FastQCFastQC Report
Wed 8 May 2024
EGAF00002167600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12424427
Total Bases284.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9496347.6432820604121225No Hit
TCAGTGCACTACAGAACTTTGT6656275.357406019609597No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2123941.70948728661692No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1693471.3630165801610006No Hit
AAACCGTTACCATTACTGAGC1536811.2369262582491733No Hit
CGCGACCTCAGATCAGA1518971.2225674471748276No Hit
TTCAAGTAATCCAGGATAGGCT1246851.0035472863255586No Hit
TCAGTGCACTACAGAACTTTGC1173420.9444459692185402No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1060400.8534800035446303No Hit
TCCTGTACTGAGCTGCCCCGAG1028350.8276840453084879No Hit
TATTGCACTTGTCCCGGCCTGT904760.7282106450462464No Hit
AAGCTGCCAGTTGAAGAACTGT851130.685045676553132No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC836180.6730129284835429No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT834990.6720551378345255No Hit
TGAGGGGCAGAGAGCGAGACTTT818980.6591692317078285No Hit
CGCGACCTCAGATCAGACGT768490.6185315427423735No Hit
TGAGGTAGTAGGTTGTATAGT761630.6130101613539201No Hit
CATTGCACTTGTCTCGGTCTGA760050.6117384729291742No Hit
TGAGGTAGTAGGTTGTATAGTT755230.6078590183676076No Hit
TGAGGTAGTAGTTTGTGCTGTT708540.5702798205502757No Hit
TCCTGTACTGAGCTGCCCCGA704120.5667223124253538No Hit
TGAGGTAGTAGGTTGTGTGGT633300.509721695817441No Hit
TGAGGGGCAGAGAGCGAGACT613440.49373705523804035No Hit
TAGCTTATCAGACTGATGTTGAC610460.49133855428503864No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT550160.4428051289608768No Hit
TAGCTTATCAGACTGATGTTGA532950.42895338352424617No Hit
TGAGGTAGTAGATTGTATAGTT511350.41156827594544204No Hit
TGAGGTAGTAGGTTGTGTGGTT502230.4042278971899469No Hit
TGAGGTAGTAGATTGTATAGT488500.3931770857521237No Hit
TGGCTCAGTTCAGCAGGAAC462790.37248397853679693No Hit
TGAGGTAGTAGTTTGTACAGT455840.3668901592000983No Hit
TCGTACCGTGAGTAATAATGCG453470.36498262656297953No Hit
AAACCGTTACCATTACTGAG428080.3445470764969684No Hit
TGGAGTGTGACAATGGTGTTT426240.3430661228884036No Hit
CCCATAAAGTAGAAAGCACT416910.3355567222536701No Hit
AAAAGCTGGGTTGAGAGGGCGA411450.3311621533934724No Hit
AAACCGTTACCATTACTGAGTT410790.3306309417730089No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC404740.3257615019187605No Hit
TTCACAGTGGCTAAGTTCCG381540.30708860859337817No Hit
TGAGGTAGTAGTTTGTACAGTT364780.29359905289797267No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT335660.2701613523102514No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC329600.2652838637950869No Hit
TGGCTCAGTTCAGCAGGAACAG312750.2517218701514363No Hit
TGAGAACTGAATTCCATGGGTT273790.22036428722225984No Hit
TGAGATGAAGCACTGTAGCT265890.2140058450985305No Hit
TCCTGTACTGAGCTGCCCCGAGA265890.2140058450985305No Hit
TGAGATGAAGCACTGTAGCTC265540.21372414196646652No Hit
CTAGACTGAAGCTCCTTGAGG264370.2127824486392813No Hit
TTCAAGTAATCCAGGATAGGCC259450.2088225074685537No Hit
CATTGCACTTGTCTCGGTCTG253420.2039691649361375No Hit
TCCTGTACTGAGCTGCCCCG244830.19705536520919636No Hit
CGCGACCTCAGATC234790.18897450964941884No Hit
CGTGTATTTGACAAGCTGAGTTG229640.1848294492776206No Hit
TGAGGGGCAGAGAGCGAGACTT223610.17997610674520442No Hit
TGAGGTAGTAGGTTGTATAG221130.1779800388380084No Hit
TAGCTTATCAGACTGATGTTG217510.17506642358637547No Hit
TGAGGTAGTAGGTTGTGTGG216960.17462374723598925No Hit
TGAGGTAGTAGTTTGTGCTGT212710.17120306634664118No Hit
TGAAAAGAACTTTGAAGAGAG208700.16797555331927982No Hit
CCGAAGAGGGGGACG204140.16430536394153228No Hit
GCATTGGTGGTTCAGTGGTAGAATT199790.1608041964430231No Hit
TGTCAGTTTGTCAAATACCCCAA196180.15789862985230627No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT195290.1571822990307722No Hit
TATTGCACTTGTCCCGGCCTGC193820.15599914587610358No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC191540.15416405118722978No Hit
TCCTGTACTGAGCTGCCCCGAGT190700.1534879636702763No Hit
TGTAAACATCCTTGACTGGAAGCT190330.15319016321638013No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC174950.1408113227273982No Hit
AAACCGTTACCATTACTG174900.14077107942281764No Hit
AAATGGATTTTTGGAGCAGGGAGA173510.13965231555547794No Hit
TGTCAGTTTGTCAAATACCCCA173100.1393223204579173No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC171860.13832428650431927No Hit
TGTAAACATCCCCGACTGGAAG166220.1337848417476315No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG163280.13141853543829426No Hit
TCAGTGCATCACAGAACTTTGT158310.12741835096298607No Hit
TGGCTCAGTTCAGCAGGAACA157450.12672616612420035No Hit
AAGCTGCCAGTTGAAGAACTGC154240.12414254597012803No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG153860.12383669685531574No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA152880.12304792808553666No Hit
CGCGACCTCAGATCAGACGC150090.12080235168994112No Hit
AAGGACGGAGTGGAACGTGCGGAGAGT148700.11968358782260138No Hit
AGCTCGGTCTGAGGCCCCTCAGT148110.11920871682855072No Hit
TGTAAACATCCCCGACTGGAAGCT143810.1157477926346221No Hit
AGGAAACTCTGGTG140070.11273759345199581No Hit
TGTCAGTTTGTCAAATACCCCAT137730.11085420679762537No Hit
ACGGCCCTGGCGGAGC135600.10913984202249327No Hit
TGAGGTAGTAGGTTGTATAGTC134110.10794059154599243No Hit
AAAAGCTGGGTTGAGAGGGCG133230.10723230938537447No Hit
GGCTGGTCCGATGGTAGTGGGTTATC128970.1038035798351103No Hit
CGCGACCTCAGATCAGACG128590.10349773072029801No Hit
AAAAGCTGGGTTGAGAGGGCGT127570.10267676730685447No Hit
TGAGGTAGTAGGTTGTATAGC125650.10113142441096076No Hit
TGAGGTAGTAGTTTGTGCTGTC125480.10099459717538685No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA125120.10070484538240677No Hit
GGGTGCTGTAGGCTT124740.10039899626759446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)