGTGTTT675200.5190689416113554No Hit
AAACCGTTACCATTACTGAG657660.5055848343307524No Hit
TCAGTGCACTACAGAACTTTGC626430.4815763582547414No Hit
TGAGGGGCAGAGAGCGAGACT605950.46583208703998935No Hit
TAGCTTATCAGACTGATGTTGAC597400.4592591613131276No Hit
TTCACAGTGGCTAAGTTCCG556000.4274323630567441No Hit
TGAGGTAGTAGTTTGTGCTGTT554750.4264714090031093No Hit
TAGCTTATCAGACTGATGTTGA548330.4215359489836412No Hit
TGGCTCAGTTCAGCAGGAAC532630.40946636606998854No Hit
TGAGGTAGTAGTTTGTACAGT531610.4086822275622225No Hit
TCGTACCGTGAGTAATAATGCG518880.3988958714800061No Hit
TCCTGTACTGAGCTGCCCCGAGA485180.3729885501940128No Hit
TCCTGTACTGAGCTGCCCCGAGT473570.3640632089438531No Hit
CATTGCACTTGTCTCGGTCTGA435030.334435073562186No Hit
TGAGGTAGTAGGTTGTATAG423410.32550204467959715No Hit
CGCGACCTCAGATC423040.3252176022797213No Hit
TCCTGTACTGAGCTGCCCCG392390.3016550088845968No Hit
TAGCTTATCAGACTGATGTTG369930.2843885864488873No Hit
TGAGGTAGTAGGTTGTGTGG361110.27760809464644043No Hit
TTCAAGTAATCCAGGATAGGCC331560.25489114081851455No Hit
TTCAAGTAATTCAGGATAGGT326630.251101138030979No Hit
TGTAAACATCCCCGACTGGAAGCT311600.23954662649007455No Hit
AAACCGTTACCATTACTG306610.2357104979079646No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC296100.22763079622500348No Hit
TCAGTGCACTACAGAACTTTGG286730.22042748463895726No Hit
TGAGATGAAGCACTGTAGCT276820.21280904090174085No Hit
AAAAGCTGGGTTGAGAGGGCGA275970.21215559214526916No Hit
AAATGGATTTTTGGAGCAGGGA273910.2105719398648791No Hit
TGGCTCAGTTCAGCAGGAACAG272960.2098416147841167No Hit
TATTGCACTTGTCCCGGCCTGC271070.2083886522550209No Hit
AAATGGATTTTTGGAGCAGGGAGA269310.20703562894750313No Hit
TGTAAACATCCTTGACTGGAAGCT257070.19762596685431152No Hit
AGCTCGGTCTGAGGCCCCTCAGT251760.19354383403447104No Hit
AAATGGATTTTTGGAGCAGGGAG242840.18668646590773336No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT238860.18362678820096023No Hit
TGAGGTAGTAGGTTGTATAGC225260.17317160809741397No Hit
CTAGACTGAAGCTCCTTGAGG220920.16983517562319408No Hit
TGAGGGGCAGAGAGCGAGACTT220080.1691894144991515No Hit
TGTAAACATCCCCGACTGGAAG218770.1681823346509423No Hit
AAATGGATTTTTGGAGCAGGG218390.16789020461863732No Hit
AAACCGTTACCATTACTGAGA212110.16306237145317623No Hit
TTCAAGTAATCCAGGATAGGCG210810.1620629792373961No Hit
TGAGATGAAGCACTGTAGCTC205800.15821147539042793No Hit
TGAGGTAGTAGTTTGTACAGTT205030.15761952769338894No Hit
AGGAAACTCTGGTG199380.15327601537095978No Hit
TGAGGTAGTAGGTTGTGTGGC198840.15286088321978955No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT198470.15257644081991367No Hit
AAGCTGCCAGTTGAAGAACT196600.15113885355567605No Hit
TGAAAAGAACTTTGAAGAGAG193640.14886331435666894No Hit
AAACCGTTACCATTACTGAGTT189290.14551919425001994No Hit
AAGCTGCCAGTTGAAGAACTGC185850.14287464869441707No Hit
CGTGTATTTGACAAGCTGAGTTG180070.1384311971504099No Hit
ACCAATATTACTGTGCTGCTT178370.13712429963746664No Hit
CATTGCACTTGTCTCGGTCTG176410.1356175236813673No Hit
TACAGTACTGTGATAACTGAAG175000.1345335675088673No Hit
TGAGGTAGTAGATTGTATAG174900.13445669118457654No Hit
CAACGGAATCCCAAAAGCAGCT174600.1342260622117042No Hit
TGAGGTAGTAGGTTGTGTGGTC173450.13334198448236018No Hit
TGAGAACTGAATTCCATGGGTT170530.13109719581306936No Hit
TGTAAACATCCCCGACTGGAAGC169790.1305283110133176No Hit
TGAGGTAGTAGGTTGTAT168890.12983642409470056No Hit
TGAGGTAGTAGTTTGTGCTGT167590.1288370318789204No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT164410.12639236476647356No Hit
TGAGGTAGTAGGTTGTATAGTC160420.12332499942727139No Hit
GCGTCTGTAGTCCAAC159610.12270230120051606No Hit
GGCTGGTCCGATGGTAGTGGGTT154760.11897379947241316No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC153540.11803590831606563No Hit
TGGCTCAGTTCAGCAGGAACA152830.11749008641360108No Hit
TATTGCACTTGTCCCGGCCTG152380.11714414295429255No Hit
GTTGAAAAGAACTTTGAAGAGAG144230.11087872252459388No Hit
AAATGGATTTTTGGAGCAGGGAGAT141620.1088722504606045No Hit
TACAGTATAGATGATGTACT140630.10811117485012575No Hit
TCAGTGCATCACAGAACTTTGT135420.10410591835457605No Hit
GGCTGGTCCGATGGTAGTGGGT134800.10362928514397321No Hit
CGCGACCTCAGATCAGACGC133780.10284514663620724No Hit
TGAGGTAGTAGGTTGTGTGGTTT132220.10164587597727105No Hit
GGCTGGTCCGATGGTAGT131600.10116924276666821No Hit
AGAGGTAGTAGGTTGCATAGT130210.10010066185902634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)