FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12862132
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC9896997.694673013774077No Hit
AAACCGTTACCATTACTGAGT9575107.444411237577099No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT6970465.4193659340457705No Hit
TAGCTTATCAGACTGATGTTGAC2304321.7915536864339443No Hit
TTCAAGTAATCCAGGATAGGCT2161281.680343507592676No Hit
TCAGTGCACTACAGAACTTTGT1564181.216112538729971No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1462651.1371753920734136No Hit
TCCTGTACTGAGCTGCCCCGAG1432481.1137189386642898No Hit
TAGCTTATCAGACTGATGTTGA1404231.0917552393335723No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1258570.9785080731561455No Hit
AAACCGTTACCATTACTGAG1092690.8495403405905023No Hit
AAACCGTTACCATTACTGAGC1092480.8493770706131767No Hit
AAGCTGCCAGTTGAAGAACTGT866070.6733487107736105No Hit
TAGCTTATCAGACTGATGTTG856770.6661181832063301No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT848540.659719555047328No Hit
CGCGACCTCAGATCAGA841690.6543938438821806No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC831550.6465102364055975No Hit
TCGTACCGTGAGTAATAATGCG829120.6446209695251145No Hit
TCCTGTACTGAGCTGCCCCGA794220.6174870542457502No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG790940.6149369326951395No Hit
TGAGGTAGTAGTTTGTGCTGTT765180.5949091488098551No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT762710.592988782886072No Hit
TTCACAGTGGCTAAGTTCCG749600.5827960714444542No Hit
TATTGCACTTGTCCCGGCCTGT699060.5435024302347387No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT689150.5357976422571312No Hit
CGCGACCTCAGATC639290.49703268478351803No Hit
CGCGACCTCAGATCAGACGT629750.48961556295643677No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA577690.44914015810131636No Hit
TGGCTCAGTTCAGCAGGAAC520830.4049328680501802No Hit
TGAGGTAGTAGTTTGTACAGT515150.40051680390156164No Hit
CATTGCACTTGTCTCGGTCTGA507290.3944058418930858No Hit
TACAGTACTGTGATAACTGAAG500170.38887021218566253No Hit
CCCATAAAGTAGAAAGCACT495610.3853249212494476No Hit
TGAGGTAGTAGATTGTATAGTT466410.362622619640352No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT405810.3155075690406536No Hit
TGAGGTAGTAGGTTGTATAGTT380200.2955964065677448No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC378090.2939559320336628No Hit
TCCTGTACTGAGCTGCCCCGAGA367900.2860334507529545No Hit
AAAAGCTGGGTTGAGAGGGCGA361810.28129862141050954No Hit
TGAGGGGCAGAGAGCGAGACTTT344330.2677083394883523No Hit
AAACCGTTACCATTACTGAGTT341900.26581907260786936No Hit
TGAAAAGAACTTTGAAGAGAG335820.2610920180262495No Hit
CGCGACCTCAGATCAGACG318810.24786714986286879No Hit
TGAGGTAGTAGGTTGTATAGT317950.24719852043191595No Hit
TGAGGTAGTAGATTGTATAGT316140.24579128872258502No Hit
GTTTCCGTAGTGTAGTGGTTATC314910.24483499314110602No Hit
TACAGTATAGATGATGTACT312910.24328004097609943No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA312770.243171194324549No Hit
TGAGGTAGTAGGTTGTGTGGTT311160.2419194578317187No Hit
TGTAAACATCCTTGACTGGAAGCT310410.24133635076984128No Hit
TTCAAGTAATCCAGGATAGGCC310250.24121195459664074No Hit
TCCTGTACTGAGCTGCCCCG302700.23534201017374104No Hit
TGAGGTAGTAGGTTGTGTGGT299990.23323504999015715No Hit
TGTAAACATCCCCGACTGGAAGCT299700.2330095819262312No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT289380.22498602875479745No Hit
TGAGAACTGAATTCCATGGGTT277710.2159128828719842No Hit
TGAGGTAGTAGGTTGTATAG272700.21201772769864283No Hit
TTCAAGTAATTCAGGATAGGT265200.20618665707986825No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC263760.20506709152106353No Hit
TGTAAACATCCCCGACTGGAAG262750.20428184067773522No Hit
CTAGACTGAAGCTCCTTGAGG259900.2020660338426009No Hit
AAACCGTTACCATTACTG259610.20184056577867496No Hit
TGAGGTAGTAGTTTGTACAGTT259050.2014051791724731No Hit
TGAGATGAAGCACTGTAGCTC255980.19901832759918808No Hit
TGAGGTAGTAGTTTGTGCTGT255660.198769535252787No Hit
CAACGGAATCCCAAAAGCAGCTG248890.19350602217423984No Hit
GCATTGGTGGTTCAGTGGTAGAATT247710.192588600396886No Hit
TACAGTACTGTGATAACTGAA235790.18332108549344697No Hit
TACAGTACTGTGATAACTGA233420.18147846717791422No Hit
CATTGCACTTGTCTCGGTCTG233240.18133852148306362No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC226670.1762305036210171No Hit
TGAGGGGCAGAGAGCGAGACT207350.16120966570705386No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG202930.1577732214223894No Hit
TGAGGTAGTAGATTGTATAG202230.1572289881646371No Hit
TGGCTCAGTTCAGCAGGAACAG201050.15631156638728322No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT200480.15586840502025634No Hit
TGGAGTGTGACAATGGTGTTT197550.15359040009852176No Hit
TCAGTGCACTACAGAACTTTGC194980.15159228656648835No Hit
TCCTGTACTGAGCTGCCCCGAGT189620.1474250147642708No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG188810.14679525913744315No Hit
TGTAAACATCCCCGACTGGAAGC186140.1447193979971594No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC183620.14276015826925115No Hit
TGGAGAGAAAGGCAGTTCCTG181150.1408397923454681No Hit
TCAGTGCATCACAGAACTTTGT179590.13962692965676296No Hit
TGGAGAGAAAGGCAGTTCCTGA176060.13688243908552641No Hit
AGGAAACTCTGGTG174520.13568512591847137No Hit
TGAGGTAGTAGTTTGTGCTG172290.13395135425448906No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC162810.12658088099235804No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA155590.12096750367668441No Hit
CGCGACCTCAGATCAGAC155260.12071093656945832No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC151120.11749218558789476No Hit
TAGCAGCACGTAAATATTGGCG150910.11732891561056907No Hit
AACATTCAACGCTGTCGGTGA150220.11679245711364182No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC149720.11640371907239017No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC147790.11490319023315886No Hit
TGAGGTAGTAGGTTGTGTGG146890.11420346175890592No Hit
TAAAGTGCTTATAGTGCAGGTAG143470.11154449355674473No Hit
TTCACAGTGGCTAAGTTCCGA143430.1115133945134446No Hit
TGAGATGAAGCACTGTAGCT140100.10892439915870869No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG138990.10806140070713005No Hit
TAGCAGCACGTAAATATTGGC138280.10750939268855271No Hit
AAAAGCTGGGTTGAGAGGGCG135280.10517696444104291No Hit
GTACAGTACTGTGATAACTGA131850.10251022147805668No Hit
TCGTACCGTGAGTAATAATGC130970.1018260425254538No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCC130750.10165499778730307No Hit
TAGTGGGTTATCAGAACT129580.10074535077077423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)