FastQCFastQC Report
Thu 9 May 2024
EGAF00002167592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16473893
Total Bases355.1 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT4597532.7907975364414472No Hit
TCAGTGCACTACAGAACTTTGT1688331.0248518671330449No Hit
AAACCGTTACCATTACTGAGC868250.5270460358095078No Hit
TTCAAGTAATCCAGGATAGGCT747790.45392427885746256No Hit
TGAGGTAGTAGGTTGTATAGT538190.32669266456932794No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC525230.31882567162479447No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT425990.25858490157730174No Hit
TGAGGTAGTAGATTGTATAGT411410.24973453451470154No Hit
GGCCTTGAGGGCTGTTGTGGAGAAG397120.24106020355965646No Hit
TATTGCACTTGTCCCGGCCTGT382690.23230088965613652No Hit
TGAGGTAGTAGGTTGTATAGTT359160.21801768410174815No Hit
TCAGTGCACTACAGAACTTTGC337550.20489995898358696No Hit
TGAGGTAGTAGATTGTATAGTT324130.19675373635120735No Hit
CCCATAAAGTAGAAAGCACT323560.1964077343467024No Hit
TGAGGTAGTAGTTTGTGCTGTT319650.19403428200001058No Hit
AAGCTGCCAGTTGAAGAACTGT310950.1887531987733561No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT285840.17351089994332244No Hit
TGAGGTAGTAGTTTGTACAGT263800.1601321557691312No Hit
TAGCTTATCAGACTGATGTTGAC255140.15487535338489813No Hit
TCCTGTACTGAGCTGCCCCGAG252430.15323032631084832No Hit
TGAGGTAGTAGGTTGTGTGGT240640.14607354800714073No Hit
AGCGGTGTCGGGATCTTCCCCTCGGAC238460.14475024209517445No Hit
CGCGACCTCAGATCAGA232790.14130843268194104No Hit
TAGCTTATCAGACTGATGTTGA222260.13491650091450758No Hit
CGCGACCTCAGATCAGACGT213910.12984787505904039No Hit
TGAGGTAGTAGGTTGTGTGGTT205000.12443931740967361No Hit
TCCTGTACTGAGCTGCCCCGA200340.12161059926758054No Hit
CGGACACGCTACCTGCGGGACTGTGC199150.12088824420554388No Hit
TGAGGGGCAGAGAGCGAGACTTT196680.11938890218602247No Hit
AAACCGTTACCATTACTGAG178470.10833504867368021No Hit
TTCACAGTGGCTAAGTTCCG178460.10832897846307488No Hit
TTCAAGTAATCCAGGATAGGCC178220.10818329340854649No Hit
GGGCCTTGAGGGCTGTTGTGGAGAAG166880.10129967458207965No Hit
AACGAGACTCTAGCCTGCTAACT166740.10121469163360476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)