FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002167602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10503885
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT207513619.755890320581386No Hit
TCCTGTACTGAGCTGCCCCGAG3290563.132707564867666No Hit
TCCTGTACTGAGCTGCCCCGA3000512.8565716399218No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2717242.5868904695738766No Hit
AAACCGTTACCATTACTGAGC2202322.096671850462948No Hit
TATTGCACTTGTCCCGGCCTGT2150982.0477946969145226No Hit
AAACCGTTACCATTACTGAG1733151.6500085444575985No Hit
TCAGTGCACTACAGAACTTTGT1605751.5287200878532086No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1188401.1313909091731298No Hit
CGCGACCTCAGATCAGA1154741.0993456230718444No Hit
TCCTGTACTGAGCTGCCCCG880270.8380423052994203No Hit
TGAGGGGCAGAGAGCGAGACT848240.8075488259820056No Hit
TGAGGTAGTAGGTTGTATAGT837470.7972954768640366No Hit
TTCAAGTAATCCAGGATAGGCT831390.7915071423573278No Hit
CATTGCACTTGTCTCGGTCTGA789020.7511696862637015No Hit
TGAGGGGCAGAGAGCGAGACTTT730460.6954188854885597No Hit
TGAGGTAGTAGGTTGTATAGTT723100.6884119542435965No Hit
AAACCGTTACCATTACTG673450.641143729201148No Hit
TAGCTTATCAGACTGATGTTGAC632140.6018154235313886No Hit
TAGCTTATCAGACTGATGTTGA614180.5847169880477556No Hit
TCCTGTACTGAGCTGCCCCGAGA609480.5802424531494775No Hit
TGAGGTAGTAGATTGTATAGTT600610.5717979585648548No Hit
TGAGGTAGTAGATTGTATAGT591960.5635629102946196No Hit
TGAGGTAGTAGGTTGTATAG558360.5315747459154399No Hit
AAGCTGCCAGTTGAAGAACTGT547830.5215498836858934No Hit
AAAAGCTGGGTTGAGAGGGCGA544250.5181416209335878No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG537970.5121628806865269No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT468870.446377697394821No Hit
TGAGGTAGTAGGTTGTGTGG456540.43463918350210423No Hit
TAGCTTATCAGACTGATGTTG455390.4335443504950787No Hit
TGAGGTAGTAGTTTGTGCTGTT453230.4314879684992743No Hit
TGAGGTAGTAGGTTGTGTGGT447130.4256805934185304No Hit
TATTGCACTTGTCCCGGCCTG426760.4062877687636527No Hit
CCCATAAAGTAGAAAGCACT413570.39373051018742117No Hit
TGAGGTAGTAGGTTGTGTGGTT402660.3833438770512053No Hit
TGTAAACATCCCCGACTGGAAG396570.377546022257479No Hit
TGAGGTAGTAGTTTGTACAGT391480.372700196165514No Hit
CATTGCACTTGTCTCGGTCTG383840.36542669688405766No Hit
TCGTACCGTGAGTAATAATGCG346700.33006835090064296No Hit
TGAGGTAGTAGTTTGTGCTGT343560.3270789807771125No Hit
AAACCGTTACCATTACTGAGTT340120.3238040020430536No Hit
TGAGGTAGTAGATTGTATAG316890.30168837530113857No Hit
TCCTGTACTGAGCTGCCCCGAGT308000.2932248401424806No Hit
TACAGTACTGTGATAACTGAAG307370.292625062060371No Hit
TATTGCACTTGTCCCGGCCTGC269090.2561814033569484No Hit
CGCGACCTCAGATC267700.2548580834615002No Hit
TGGCTCAGTTCAGCAGGAAC267160.2543439879625491No Hit
TGAGGGGCAGAGAGCGAGACTT261370.24883174177935113No Hit
TAGCAGCACGTAAATATTGGCG256590.24428104458493216No Hit
TGTAAACATCCCCGACTGGAAGCT252620.2405014906389398No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC243160.23149529912027786No Hit
ACCAATATTACTGTGCTGCTT237340.2259544920760271No Hit
TGGAGTGTGACAATGGTGTTT225190.21438734334962728No Hit
TTCACAGTGGCTAAGTTCCG216080.20571436187658185No Hit
TGTAAACATCCTTGACTGGAAGCT206410.19650824433055009No Hit
AAAAGCTGGGTTGAGAGGGCG205650.19578470251721147No Hit
TGAGGTAGTAGTTTGTGCTG201390.19172906024770836No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC198560.18903481902172387No Hit
TCAGTGCACTACAGAACTTTGC184170.17533512600337875No Hit
CTAGACTGAAGCTCCTTGAGG184020.17519232169811455No Hit
AGACCGTTACCATTACTGAGT182560.17380235979354305No Hit
TGAGGTAGTAGTTTGTACAGTT176840.16835675561946842No Hit
TGTAAACATCCCCGACTGGAAGC169960.16180679815135068No Hit
TGTAAACATCCCCGACTGGAA168110.16004554505309226No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA167460.1594267263969474No Hit
TACAGTACTGTGATAACTGA158090.15050621746144402No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA157980.1504014943042503No Hit
CGCGACCTCAGATCAGACGT153660.14628873031264145No Hit
TGGAGAGAAAGGCAGTTCCTG145750.13875818328170958No Hit
AAACCGTTACCATTACTGGGT142640.13579737401923192No Hit
AAAAGCTGGGTTGAGAGGGCGAA142440.13560696827887966No Hit
AAGCTGCCAGTTGAAGAACT142400.13556888713080922No Hit
TGAGAACTGAATTCCATGGGTT141800.13499766990975243No Hit
AACATTCAACGCTGTCGGTGA138470.1318274143328873No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG138040.13141804199112994No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC134630.1281716241181239No Hit
AAACCGTTACTATTACTGAGT133840.12741952144373248No Hit
TAGCAGCACGTAAATATTGGC133190.12680070278758762No Hit
TTCAAGTAATTCAGGATAGGT132400.1260486001131962No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC127900.12176447095527036No Hit
AAACCGTTACCATTACTGAGG127000.12090764512368518No Hit
GAACCGTTACCATTACTGAGT126260.12020314388438182No Hit
AAACCGTTACCATTACTGAGA122210.11634742764224856No Hit
TAGCAGCACGTAAATATTGG121630.115795250995227No Hit
TGGCTCAGTTCAGCAGGAACAG117880.11222514336362212No Hit
TTCAAGTAATCCAGGATAGGCC114800.10929289496219732No Hit
TCCTGTACTGAGCTGCCCCGAA114740.10923577324009164No Hit
TCAGGCTCAGTCCCCTCCCGA110420.10512300924848282No Hit
AAAAGCTGGGTTGAGAGGGCGT108790.10357120246461189No Hit
TGAGGTAGTAGGTTGTAT108600.10339031701127725No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT107720.10255253175372732No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA106690.10157194219091317No Hit
ACCAATATTACTGTGCTGCTTT106590.10147673932073704No Hit
TGTAAACATCCCCGACTGGA106450.10134345530249046No Hit
TGGAGAGAAAGGCAGTTCCTGA106330.10122921185827911No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)