FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12277456
Sequences flagged as poor quality0
Sequence length14-50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT186271415.171823869700694No Hit
TCAGTGCACTACAGAACTTTGT3360922.7374726490569383No Hit
AAACCGTTACCATTACTGAGC3046752.4815808747349615No Hit
TGAGGTAGTAGGTTGTATAGT2536832.0662505326836436No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2142201.7448240091432623No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2035351.657794578942087No Hit
TGAGGTAGTAGGTTGTATAGTT1836271.4956437229341324No Hit
TCCTGTACTGAGCTGCCCCGAG1778781.4488180613312724No Hit
TTCAAGTAATCCAGGATAGGCT1676041.3651362301766752No Hit
TGAGGTAGTAGATTGTATAGT1563971.2738551048360507No Hit
TATTGCACTTGTCCCGGCCTGT1524671.2418452161424973No Hit
TGAGGTAGTAGATTGTATAGTT1274251.037877879586781No Hit
TCCTGTACTGAGCTGCCCCGA1263211.0288857887171414No Hit
TGAGGTAGTAGGTTGTGTGGT1144020.9318054163663874No Hit
CGCGACCTCAGATCAGA1100030.8959755180552063No Hit
AAACCGTTACCATTACTGAG1088980.8869752821757211No Hit
TGAGGTAGTAGGTTGTGTGGTT890730.7255004619849584No Hit
TGAGGTAGTAGTTTGTGCTGTT863710.7034926453819097No Hit
TGAGGTAGTAGTTTGTACAGT851560.693596458419399No Hit
TGAGGGGCAGAGAGCGAGACTTT842820.6864777198142678No Hit
TGAGGGGCAGAGAGCGAGACT797750.6497681604397523No Hit
CATTGCACTTGTCTCGGTCTGA762850.6213420760783015No Hit
AAGCTGCCAGTTGAAGAACTGT759190.6183610024747798No Hit
CGCGACCTCAGATCAGACGT689690.5617531840472488No Hit
TCAGTGCACTACAGAACTTTGC583480.4752450344761977No Hit
TAGCTTATCAGACTGATGTTGAC555510.45246344193780863No Hit
TGAGGTAGTAGTTTGTACAGTT553730.4510136301852762No Hit
CCCATAAAGTAGAAAGCACT519300.4229703612865727No Hit
TCCTGTACTGAGCTGCCCCGAGA516420.42062459845101463No Hit
AAATGGATTTTTGGAGCAGGGA498830.40629752613244957No Hit
TGAGGTAGTAGGTTGTATAG496090.4040657934347311No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT490430.39945571786207174No Hit
AAACCGTTACCATTACTGAGTT468190.3813412159652619No Hit
TGAGGTAGTAGGTTGTATAGC417800.3402985113528405No Hit
TCCTGTACTGAGCTGCCCCGAGT406210.33085844494168826No Hit
TAGCTTATCAGACTGATGTTGA386990.3152037360182761No Hit
TCCTGTACTGAGCTGCCCCG386300.3146417303389236No Hit
AAATGGATTTTTGGAGCAGGGAGA385590.3140634346398798No Hit
TCGTACCGTGAGTAATAATGCG385000.3135828790589842No Hit
AAAAGCTGGGTTGAGAGGGCGA378980.3086795831318801No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC370190.3015201194775204No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC369550.30099883884739637No Hit
AAATGGATTTTTGGAGCAGGG365020.2973091493872998No Hit
AAATGGATTTTTGGAGCAGGGAG361660.29457242607914863No Hit
TTCAAGTAATCCAGGATAGGCC342110.2786489318308288No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT334390.27236098422995775No Hit
TATTGCACTTGTCCCGGCCTGC330190.26894008009476883No Hit
TTCAAGTAATTCAGGATAGGT311400.25363560659472123No Hit
TGAGGTAGTAGGTTGTATAGTC305380.24873231066761714No Hit
AAACCGTTACCATTACTGAGA282020.2297055676680902No Hit
AAACCGTTACCATTACTG276880.2255190326074066No Hit
TGGAGTGTGACAATGGTGTTT273840.2230429496143175No Hit
TGGCTCAGTTCAGCAGGAAC262440.21375763839023332No Hit
TGTAAACATCCCCGACTGGAAGCT262330.2136680432819307No Hit
TGAGGTAGTAGGTTGTGTGG252620.20575923872176777No Hit
AGAGGTAGTAGGTTGCATAGT252330.20552303343624284No Hit
TGAGGTAGTAGATTGTATAGC251390.20475740251074814No Hit
TGAGGTAGTAGATTGTATAG245650.2000821668593233No Hit
TGAGGTAGTAGTTTGTGCTGT243770.19855090500833397No Hit
TGAGGGGCAGAGAGCGAGACTT243020.1979400292699074No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT238260.19406300458335993No Hit
TTCACAGTGGCTAAGTTCCG237540.1934765638744704No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT236860.19292270320496363No Hit
CAACGGAATCCCAAAAGCAGCT234670.19113894604875797No Hit
CGCGACCTCAGATC229440.18687910589946322No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT226990.1848835784872697No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT225890.18398762740424401No Hit
TCCTGTACTGAGCTGCCCCGAA220660.17972778725494923No Hit
CATTGCACTTGTCTCGGTCTG219010.17838386063041073No Hit
TGAGGTAGTAGGTTGTGTGGC215150.1752398868299752No Hit
TGAGGTAGTAGGTTGTAT210890.17177011263571215No Hit
TGAGGTAGTAGATTGTATAGTC208600.16990490538104963No Hit
TGAGAACTGAATTCCATGGGTT204120.16625594097018145No Hit
AGAGGTAGTAGGTTGCATAGTT202690.16509120456224807No Hit
TAGCTTATCAGACTGATGTTG201520.1641382384103026No Hit
AAATGGATTTTTGGAGCAGGGAGAT196690.16020419865483534No Hit
AAGCTGCCAGTTGAAGAACT195080.1588928520696796No Hit
GGAATACCGGGTGCTGTAGGCTT190470.15513800253081747No Hit
AAACCGTTACCATTACTGAGG176980.1441503842489845No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT174360.14201639166941427No Hit
TGTAAACATCCTTGACTGGAAGCT174070.1417801863838893No Hit
TGAGGTAGTAGGTTGTGTGGTC168450.1372026908506127No Hit
TGAGGTAGTAGATTGTAT164210.13374920667604104No Hit
CTAGACTGAAGCTCCTTGAGG159380.12981516692057377No Hit
TGAGATGAAGCACTGTAGCT157960.1286585755224861No Hit
TATTGCACTTGTCCCGGCCTG151070.12304666373880713No Hit
AGACCGTTACCATTACTGAGT151050.12303037371911577No Hit
AGCTCGGTCTGAGGCCCCTCAGT148390.12086380110016277No Hit
TGAGGTAGTAGTTTGTGCTGTC145860.11880311360920373No Hit
AAACCGTTACTATTACTGAGT143620.11697863140376964No Hit
TGAGGTAGTAGTTTGTACAGC142930.11641662572441719No Hit
TGAGGGGCAGAGAGCGAGACC142910.11640033570472579No Hit
TGTAAACATCCCCGACTGGAAG138810.11306088166799376No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA137350.11187171023052332No Hit
TACAGTATAGATGATGTACT136720.11135857461024498No Hit
AAGCTGCCAGTTGAAGAACTGC136500.11117938439363986No Hit
TTCAAGTAATCCAGGATAGGCA134990.10994948790694098No Hit
TGAGGTAGTAGTTTGTGCT132830.1081901657802724No Hit
AAAAGCTGGGTTGAGAGGGCG128740.10485885675338605No Hit
CGCGACCTCAGATCAGACGC128140.10437015616264478No Hit
AAAAGCTGGGTTGAGAGGGCGT127700.1040117757294345No Hit
TGGCTCAGTTCAGCAGGAACAG124420.10134021250004888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)