FastQCFastQC Report
Thu 9 May 2024
EGAF00002167598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11749731
Total Bases274.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT117608910.00949723870274No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3695133.1448634866619503No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3024342.573965310354765No Hit
TCAGTGCACTACAGAACTTTGT2830192.4087274849100804No Hit
TTCAAGTAATCCAGGATAGGCT2790082.3745905331790147No Hit
AAACCGTTACCATTACTGAGC2504962.131929658644951No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1924721.6380970764351965No Hit
AAGCTGCCAGTTGAAGAACTGT1292291.099846456059292No Hit
TAGCTTATCAGACTGATGTTGAC1199171.020593577844463No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1009080.8588111506552788No Hit
TATTGCACTTGTCCCGGCCTGT989800.8424022643582224No Hit
TGAGGTAGTAGGTTGTATAGTT979580.8337041928874797No Hit
TAGCTTATCAGACTGATGTTGA932810.793899026284091No Hit
TGAGGTAGTAGATTGTATAGTT860560.7324082568358373No Hit
TGAGGGGCAGAGAGCGAGACTTT773940.6586874201630659No Hit
TTCAAGTAATCCAGGATAGGCC711110.6052138555342246No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC696680.592932723310857No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT690410.5875964309310571No Hit
CGCGACCTCAGATCAGA664620.5656469922588014No Hit
TCCTGTACTGAGCTGCCCCGAG656150.5584383165878436No Hit
TGAGGTAGTAGGTTGTATAGT651030.5540807700193306No Hit
TCGTACCGTGAGTAATAATGCG636150.54141665030459No Hit
TCAGTGCACTACAGAACTTTGC623240.5304291647187497No Hit
TCCTGTACTGAGCTGCCCCGA595520.5068371352501602No Hit
CCCATAAAGTAGAAAGCACT549010.4672532503084538No Hit
AAACCGTTACCATTACTGAG546610.46521065035446346No Hit
TTCACAGTGGCTAAGTTCCG545280.46407870954662706No Hit
TGAGGTAGTAGTTTGTGCTGTT540740.4602147913003285No Hit
TGGAGTGTGACAATGGTGTTT531230.4521209889826414No Hit
TGAGGTAGTAGATTGTATAGT461520.39279197115236086No Hit
TAGCTTATCAGACTGATGTTG461060.392400472827846No Hit
TGGCTCAGTTCAGCAGGAAC454980.3872258862777369No Hit
TGAGGTAGTAGGTTGTGTGGTT453800.3862216079670249No Hit
TGAGGTAGTAGGTTGTGTGGT409890.3488505396421416No Hit
TGAGGGGCAGAGAGCGAGACT384070.3268755684704612No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC374500.3187307011539243No Hit
TGTAAACATCCCCGACTGGAAGCT359970.3063644605991405No Hit
CGCGACCTCAGATCAGACGT359590.3060410489397587No Hit
TGAGGTAGTAGTTTGTACAGT354550.3017515890363788No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT346930.2952663341824592No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT336710.28656826271171654No Hit
TGAGAACTGAATTCCATGGGTT327690.27889149121796913No Hit
TTCAAGTAATCCAGGATAGGCA318060.27069555890258257No Hit
CATTGCACTTGTCTCGGTCTGA313970.2672146281476572No Hit
AAGCTGCCAGTTGAAGAACTGC306120.2605336241314801No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA305550.26004850664240736No Hit
AAAAGCTGGGTTGAGAGGGCGA301830.2568824767137222No Hit
TGTAAACATCCTTGACTGGAAGCT282520.24044805791724083No Hit
TATTGCACTTGTCCCGGCCTGC266470.2267881707249298No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC260920.22206465833132688No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG248210.21124738940831922No Hit
TGAGATGAAGCACTGTAGCT241150.2052387412103307No Hit
CTAGACTGAAGCTCCTTGAGG236320.20112800880292495No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT234370.19946839634030772No Hit
TCAGTGCATCACAGAACTTTGT226590.19284696815612204No Hit
TTCAAGTAATTCAGGATAGGT226410.19269377315957276No Hit
TGAGGTAGTAGTTTGTGCTGT222700.1895362540640292No Hit
TGAGGTAGTAGGTTGTATAGTC220810.18792770660026173No Hit
TAGCTTATCAGACTGATGTTGAT220510.18767238160601296No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT219020.18640426746791053No Hit
TGAGGTAGTAGGTTGTATAG212770.18108499675439377No Hit
CGCGACCTCAGATC212100.18051477093390478No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC203040.1728039561075909No Hit
AGGAAACTCTGGTG202650.17247203361506744No Hit
AAACCGTTACCATTACTGAGA202550.17238692528365118No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT201110.17116136531125692No Hit
TCGTACCGTGAGTAATAATGCA198920.16929749285324064No Hit
TGAGGTAGTAGTTTGTACAGTT195440.1663357229199545No Hit
TCCTGTACTGAGCTGCCCCGAGA194950.16591869209601479No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA194480.16551868293835834No Hit
TGGCTCAGTTCAGCAGGAACAG193360.16456546962649612No Hit
TGAGGTAGTAGATTGTATAGTC189910.16162923219263486No Hit
TGAGATGAAGCACTGTAGCTC188540.160463248052232No Hit
TCCTGTACTGAGCTGCCCCGAGT177800.1513226132581248No Hit
AGCTCGGTCTGAGGCCCCTCAGT174710.14869276581736213No Hit
TACAGTACTGTGATAACTGAAG171860.14626717837199849No Hit
TGAGGTAGTAGGTTGTGTGG170160.1448203367379219No Hit
TCCTGTACTGAGCTGCCCCG167830.14283731261592286No Hit
AAACCGTTACCATTACTGAGTT165650.1409819509910482No Hit
GCATTGGTGGTTCAGTGGTAGAATT160050.1362158844317372No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT159800.13600311360319653No Hit
TGTAAACATCCCCGACTGGAAG157200.13379029698637354No Hit
TTCACAGTGGCTAAGTTCCA152630.1299008462406501No Hit
TAGTGGGTTATCAGAACT151830.12921997958931997No Hit
TGTAAACATCCCCGACTGGAAGC151280.1287518837665305No Hit
AAACCGTTACCATTACTG150030.12768802962382714No Hit
AACCCGTAGATCCGATCTTGT142550.12132192643389027No Hit
TGAGGTAGTAGGTTGTATAGC141950.12081127644539268No Hit
AAGCTGCCAGTTGAAGAACT141920.12078574394596778No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT141520.12044531062030271No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA137320.11687076070081945No Hit
TGAGGGGCAGAGAGCGAGACTT136430.11611329655121466No Hit
ACCAATATTACTGTGCTGCTT136070.1158069065581161No Hit
TAGCTTATCAGACTGATGTTGACT134330.11432602159147302No Hit
TACAGTATAGATGATGTACT132720.1129557774556711No Hit
CAACGGAATCCCAAAAGCAGCTG132030.11236852996889886No Hit
TGAGGGGCAGAGAGCGAGACTTC129610.11030890834862518No Hit
AACATTCAACGCTGTCGGTGA128720.1095514441990204No Hit
AAACCGTTACCATTACTGAGG127450.10847056839003379No Hit
TGGAGAGAAAGGCAGTTCCTGA123850.10540666845904813No Hit
TTCAAGTAATTCAGGATAGGTT122770.10448749847975242No Hit
CCCATAAAGTAGAAAGCACC122640.10437685764891128No Hit
AGAGGTAGTAGGTTGCATAGTT121910.10375556682957253No Hit
GGCTGGTCCGATGGTAGTGGGTT121630.10351726350160696No Hit
GGCTGGTCCGATGGTAGTGGGT120780.1027938426845687No Hit
TACCCTGTAGAACCGAATTTGT119540.10173849937500698No Hit
AGTTGGTCCGAGTGTTGT118970.10125338188593425No Hit
TGAGGTAGTAGTTTGTGCTGTC118530.10087890522770267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)