FastQCFastQC Report
Thu 9 May 2024
EGAF00002167609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14481889
Total Bases343.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT10239897.070824807454332No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6419074.432481149385968No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC5796924.002875591713209No Hit
TCCTGTACTGAGCTGCCCCGAG3629342.5061233379153784No Hit
TCCTGTACTGAGCTGCCCCGA3335852.3034633120030126No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2562021.7691200367576356No Hit
TATTGCACTTGTCCCGGCCTGT2472161.7070701204794485No Hit
CGCGACCTCAGATCAGA1944531.3427322913467987No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC1538141.0621128224363547No Hit
TGAGGTAGTAGGTTGTATAGT1408410.9725319673421057No Hit
TCAGTGCACTACAGAACTTTGT1379780.952762446943213No Hit
TCCTGTACTGAGCTGCCCCGAGA1350240.9323645554802967No Hit
AAACCGTTACCATTACTGAGC1078620.7448061506340782No Hit
TGAGGTAGTAGGTTGTATAGTT1040990.718821971360228No Hit
TTCAAGTAATCCAGGATAGGCT976490.6742835827563656No Hit
TGAGGTAGTAGGTTGTGTGGTT966250.6672126819919694No Hit
TCCTGTACTGAGCTGCCCCG931520.6432310039111611No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC916520.6328732391195652No Hit
TGAGGTAGTAGATTGTATAGT909210.627825555077794No Hit
TAGCTTATCAGACTGATGTTGAC891750.6157691168603765No Hit
TGAGGTAGTAGGTTGTGTGGT887430.6127860806003969No Hit
TGAGGTAGTAGATTGTATAGTT851900.58825198839737No Hit
TCCTGTACTGAGCTGCCCCGAGT736380.508483389149026No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG736360.5084695787959707No Hit
TGAGGGGCAGAGAGCGAGACTTT734140.5069366296068144No Hit
CATTGCACTTGTCTCGGTCTGA698440.4822851494028162No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT623340.43042727367955935No Hit
TAGCTTATCAGACTGATGTTGA619370.4276859185980503No Hit
TGAGGTAGTAGTTTGTGCTGTT611130.42199605313920024No Hit
AAGCTGCCAGTTGAAGAACTGT605060.4178046109868678No Hit
AAACCGTTACCATTACTGAG585630.4043878529934873No Hit
TGAGGTAGTAGGTTGTATAG580000.4005002386083749No Hit
TGAGGTAGTAGTTTGTACAGT531680.36713442562638066No Hit
TCGTACCGTGAGTAATAATGCG502310.3468539221644359No Hit
TGAGGGGCAGAGAGCGAGACT498140.3439744635523722No Hit
TATTGCACTTGTCCCGGCCTG495910.3424346091866883No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC488780.3375112183224164No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT481450.3324497239275898No Hit
TGGAGTGTGACAATGGTGTTT418050.2886709047417778No Hit
TGAGGTAGTAGGTTGTGTGG414580.2862748084866553No Hit
TAGCTTATCAGACTGATGTTG405480.27999109784642046No Hit
AAACCGTTACCATTACTG402600.278002407006434No Hit
TGTAAACATCCCCGACTGGAAGCT344080.23759331396615455No Hit
CCCATAAAGTAGAAAGCACT331770.2290930416605182No Hit
CATTGCACTTGTCTCGGTCTG325490.22475659080110336No Hit
TGAGGTAGTAGATTGTATAG323660.22349294349652865No Hit
TGTAAACATCCCCGACTGGAAG322090.22240883078167495No Hit
AAAAGCTGGGTTGAGAGGGCGA314250.21699517238393415No Hit
TACAGTACTGTGATAACTGAAG295260.20388224215777376No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT294330.20324006074069484No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC290210.20039512801126982No Hit
TGGCTCAGTTCAGCAGGAAC279460.1929720632439594No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT273290.18871156932634964No Hit
TATTGCACTTGTCCCGGCCTGC269550.18612903330497838No Hit
TGTAAACATCCCCGACTGGAAGC257960.1781259337093386No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC249270.1721253353067407No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC242070.1671536082067747No Hit
CTAGACTGAAGCTCCTTGAGG241440.16671858208552767No Hit
CGCGACCTCAGATC230950.15947505190793826No Hit
TATTGCACTTGTCCCGGCCTGTA216080.14920705441120286No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG192820.13314561380770146No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG191390.132158173564236No Hit
TTCACAGTGGCTAAGTTCCG189120.13059069849244115No Hit
TGAGGTAGTAGTTTGTGCTGT188990.13050093119758066No Hit
GCATTGGTGGTTCAGTGGTAGAATT188040.12984493942744624No Hit
TGAGGTAGTAGTTTGTACAGTT186690.1289127405962026No Hit
TGGCTCAGTTCAGCAGGAACAG186300.12864343871162112No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC184440.12735907587746323No Hit
TTCAAGTAATTCAGGATAGGT183130.12645449775233053No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA178100.12298119395888203No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT176770.12206280548069386No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA176630.12196613300930563No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC176500.12187636571444511No Hit
TGAGGTAGTAGGTTGTAT170840.1179680357997496No Hit
TGAGGGGCAGAGAGCGAGACTT165280.1141287576503314No Hit
CGCGACCTCAGATCAGACGT164870.11384564541269443No Hit
CGCGACCTCAGATCAGAC163420.11284439481617349No Hit
TGAGGTAGTAGGTTGTATAGC157680.10888082348925612No Hit
TGAGAACTGAATTCCATGGGTT156800.10827316795481584No Hit
TGTAAACATCCTTGACTGGAAGCT155180.10715452935732349No Hit
ATACTAATAGCTCGAGGACTTATCC154170.10645710652802268No Hit
TCAGTGCACTACAGAACTTTGC153400.10592540793538743No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT148620.10262473355513221No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA148580.10259711284902129No Hit
AAGCTGCCAGTTGAAGAACT147560.10189278484319275No Hit
TGAGGTAGTAGATTGTAT146520.10117464648430878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)