FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002167603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18933155
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT13696027.233881516313579No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC12870566.797895015384388No Hit
AAACCGTTACCATTACTGAGT10002025.282806801085186No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC3276541.730583201796003No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT2707281.4299148768390688No Hit
TCAGTGCACTACAGAACTTTGT2584191.3649019405376441No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC2255171.1911221346891208No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC2137321.1288768300898608No Hit
TATTGCACTTGTCCCGGCCTGT2109461.1141619027573588No Hit
TTCAAGTAATCCAGGATAGGCT1948491.0291417357540251No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT1694790.8951439947541759No Hit
TCCTGTACTGAGCTGCCCCGAG1682900.8888640060254088No Hit
AAACCGTTACCATTACTGAGC1606000.8482474262741735No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT1525900.8059406897582574No Hit
TGAGGTAGTAGGTTGTATAGTT1382000.729936452746518No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1340310.7079168791466609No Hit
TGAGGTAGTAGGTTGTATAGT1285190.6788039288750343No Hit
TGAGGTAGTAGATTGTATAGTT1137580.600840166364243No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT1132070.5979299276850583No Hit
TCCTGTACTGAGCTGCCCCGA1101130.5815882244665509No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT1078450.5696092383968757No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT1002010.5292356186805632No Hit
TAGCTTATCAGACTGATGTTGAC985350.5204362400244439No Hit
TGAGGGGCAGAGAGCGAGACTTT978270.5166967681825877No Hit
TGAGGTAGTAGATTGTATAGT900710.47573159359863687No Hit
TGAGGTAGTAGTTTGTGCTGTT898520.4745748925628085No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA887440.4687227247651012No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC871170.46012933396467726No Hit
TGAGGTAGTAGGTTGTGTGGTT785190.41471693439366025No Hit
CGCGACCTCAGATCAGA687050.3628819391168561No Hit
CATTGCACTTGTCTCGGTCTGA678420.35832379759210764No Hit
AAGCTGCCAGTTGAAGAACTGT678340.3582815436729906No Hit
TGAGGTAGTAGTTTGTACAGT642170.33917749049220797No Hit
TGAGGTAGTAGGTTGTGTGGT640000.3380313529361588No Hit
TCCTGTACTGAGCTGCCCCGAGA606950.32057520260094No Hit
TAGCTTATCAGACTGATGTTGA584430.3086807243694989No Hit
TCCTGTACTGAGCTGCCCCGAGT565150.29849752986229716No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT563290.2975151262428264No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC559320.2954182755066443No Hit
AAACCGTTACCATTACTGAG536380.28330196419983883No Hit
TGTAAACATCCCCGACTGGAAGCT500770.2644936884528754No Hit
TGAGGGGCAGAGAGCGAGACT474330.2505287681847003No Hit
TCAGTGCACTACAGAACTTTGC447250.23622581656358912No Hit
TCGTACCGTGAGTAATAATGCG435540.23004089915283532No Hit
TATTGCACTTGTCCCGGCCTGC434400.2294387808054178No Hit
CGCGACCTCAGATCAGACGT414720.21904431670263091No Hit
TGAGGTAGTAGTTTGTACAGTT397990.21020796586728413No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT395200.20873436043807808No Hit
CCCATAAAGTAGAAAGCACT393770.2079790716338613No Hit
AAACCGTTACCATTACTGAGTT393580.20787871857595844No Hit
TTCAAGTAATCCAGGATAGGCC390170.20607764527359546No Hit
TGGAGTGTGACAATGGTGTTT373940.19750538143273005No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT363020.19173772147325685No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA360390.1903486238822848No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC351850.18583801801654296No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCT348900.18427990474910283No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCC340300.1797376084440232No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG333270.17602454530161507No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA327400.17292416398640376No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC316490.16716178576682017No Hit
AAAAGCTGGGTTGAGAGGGCGA313550.1656089542392697No Hit
TGAGAACTGAATTCCATGGGTT312570.1650913437300862No Hit
TCCTGTACTGAGCTGCCCCG308020.16268815208030568No Hit
CGCGACCTCAGATC306380.16182194673840677No Hit
TGTAAACATCCTTGACTGGAAGCT306010.16162652236249056No Hit
TGAGGTAGTAGGTTGTATAG294060.15531484319438574No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG284010.1500066946053101No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT271050.1431615597083529No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC268260.14168795427914682No Hit
TGGCTCAGTTCAGCAGGAAC262640.13871961646117617No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC259760.13719847537296345No Hit
TAGCTTATCAGACTGATGTTG250560.13233927467450618No Hit
GCATTGGTGGTTCAGTGGTAGAATT248600.1313040536561392No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC247790.13087623272507937No Hit
TTCAAGTAATTCAGGATAGGT247050.13048538397324694No Hit
TGTAAACATCCCCGACTGGAAG245820.12983572996682274No Hit
TGAGGTAGTAGTTTGTGCTGT245290.12955579775267248No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAATT241240.12741669309737336No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG240400.12697302694664464No Hit
TGAGGGGCAGAGAGCGAGACTT239160.12631809120033083No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCC235920.12460680747609154No Hit
TGAGGTAGTAGGTTGTATAGTC221430.11695356637602132No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA219490.11592890883743358No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA212560.11226866309392175No Hit
TGAGGTAGTAGGTTGTATAGC210100.11096935508107339No Hit
AGCTCGGTCTGAGGCCCCTCAGT205620.10860313561052028No Hit
TTCACAGTGGCTAAGTTCCG205160.1083601755755974No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTC201360.10635311441753896No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA195630.10332667746078242No Hit
CATTGCACTTGTCTCGGTCTG193310.10210131380638886No Hit
TGTAAACATCCCCGACTGGAAGC189810.10025270484501922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)