FastQCFastQC Report
Wed 8 May 2024
EGAF00002167606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13555442
Total Bases316.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT165366112.199240718229623No Hit
TCAGTGCACTACAGAACTTTGT3253852.400401255820356No Hit
AAACCGTTACCATTACTGAGC3065562.261497633201485No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1732201.277863163738962No Hit
TTCAAGTAATCCAGGATAGGCT1633751.2052355061531745No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1599611.1800500492717243No Hit
TGAGGTAGTAGGTTGTATAGT1166990.8609014741090699No Hit
TGGAGTGTGACAATGGTGTTT1128220.8323004148444588No Hit
TGAGGTAGTAGGTTGTGTGGT1023220.7548407495675906No Hit
TATTGCACTTGTCCCGGCCTGT982210.7245872174437396No Hit
TAGCTTATCAGACTGATGTTGAC967890.7140231945221706No Hit
TGAGGTAGTAGTTTGTGCTGTT916330.6759868103157388No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC896570.6614096390217302No Hit
TCCTGTACTGAGCTGCCCCGAG889670.6563194324463931No Hit
AAGCTGCCAGTTGAAGAACTGT820000.6049231002574464No Hit
TGAGGTAGTAGGTTGTGTGGTT796500.5875868894573855No Hit
TGAGGTAGTAGGTTGTATAGTT793590.5854401501625693No Hit
TGAGGTAGTAGATTGTATAGT781560.576565485655134No Hit
TCCTGTACTGAGCTGCCCCGA677740.4999763194737582No Hit
TCAGTGCACTACAGAACTTTGC657140.4847794708575346No Hit
TAGCTTATCAGACTGATGTTGA616990.455160370277856No Hit
TGAGGTAGTAGATTGTATAGTT595390.43922581056375737No Hit
TGAGGGGCAGAGAGCGAGACTTT560090.41318460880877217No Hit
TCGTACCGTGAGTAATAATGCG551090.4065452089278977No Hit
AAACCGTTACCATTACTGAG549980.4057263496092566No Hit
TGAGGTAGTAGTTTGTACAGT537880.3968000453249698No Hit
CCCATAAAGTAGAAAGCACT503540.3714670462239446No Hit
TGAGGGGCAGAGAGCGAGACT485360.3580554584645783No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT472910.34887095529603535No Hit
CGGACAGGATTGACAGA446660.32950603897681835No Hit
CATTGCACTTGTCTCGGTCTGA395390.29168359098877045No Hit
CGCGACCTCAGATCAGA380100.28040398830226265No Hit
TAGCTTATCAGACTGATGTTG376370.27765232590718913No Hit
TTCACAGTGGCTAAGTTCCG374710.2764277254847168No Hit
TTCAAGTAATCCAGGATAGGCC369870.2728572037710021No Hit
TTCAAGTAATTCAGGATAGGT362760.2676120778651113No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC352330.25991775111427573No Hit
AAACCGTTACCATTACTGAGA331690.24469139405413706No Hit
TACAGTATAGATGATGTACT327760.24179218943948858No Hit
AAACCGTTACCATTACTG316930.23380277824950305No Hit
TCCTGTACTGAGCTGCCCCGAGA303680.22402810620266014No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG302680.2232903951047852No Hit
TCCTGTACTGAGCTGCCCCGAGT301350.22230923934461155No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT300840.22193300668469534No Hit
TGGCTCAGTTCAGCAGGAAC297980.219823152944773No Hit
TGAGGTAGTAGGTTGTATAG292710.21593541545897213No Hit
TGAGGTAGTAGGTTGTGTGG280980.20728206428089913No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC280670.2070533738405579No Hit
CGCGACCTCAGATCAGACGT263980.19474097561702525No Hit
TGTAAACATCCCCGACTGGAAGCT236400.17439490353763457No Hit
TATTGCACTTGTCCCGGCCTGC233260.17207849069030726No Hit
TCCTGTACTGAGCTGCCCCG233170.1720120966914985No Hit
TGAGATGAAGCACTGTAGCTC232210.17130389403753857No Hit
TGAGGTAGTAGTTTGTGCTGT226550.16712844922356646No Hit
TGAGGTAGTAGGTTGTATAGC222940.16446531216023794No Hit
TGAGATGAAGCACTGTAGCT222670.1642661301638117No Hit
TGAGGTAGTAGGTTGTGTGGC217830.160695608450097No Hit
TGGAGTGTGACAATGGTGTTC208170.15356931924462516No Hit
TAGCTTATCAGACTGATGTTGAT207520.15308980703100644No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT206130.15206438860496027No Hit
CTAGACTGAAGCTCCTTGAGG204540.15089142795933913No Hit
TGTAAACATCCTTGACTGGAAGCT203670.15024961930418795No Hit
TGAGGTAGTAGTTTGTACAGTT200760.14810288000937188No Hit
AAACCGTTACCATTACTGAGTT197370.14560203938757585No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC196830.1452036753947234No Hit
TGAGGTAGTAGTTTGTGCTGTC187210.13810689463316653No Hit
TTCAAGTAATCCAGGATAGGCA182050.13430030536813184No Hit
TGAGAACTGAATTCCATGGGTT181620.13398308959604563No Hit
AAACCGTTACCATTACTGAGG180760.13334865805187318No Hit
AAAAGCTGGGTTGAGAGGGCGA179720.13258143851008325No Hit
TACAGTACTGTGATAACTGAAG173380.12790435014955617No Hit
TGAGGTAGTAGGTTGTGTGGTC173150.12773467659704493No Hit
AAGCTGCCAGTTGAAGAACTGC172470.12723303305049No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC170920.12608958084878383No Hit
TGAGGTAGTAGATTGTATAG169310.1249018659812052No Hit
TGAGGTAGTAGGTTGTATAGTC168870.12457727309814022No Hit
CATTGCACTTGTCTCGGTCTG168660.12442235376758648No Hit
TGGCTCAGTTCAGCAGGAACAG163520.1206305187245093No Hit
TCGTACCGTGAGTAATAATGCA157740.1163665485787922No Hit
TGAGGTAGTAGTTTGTGCT155890.11500178304772357No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT154360.11387308506797492No Hit
GGTGGGCGTAGCTC153930.11355586929588869No Hit
GTCTGAGCGTCGCT151810.11199192176839384No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT150490.11101814311919893No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA148800.10977141136379027No Hit
TGAGGTAGTAGATTGTATAGC147600.10888615804634035No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT146250.1078902480642092No Hit
AAACCGTTACTATTACTGAGT142360.1050205518934757No Hit
TGAGGTAGTAGGTTGTAT141750.10457054812377199No Hit
TGAGGGGCAGAGAGCGAGACTT139340.1027926643778934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)