FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13524170
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8024415.933384451689086No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5388283.984185351115817No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3292652.434641090728673No Hit
TCCTGTACTGAGCTGCCCCGAG2668461.9731044492933763No Hit
CGCGACCTCAGATCAGA2429481.79639859599517No Hit
TTCAAGTAATCCAGGATAGGCT2206281.6313607415464313No Hit
TCCTGTACTGAGCTGCCCCGA2200201.6268650867299066No Hit
TAGCTTATCAGACTGATGTTGAC1878281.388831994865489No Hit
TGAGGTAGTAGGTTGTATAGT1650701.2205554943482668No Hit
TGAGGTAGTAGGTTGTATAGTT1534121.1343542709090464No Hit
TATTGCACTTGTCCCGGCCTGT1423991.0529222865432777No Hit
TCAGTGCACTACAGAACTTTGT1360961.0063168386673638No Hit
TGAGGTAGTAGATTGTATAGTT1234880.9130911545773234No Hit
TCGTACCGTGAGTAATAATGCG1141050.8437116658545404No Hit
TCCTGTACTGAGCTGCCCCGAGA1111420.8218027427930883No Hit
TGAGGTAGTAGATTGTATAGT1069020.7904514657831128No Hit
TGAGGGGCAGAGAGCGAGACTTT995370.7359934103164927No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT953760.7052262726659011No Hit
TGAGGTAGTAGGTTGTGTGGTT933890.6905340586520282No Hit
TGAGGTAGTAGGTTGTGTGGT890020.6580958387834521No Hit
TGAGGTAGTAGTTTGTGCTGTT849830.6283786731459305No Hit
AAACCGTTACCATTACTGAGC834060.6167180684655694No Hit
AAGCTGCCAGTTGAAGAACTGT701710.518856240345988No Hit
TCCTGTACTGAGCTGCCCCG669130.4947660373982285No Hit
TAGCTTATCAGACTGATGTTGA595740.44050023032836766No Hit
TGAGGTAGTAGTTTGTACAGT572170.423072173745228No Hit
TGAGGGGCAGAGAGCGAGACT549520.40632438071985194No Hit
TGAGGTAGTAGGTTGTATAG512490.3789437725198663No Hit
TGGCTCAGTTCAGCAGGAAC487360.3603622255561709No Hit
AAACCGTTACCATTACTGAG482240.35657641097383425No Hit
CATTGCACTTGTCTCGGTCTGA479710.3547056861899843No Hit
TCCTGTACTGAGCTGCCCCGAGT470670.3480213573180461No Hit
TGGAGTGTGACAATGGTGTTT446770.3303492931544043No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC421060.31133888438255364No Hit
TGTAAACATCCCCGACTGGAAGCT409330.3026655240210675No Hit
AAAAGCTGGGTTGAGAGGGCGA396480.29316401671969516No Hit
TAGCTTATCAGACTGATGTTG391510.28948911467395044No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC374570.2769633922081725No Hit
CGCGACCTCAGATC372300.27528491582108183No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT362870.2683122143540047No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT362810.268267849339368No Hit
CTAGACTGAAGCTCCTTGAGG360770.26675943884171816No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC359630.26591650356361984No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA357780.2645485822789864No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAC351600.2599789857714004No Hit
CGCGACCTCAGATCAGACGT343630.25408583299381776No Hit
CCCATAAAGTAGAAAGCACT343020.25363478867834405No Hit
TGAGGTAGTAGGTTGTGTGG335350.24796346097394517No Hit
AAACCGTTACCATTACTG311170.2300843600753318No Hit
TATTGCACTTGTCCCGGCCTG309890.22913790642974763No Hit
TTCACAGTGGCTAAGTTCCG303870.2246866166278596No Hit
TTCAAGTAATTCAGGATAGGT297330.219850830032453No Hit
TGAGGTAGTAGTTTGTACAGTT297020.21962161079016307No Hit
TGTAAACATCCCCGACTGGAAGC293230.21681922069894125No Hit
TGGCTCAGTTCAGCAGGAACAG285170.21085952039940342No Hit
TGAGGTAGTAGATTGTATAG282950.20921801485784342No Hit
TTCAAGTAATCCAGGATAGGCC281990.20850817462365528No Hit
GGTGGGCGTAGCTC259030.19153116235598933No Hit
TATTGCACTTGTCCCGGCCTGTA239430.1770385909079818No Hit
CAACGGAATCCCAAAAGCAGCTG239090.1767871891583735No Hit
AGCTCGGTCTGAGGCCCCTCAGT238710.17650621073234068No Hit
GCGGACGTGGTGGAATTGGTAGACACAC234860.17365945562648205No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG234020.17303834542156746No Hit
TGTAAACATCCCCGACTGGAAG233330.17252814775324476No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT232920.17222498681989357No Hit
TACAGTACTGTGATAACTGAAG230010.1700732836100108No Hit
CATTGCACTTGTCTCGGTCTG226850.1677367261724749No Hit
CAACGGAATCCCAAAAGCAGCT201710.1491477850396734No Hit
TGAGGGGCAGAGAGCGAGACTT200160.14800168882822384No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA197710.1461901173972229No Hit
TGAGGTAGTAGGTTGTAT194590.1438831366361115No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT187290.1384853931886393No Hit
CGCGACCTCAGATCAGAC183120.13540202467138465No Hit
AGAGGTAGTAGGTTGCATAGTT182270.13477352029736392No Hit
GTGAAATGTTTAGGAC181120.13392319085015938No Hit
TGAGAACTGAATTCCATGGGTT180010.13310243807937938No Hit
TCGTACCGTGAGTAATAATGC178020.13163099842726023No Hit
TGAGGTAGTAGGTTGTATAGC174710.12918352845313244No Hit
TGAGGTAGTAGATTGTAT171080.12649944506760857No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA166800.12333474069018653No Hit
CTAGACTGAAGCTCCTTGAGGA166440.12306855060236599No Hit
AGAGGTAGTAGGTTGCATAGT163320.12076156984125459No Hit
AGCAGCATTGTACAGGGCTATG162410.12008870045259708No Hit
GTTTCCGTAGTGTAGTGGTTATC156360.11561522814339069No Hit
TGTAAACATCCTTGACTGGAAGCT154280.11407724096931642No Hit
TATTGCACTTGTCCCGGCCTGC152890.11304945146356486No Hit
AACATTCAACGCTGTCGGTGA148770.11000305379184083No Hit
GGCTGGTCCGATGGTAGTGGGTTATC148250.10961855699832226No Hit
TCAGTGCACTACAGAACTTTGC147680.10919708935927307No Hit
AAGCTGCCAGTTGAAGAACT147190.10883477507307288No Hit
TAGCTTATCAGACTGATGTTGACA145070.1072672112225741No Hit
TGAGGTAGTAGTTTGTGCTGT142890.10565528235743857No Hit
TAGCAGCACGTAAATATTGGC139830.10339266661096394No Hit
AAAAGCTGGGTTGAGAGGGCG138780.10261627885482068No Hit
TACAGTATAGATGATGTACT137290.10151454765800784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)