FastQCFastQC Report
Fri 9 Sep 2022
EGAF00002167601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8839541
Sequences flagged as poor quality0
Sequence length14-50
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7959829.004788823311074No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5529286.255166416446284No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2984073.375820079345749No Hit
TCCTGTACTGAGCTGCCCCGA2399312.714292518129618No Hit
TCCTGTACTGAGCTGCCCCGAG1618171.8306041003712752No Hit
AAACCGTTACCATTACTGAGC1030701.1660107691112016No Hit
CGCGACCTCAGATCAGA981121.1099218839530243No Hit
TTCAAGTAATCCAGGATAGGCT913771.0337301450380738No Hit
TAGCTTATCAGACTGATGTTGAC787310.890668418190492No Hit
TGAGGGGCAGAGAGCGAGACTTT730240.8261062423942601No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT716360.8104040696230721No Hit
TATTGCACTTGTCCCGGCCTGT661410.748240208399961No Hit
TCCTGTACTGAGCTGCCCCG654700.7406493165199415No Hit
TGAGGTAGTAGGTTGTGTGGT649400.7346535300871392No Hit
TCAGTGCACTACAGAACTTTGT641930.7262028650582649No Hit
TGAGGGGCAGAGAGCGAGACT616110.6969932036063864No Hit
TGAGGTAGTAGTTTGTGCTGTT609150.6891194916116119No Hit
AAGCTGCCAGTTGAAGAACTGT597870.6763586480338741No Hit
TGAGGTAGTAGGTTGTATAGTT594330.6723539152089458No Hit
TGAGGTAGTAGGTTGTATAGT577930.6538009156810292No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC544240.6156880770166686No Hit
AAACCGTTACCATTACTGAG519700.5879264545523348No Hit
TGAGGTAGTAGGTTGTGTGGTT507860.5745320939175461No Hit
TGAGGTAGTAGATTGTATAGTT485890.549677862232892No Hit
AAAAGCTGGGTTGAGAGGGCGA470760.5325615888879298No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG438840.4964511166360335No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC424120.47979866827926926No Hit
TCGTACCGTGAGTAATAATGCG408100.4616755553257799No Hit
TGAGGTAGTAGGTTGTGTGG395650.4475911136109895No Hit
TCCTGTACTGAGCTGCCCCGAGA372120.4209720844102652No Hit
TGAGGTAGTAGATTGTATAGT347610.393244400359702No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT346920.3924638168429786No Hit
TGGCTCAGTTCAGCAGGAAC329580.3728474136835838No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT308800.34933940574516253No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC304660.34465590464482265No Hit
TAGCTTATCAGACTGATGTTG287260.32497162465788665No Hit
TAGCTTATCAGACTGATGTTGA279690.3164078315831105No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA274880.3109663725752276No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT272050.307764848876203No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT262990.297515447917488No Hit
AAACCGTTACCATTACTG245180.27736734294235416No Hit
CGCGACCTCAGATCAGACGT239270.27068147542955No Hit
TGAGGTAGTAGTTTGTACAGT233080.2636788493882205No Hit
TATTGCACTTGTCCCGGCCTG224440.25390458622229367No Hit
TTCACAGTGGCTAAGTTCCG221920.251053759465565No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA212410.2402952823002914No Hit
TCCTGTACTGAGCTGCCCCGAGT211250.238982996967829No Hit
AAAAGCTGGGTTGAGAGGGCG210850.23853048478422126No Hit
TGTAAACATCCCCGACTGGAAGCT196610.2224210510477863No Hit
TGAGGTAGTAGGTTGTATAG190040.21498853843202945No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA189750.21466046709891382No Hit
CATTGCACTTGTCTCGGTCTGA189070.2138911963867807No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC183540.20763521544840394No Hit
CTAGACTGAAGCTCCTTGAGG182110.20601748439200632No Hit
TGGAGTGTGACAATGGTGTTT181620.20546315696708686No Hit
TGAGAACTGAATTCCATGGGTT167830.1898627994372106No Hit
GGCTGGTCCGATGGTAGTGGGTTATC153840.17403618581553046No Hit
CAACGGAATCCCAAAAGCAGCTG150370.17011064262273345No Hit
TTCAAGTAATCCAGGATAGGCC149860.16953368958863363No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT147810.16721456464764403No Hit
TGAGGGGCAGAGAGCGAGACTT145020.16405829216698017No Hit
TGAGGTAGTAGTTTGTGCTGT138600.1567954716200762No Hit
CATTGCACTTGTCTCGGTCTG135660.15346950707055942No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC135390.15316406134662422No Hit
AAGCTGCCAGTTGAAGAACT132470.14986072240628784No Hit
CGCGACCTCAGATC126110.14266577868692504No Hit
AAAAGCTGGGTTGAGAGGGCGT124680.14104804763052742No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA122080.13810671843707722No Hit
CCCATAAAGTAGAAAGCACT118450.1340001703708371No Hit
TGTAAACATCCCCGACTGGAAG110460.12496123950327286No Hit
TGAGGTAGTAGTTTGTACAGTT110000.12444085049212396No Hit
CGCGACCTCAGATCAGAC104270.11795861346194332No Hit
AGCTCGGTCTGAGGCCCCTCAGT103680.11729115799112193No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC102210.11562817571636355No Hit
GGCTGGTCCGATGGTAGTGGGTT101470.11479102817668926No Hit
TGTAAACATCCCCGACTGGAAGC101220.11450820806193443No Hit
TGAGGTAGTAGTTTGTGCTG99830.1129357282238976No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC99400.1124492776265193No Hit
TATTGCACTTGTCCCGGCCTGC97410.11019802951307087No Hit
TGTAAACATCCTTGACTGGAAGCT96540.10921381551372408No Hit
TACAGTACTGTGATAACTGAAG95970.10856898565208307No Hit
AAAAGCTGGGTTGAGAGGGCGAA95270.10777708933076954No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC91510.10352347480485696No Hit
CAACGGAATCCCAAAAGCAGCT90240.10208674862190242No Hit
GGCTGGTCCGATGGTAGTG90150.1019849333805907No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG90040.10186049253009856No Hit
TGAGGTAGTAGGTTGTGTGGC89590.10135141632353988No Hit
TGAGGGGCAGAGAGCGAGACC89340.10106859620878504No Hit
TGAGGTAGTAGATTGTATAG88840.1005029559792754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)