FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9880515
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8426518.528411727526349No Hit
TCAGTGCACTACAGAACTTTGT3552593.5955514464580034No Hit
AAACCGTTACCATTACTGAGC1710471.7311547019563251No Hit
CGCGACCTCAGATCAGA1523261.5416807727127584No Hit
TATTGCACTTGTCCCGGCCTGT1483941.50188527622295No Hit
TCCTGTACTGAGCTGCCCCGAG1264361.279649896791817No Hit
TCCTGTACTGAGCTGCCCCGA1198931.2134286522514262No Hit
TGAGGTAGTAGGTTGTATAGT1195821.2102810430427968No Hit
TTCAAGTAATCCAGGATAGGCT957840.9694231525381016No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC934130.9454264276710274No Hit
TGAGGTAGTAGGTTGTATAGTT903550.9144766239411609No Hit
TGAGGGGCAGAGAGCGAGACTTT813030.8228619662031786No Hit
TGAGGTAGTAGATTGTATAGT797820.8074680317776959No Hit
TCAGTGCACTACAGAACTTTGC769230.7785322931041551No Hit
TGAGGTAGTAGATTGTATAGTT666800.6748636078180136No Hit
AAGCTGCCAGTTGAAGAACTGT665980.6740336915636482No Hit
CATTGCACTTGTCTCGGTCTGA664320.6723536171950552No Hit
TGAGGTAGTAGGTTGTGTGGTT646040.653852557280668No Hit
TGAGGTAGTAGGTTGTGTGGT639820.6475573388634095No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT525190.5315411190611016No Hit
TGAGGGGCAGAGAGCGAGACT502370.5084451569579116No Hit
TGAGGTAGTAGTTTGTGCTGTT498700.5047307756731304No Hit
TGAGGTAGTAGTTTGTACAGT489440.49535879455676146No Hit
TGGAGTGTGACAATGGTGTTT484680.49054123190947035No Hit
CCCATAAAGTAGAAAGCACT462710.4683055488504395No Hit
CGCGACCTCAGATCAGACGT432780.4380136055661067No Hit
TCCTGTACTGAGCTGCCCCGAGT422840.4279534012144104No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT413300.41829803405996546No Hit
TCCTGTACTGAGCTGCCCCGAGA412260.41724545734711194No Hit
TAGCTTATCAGACTGATGTTGA381290.3859009373499256No Hit
TATTGCACTTGTCCCGGCCTGC380070.384666183898309No Hit
AAACCGTTACCATTACTGAG347610.3518136453413612No Hit
TAGCTTATCAGACTGATGTTGAC342580.34672281758592544No Hit
TGGCTCAGTTCAGCAGGAAC331780.33579221326013875No Hit
TCCTGTACTGAGCTGCCCCG320050.3239203624507427No Hit
CGCGACCTCAGATC310850.3146091069139615No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC276630.2799752846891078No Hit
AAATGGATTTTTGGAGCAGGGAGA274400.2777183173144315No Hit
TTCACAGTGGCTAAGTTCCG268540.2717874523746991No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT256580.2596828201768835No Hit
TGAGGTAGTAGGTTGTATAGC250360.2533876017596249No Hit
AAACCGTTACCATTACTGAGTT247610.2506043460285218No Hit
AAACCGTTACCATTACTGAGA241500.2444204578405073No Hit
TGAGGTAGTAGGTTGTATAG231440.23423880232963565No Hit
TTCAAGTAATCCAGGATAGGCC226880.22962365828097017No Hit
TGAGGTAGTAGTTTGTACAGTT225660.22838890482935353No Hit
TCCTGTACTGAGCTGCCCCGAA225290.22801443042189604No Hit
TGAGATGAAGCACTGTAGCT218830.2214763096862866No Hit
AAAAGCTGGGTTGAGAGGGCGA218480.22112207713869164No Hit
TAGCTTATCAGACTGATGTTG215390.2179947097899249No Hit
TGAGGGGCAGAGAGCGAGACTT215270.21787325863074952No Hit
TGAGGTAGTAGGTTGTATAGTC212120.2146851657023951No Hit
AAATGGATTTTTGGAGCAGGGA212080.21464468198266995No Hit
TGAGGTAGTAGGTTGTGTGG198390.20078912890674222No Hit
CATTGCACTTGTCTCGGTCTG197070.1994531661558127No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC193760.19610313834855772No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT193400.19573878487103152No Hit
AAATGGATTTTTGGAGCAGGG181360.18355318523376565No Hit
TCGTACCGTGAGTAATAATGCG177540.17968699000001517No Hit
AAATGGATTTTTGGAGCAGGGAG174070.17617502731385964No Hit
TGAGGTAGTAGTTTGTGCTGT170240.1722987111501779No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG168800.17084129724007302No Hit
TTCAAGTAATTCAGGATAGGT167720.16974823680749435No Hit
TGAGGTAGTAGATTGTATAGC163580.1655581718159428No Hit
TGTAAACATCCCCGACTGGAAG162980.16495091602006576No Hit
TCAGTGCACTACAGAACTTTGA159420.1613478649645287No Hit
TGAGGGGCAGAGAGCGAGACTTC157570.15947549292724114No Hit
AAACCGTTACCATTACTG157220.15912126037964622No Hit
TGAGGTAGTAGGTTGTGTGGTC155340.15721852555256483No Hit
TGAGGTAGTAGATTGTATAGTC153290.15514373491665162No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT151810.15364583728682157No Hit
TGTAAACATCCCCGACTGGAAGCT151210.15303858149094454No Hit
TGTAAACATCCTTGACTGGAAGCT147840.14962782810410186No Hit
AAGCTGCCAGTTGAAGAACTGC146900.14867646069056117No Hit
TGAGGTAGTAGGTTGTGTGGC141530.14324152131746168No Hit
GGAATACCGGGTGCTGTAGGCTT139130.14081249813395355No Hit
TGAGGTAGTAGGTTGTAT137140.13879843307762804No Hit
AAACCGTTACCATTACTGAGG130540.13211861932298063No Hit
TGAGGTAGTAGATTGTATAG130260.13183523328490468No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC124320.12582340090572203No Hit
TGAGATGAAGCACTGTAGCTC123470.12496312186156289No Hit
AAGCTGCCAGTTGAAGAACT122980.12446719629492997No Hit
TAGCTTATCAGACTGATGTTGAT121780.12325268470317591No Hit
TGAGAACTGAATTCCATGGGTT121510.12297941959503124No Hit
TGAGGTAGTAGATTGTAT117860.1192852801701126No Hit
TGAGGGGCAGAGAGCGAGACC116870.11828330810691548No Hit
AGCTCGGTCTGAGGCCCCTCAGT116440.11784810811987026No Hit
AAATGGATTTTTGGAGCAGGGAGAT115790.11719024767433682No Hit
CGTGTATTTGACAAGCTGAGTTG114600.11598585701251402No Hit
CGCGACCTCAGATCAGAC114170.11555065702546882No Hit
TACAGTACTGTGATAACTGAAG113950.11532799656698058No Hit
TTCAAGTAATCCAGGATAGGCA113490.1148624337901415No Hit
TGAGGTAGTAGTTTGTGCTGTC112110.11346574545962433No Hit
TATTGCACTTGTCCCGGCCTG109830.1111581734352916No Hit
TATTGCACTTGTCCCGGCCTGA107500.10879999676130243No Hit
TGGCTCAGTTCAGCAGGAACAG107290.10858745723274546No Hit
GCATTGGTGGTTCAGTGGTAGAATT106200.10748427587023551No Hit
CCCATAAAGTAGAAAGCACC102940.10418485271263694No Hit
TCAGTGCATCACAGAACTTTGT102430.10366868528614145No Hit
TGAGGTAGTAGTTTGTACAGC100960.10218090858624272No Hit
TGGAGTGTGACAATGGTGTTC100820.10203921556720474No Hit
TCCTGTACTGAGCTGCCCCA100580.10179631324885392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)