FastQCFastQC Report
Wed 8 May 2024
EGAF00002167595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13449588
Total Bases317.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6514964.843984812025469No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4926423.6628780004264816No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT4220043.13767232126367No Hit
CGCGACCTCAGATCAGA3069422.2821665615333346No Hit
TTCAAGTAATCCAGGATAGGCT2941592.1871227579610615No Hit
TAGCTTATCAGACTGATGTTGAC2517021.8714476607015769No Hit
TCCTGTACTGAGCTGCCCCGAG2085301.550456415467894No Hit
TCCTGTACTGAGCTGCCCCGA1942791.4444977794115328No Hit
TGAGGTAGTAGGTTGTATAGTT1689211.2559566880412991No Hit
TATTGCACTTGTCCCGGCCTGT1591911.1836124645602526No Hit
TGAGGTAGTAGATTGTATAGTT1427671.0614971997655245No Hit
TCGTACCGTGAGTAATAATGCG1351451.0048263188433726No Hit
TCAGTGCACTACAGAACTTTGT1349371.0032798030690606No Hit
TGAGGTAGTAGGTTGTATAGT1208890.8988305069270524No Hit
TGGAGTGTGACAATGGTGTTT1160780.863059894474091No Hit
TCCTGTACTGAGCTGCCCCGAGA925760.6883184823207967No Hit
TGAGGTAGTAGTTTGTGCTGTT883010.656533122055486No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT882330.6560275303600378No Hit
TGAGGGGCAGAGAGCGAGACTTT841960.6260117410287958No Hit
TGAGGTAGTAGATTGTATAGT794920.5910366919789662No Hit
AAACCGTTACCATTACTGAGC776300.5771924017300754No Hit
AAGCTGCCAGTTGAAGAACTGT751380.5586639531262965No Hit
TGAGGTAGTAGGTTGTGTGGTT699190.5198597905006458No Hit
TAGCTTATCAGACTGATGTTGA672690.5001565847221491No Hit
TGAGGTAGTAGGTTGTGTGGT604880.44973868344517315No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC555830.41326916482497456No Hit
TGTAAACATCCCCGACTGGAAGCT554480.41226541660606997No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT550030.4089567650696809No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC543910.40440643981064694No Hit
TCCTGTACTGAGCTGCCCCG542370.4032614233238966No Hit
CGCGACCTCAGATCAGACGT540000.4014992875618197No Hit
TCCTGTACTGAGCTGCCCCGAGT496040.36881427148549084No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT473780.3522635786315536No Hit
TGAGGTAGTAGTTTGTACAGT473090.3517505517641135No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC460460.3423599295383621No Hit
TTCAAGTAATCCAGGATAGGCC448710.3336236024478966No Hit
AAAAGCTGGGTTGAGAGGGCGA442840.3292591564886597No Hit
CTAGACTGAAGCTCCTTGAGG437840.3255415704927169No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA433890.32260467755592215No Hit
TGGCTCAGTTCAGCAGGAAC418390.31108016096849955No Hit
TGAGGGGCAGAGAGCGAGACT410310.30507254199905603No Hit
TAGCTTATCAGACTGATGTTG399840.2972879169235519No Hit
CGCGACCTCAGATC379050.2818301943524218No Hit
CATTGCACTTGTCTCGGTCTGA373550.27774084975688473No Hit
TGAGGTAGTAGGTTGTATAG364840.2712648149519524No Hit
TGAGGTAGTAGTTTGTACAGTT361820.269019393010403No Hit
TGTAAACATCCCCGACTGGAAGC356340.26494491875884973No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT349760.260052575588189No Hit
AAACCGTTACCATTACTGAG318340.2366912651896846No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG306420.22782854017535706No Hit
AGCTCGGTCTGAGGCCCCTCAGT296480.2204379792154228No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT292500.21747878076265234No Hit
TGGCTCAGTTCAGCAGGAACAG289080.2149359519414275No Hit
TATTGCACTTGTCCCGGCCTG285160.21202136452060835No Hit
CCCATAAAGTAGAAAGCACT271330.20173852165583064No Hit
TTCAAGTAATTCAGGATAGGT268100.1993369611024516No Hit
CAACGGAATCCCAAAAGCAGCTG267030.19854139769931986No Hit
CGCGACCTCAGATCAGAC248000.18439226539876166No Hit
TTCACAGTGGCTAAGTTCCG244600.18186430692152056No Hit
TGAGAACTGAATTCCATGGGTT240380.17872666434094486No Hit
TATTGCACTTGTCCCGGCCTGTA235830.17534366108463695No Hit
AGAGGTAGTAGGTTGCATAGTT231220.1719160467963777No Hit
GTTTCCGTAGTGTAGTGGTTATC228870.1701687813782846No Hit
TGAGGTAGTAGGTTGTGTGG221100.16439165274058953No Hit
AAACCGTTACCATTACTG217880.1619975273592024No Hit
TATTGCACTTGTCCCGGCCTGC215710.16038409503696321No Hit
TGGAGTGTGACAATGGTGTTTG214040.15914242131431833No Hit
CAACGGAATCCCAAAAGCAGCT207740.15445826295943044No Hit
TCGTACCGTGAGTAATAATGC207460.15425007814365763No Hit
CTAGACTGAAGCTCCTTGAGGA203990.15167007346247333No Hit
TGAGGTAGTAGATTGTATAG199190.14810119090636828No Hit
TGTAAACATCCCCGACTGGAAG191480.14236867330062453No Hit
TAGCTTATCAGACTGATGTTGACA190860.1419076926371276No Hit
TACCCTGTAGAACCGAATTTGT190700.14178872988525745No Hit
TACAGTACTGTGATAACTGAAG188170.1399076313713104No Hit
TAGCTTATCAGACTGATGTTGACT186780.13887414246443833No Hit
TGAGGTAGTAGGTTGTATAGTC181290.13479223304089313No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC175880.13076980499328306No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC173460.12897049337124677No Hit
TCAGTGCACTACAGAACTTTGC171700.1276619031006749No Hit
AACCCGTAGATCCGATCTTGT169930.12634587765811114No Hit
TGAGGGGCAGAGAGCGAGACTT169050.12569158252282525No Hit
TGTAAACATCCTTGACTGGAAGCT165130.1227769951020061No Hit
TGAGGTAGTAGATTGTATAGTTT164880.12259111580220897No Hit
CATTGCACTTGTCTCGGTCTG163280.12140148828350726No Hit
TCGTACCGTGAGTAATAATGCA160380.11924528840586046No Hit
TGAGGTAGTAGGTTGTATAGC151840.1128956515247902No Hit
TGAGGTAGTAGATTGTATAGTC151700.11279155911690379No Hit
CGCGACCTCAGATCAGACG151380.11255363361316345No Hit
TGAGGTAGTAGGTTGTAT151320.11250902258121216No Hit
TTCAAGTAATTCAGGATAGGTT150280.11173576469405606No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC148020.11005541582388993No Hit
AAGCTGCCAGTTGAAGAACT147310.10952751861246605No Hit
AGAGGTAGTAGGTTGCATAGT142500.1059512008843691No Hit
TGAGGTAGTAGATTGTAT141210.10499206369741584No Hit
GGCTGGTCCGATGGTAGTGGGTTATC139740.10389909341460869No Hit
TGAGGTAGTAGTTTGTGCTGT139440.10367603825485211No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA137150.10197338386871034No Hit
AAAAGCTGGGTTGAGAGGGCG136590.10155701423716473No Hit
TGGAGAGAAAGGCAGTTCCTGA136240.10129678321744873No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA135900.10104398736972464No Hit
TCACAGTGAACCGGTCTCTTT135620.10083580255395184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)