FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167605

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167605
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12958046
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT165974112.808574687881183No Hit
TCAGTGCACTACAGAACTTTGT7312645.643319988214272No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC4942133.8139469484828195No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2742132.11616010623824No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1975681.5246743220389865No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1801631.3903562311786823No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG1490331.1501193930010745No Hit
AAACCGTTACCATTACTGAGC1447641.1171746110486103No Hit
TCCTGTACTGAGCTGCCCCGAG1409481.087725726548586No Hit
TAGCTTATCAGACTGATGTTGAC1334671.029993256699351No Hit
TCCTGTACTGAGCTGCCCCGA1294890.9992941837064013No Hit
TTCAAGTAATCCAGGATAGGCT1267640.9782647785013264No Hit
TTCACAGTGGCTAAGTTCCG1069170.8251012536921075No Hit
AAACCGTTACCATTACTGAG1056920.815647667865973No Hit
TCGTACCGTGAGTAATAATGCG974980.7524128252052817No Hit
TAGCTTATCAGACTGATGTTGA941740.72676080946155No Hit
CATTGCACTTGTCTCGGTCTGA888650.6857901260730206No Hit
TATTGCACTTGTCCCGGCCTGT829530.6401659632941571No Hit
AAGCTGCCAGTTGAAGAACTGT816560.6301567381378335No Hit
TGAGGGGCAGAGAGCGAGACT757970.5849415876436925No Hit
TGGCTCAGTTCAGCAGGAAC751660.5800720262916184No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT719590.555322924459444No Hit
TCAGTGCACTACAGAACTTTGC696720.5376736585130196No Hit
TGGAGTGTGACAATGGTGTTT686190.5295474333090036No Hit
TGAGGGGCAGAGAGCGAGACTTT645290.49798403246909295No Hit
TGAGGTAGTAGGTTGTGTGG622010.48001836079297755No Hit
CGCGACCTCAGATCAGA620740.4790382747522273No Hit
TGAGGTAGTAGTTTGTGCTGTT616770.47597454122326777No Hit
TGGCTCAGTTCAGCAGGAACAG610660.47125932412957944No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC609500.47036412743094136No Hit
TGAGATGAAGCACTGTAGCTC585210.4516190172499774No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC584700.4512254393910934No Hit
AAACCGTTACCATTACTG579710.4473745501443659No Hit
TAGCTTATCAGACTGATGTTG577600.445746218218395No Hit
TGAGGTAGTAGGTTGTATAGT566830.4374347799043158No Hit
CCCATAAAGTAGAAAGCACT560350.43243402593261365No Hit
TGAGGTAGTAGGTTGTGTGGT533300.4115589649859246No Hit
AAAAGCTGGGTTGAGAGGGCGA528800.40808621917224247No Hit
TGAGGTAGTAGGTTGTATAGTT517510.3993734857863601No Hit
AAATGGATTTTTGGAGCAGGGAGA516080.3982699243389011No Hit
CTAGACTGAAGCTCCTTGAGG505360.3899970720894184No Hit
TGAGGTAGTAGGTTGTGTGGTT466500.3600079826850437No Hit
TCCTGTACTGAGCTGCCCCG457230.3528541263088586No Hit
CGCGACCTCAGATCAGACGT442050.341139397097371No Hit
TGAGGTAGTAGATTGTATAGT412640.31844307390172866No Hit
CATTGCACTTGTCTCGGTCTG403860.311667360958589No Hit
AGGAAACTCTGGTG384620.2968194433018682No Hit
TGAGGTAGTAGGTTGTATAG378850.2923666114474358No Hit
TGAGGTAGTAGATTGTATAGTT364840.28155479614750556No Hit
TGGCTCAGTTCAGCAGGAACA347270.26799565304830686No Hit
TGAGATGAAGCACTGTAGCT337600.26053310815534997No Hit
CGTGTATTTGACAAGCTGAGTTG334420.25807903444701463No Hit
TGAGGTAGTAGTTTGTACAGT330410.25498443206637794No Hit
TAGCAGCACGTAAATATTGGCG314330.24257515369215388No Hit
TACAGTACTGTGATAACTGAAG307880.23759755135920957No Hit
TCCTGTACTGAGCTGCCCCGAGA304240.2347884858565867No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC301980.2330443957368264No Hit
TGTAAACATCCCCGACTGGAAG279000.2153102404482898No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC265050.20454472842587534No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG264500.20412028171531418No Hit
TGAGGTAGTAGTTTGTGCTGT247120.19070776566158198No Hit
AAAAGCTGGGTTGAGAGGGCG243260.1877289214747347No Hit
AAATGGATTTTTGGAAATAGGAGA240450.1855603846444132No Hit
AAACCGTTACCATTACTGAGTT239090.1845108436873893No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT235300.18158601999097704No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA223610.1725645980883229No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA221450.17089768009775547No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC221410.1708668112460783No Hit
TGAGGTAGTAGATTGTATAG209910.16199201638889074No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT209160.1614132254199437No Hit
TTCACAGTGGCTAAGTTCCGA207070.15980032791981136No Hit
TGTAAACATCCTTGACTGGAAGCT204320.15767809436700564No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC199290.153796336268601No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT193250.1491351396653477No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA190300.1468585618541561No Hit
TTCAAGTAATTCAGGATAGGT180960.13965068498753594No Hit
TGAGAACTGAATTCCATGGGTT180780.13951177515498867No Hit
TCCTGTACTGAGCTGCCCCGAGT178610.13783713995150196No Hit
TACAGTACTGTGATAACTGA176390.13612391868341878No Hit
TGAGGGGCAGAGAGCGAGACTT174690.13481199248713888No Hit
TGAGGTAGTAGTTTGTACAGTT172510.13312964007073289No Hit
GGCTGGTCCGATGGTAGTGGGTTATC171900.132658890082656No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA171270.1321727056687405No Hit
TCGTACCGTGAGTAATAATGC167940.12960287376661575No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG162500.12540470993852004No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG160240.12366061981875971No Hit
TATTGCACTTGTCCCGGCCTG159730.12326704195987574No Hit
TGAGGTAGTAGTTTGTGCTG155180.11975571008159717No Hit
TAGCAGCACGTAAATATTGGC154820.11947789041650261No Hit
ACGGCCCTGGCGGAGC153410.11838976339488222No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT151500.11691577572729717No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGCC151000.11652991508133248No Hit
TCAGTGCATCACAGAACTTTGT150640.1162520954162379No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT147550.1138674766241762No Hit
CTAGACTGAAGCTCCTTGAGGA144320.1113748168512444No Hit
AGACCGTTACCATTACTGAGT143870.11102754226987618No Hit
AAATGGATTTTTGGAGCAGGGAGAT143520.1107574398177009No Hit
TTCAAGTAATCCAGGATAGGCC140170.10817217348973757No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT139190.10741588662364682No Hit
AAATGGATTTTTGGAGCAGGGAG137690.10625830468575279No Hit
AAAAGCTGGGTTGAGAGGGCGT134800.10402803015207696No Hit
AAACCGTTACTATTACTGAGT134010.10341837033145274No Hit
TACCACAGGGTAGAACCACGGA132140.10197525151554486No Hit
TACAGTACTGTGATAACTGAA130890.10101059990063317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)