FastQCFastQC Report
Wed 8 May 2024
EGAF00002167614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24961254
Total Bases545.2 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT22975849.20460165983648No Hit
TCAGTGCACTACAGAACTTTGT7357252.9474681039662514No Hit
TTCAAGTAATCCAGGATAGGCT4292001.719464895473601No Hit
AAACCGTTACCATTACTGAGC4129101.6542037511416692No Hit
CGCGACCTCAGATCAGA3505051.4041962795619163No Hit
TAGCTTATCAGACTGATGTTGAC2863811.1473021347405064No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2285590.9156551189295217No Hit
AAGCTGCCAGTTGAAGAACTGT2206460.8839539872475958No Hit
TGAGGTAGTAGGTTGTATAGT2143360.8586748085653068No Hit
AAACCGTTACCATTACTGAG2034940.8152394907723787No Hit
TATTGCACTTGTCCCGGCCTGT1996870.7999878531743637No Hit
TGAGGTAGTAGATTGTATAGTT1950580.7814431117923802No Hit
TGAGGTAGTAGGTTGTATAGTT1917990.7683868767170111No Hit
TAGCTTATCAGACTGATGTTGA1895640.7594329996401623No Hit
TCCTGTACTGAGCTGCCCCGAG1755540.7033060117893115No Hit
TGAGGTAGTAGATTGTATAGT1751210.7015713232997027No Hit
TGAGGTAGTAGTTTGTGCTGTT1555510.6231698135037607No Hit
TCAGTGCACTACAGAACTTTGC1405620.5631207470586214No Hit
TCCTGTACTGAGCTGCCCCGA1383980.5544513108195606No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1324730.5307145225956997No Hit
TAGCTTATCAGACTGATGTTG1278330.512125712914904No Hit
TGGCTCAGTTCAGCAGGAAC1241050.49719056582654064No Hit
TGAGGTAGTAGTTTGTACAGT1221660.4894225266086391No Hit
CCCATAAAGTAGAAAGCACT1217560.48777998092563774No Hit
TGGAGTGTGACAATGGTGTTT1144130.458362388363982No Hit
TGAGGGGCAGAGAGCGAGACTTT1126300.45121931774741764No Hit
TCGTACCGTGAGTAATAATGCG1040360.4167899577481164No Hit
TTCACAGTGGCTAAGTTCCG987860.39575736058773325No Hit
TTCAAGTAATCCAGGATAGGCC987170.39548093216791114No Hit
CGCGACCTCAGATC975230.3906975186422926No Hit
TGAGGTAGTAGGTTGTGTGGT870480.34873247954609976No Hit
CGCGACCTCAGATCAGACGT847150.3393859939889238No Hit
TGAGAACTGAATTCCATGGGTT800430.32066898562067436No Hit
TGAGGTAGTAGGTTGTGTGGTT765630.3067273783600776No Hit
AAAAGCTGGGTTGAGAGGGCGA755570.3026971321232499No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT622870.24953473891976743No Hit
TGAGGGGCAGAGAGCGAGACT592020.23717558420742801No Hit
CATTGCACTTGTCTCGGTCTGA587770.23547294538968275No Hit
TGTAAACATCCCCGACTGGAAGCT584760.2342670764858208No Hit
TTCAAGTAATTCAGGATAGGT562470.22533723666286956No Hit
TGAGGTAGTAGGTTGTATAG534850.21427208745201662No Hit
AAACCGTTACCATTACTG529180.21200056695869526No Hit
CGCGACCTCAGATCAGAC523310.2096489222857153No Hit
TGAGGTAGTAGTTTGTACAGTT498980.19990181583024635No Hit
TATTGCACTTGTCCCGGCCTGC475220.19038306328680443No Hit
AAACCGTTACCATTACTGAGTT458880.18383691780869663No Hit
TGAGGTAGTAGTTTGTGCTGT451180.18075213689184047No Hit
CAACGGAATCCCAAAAGCAGCT437990.17546794724335563No Hit
TGTAAACATCCTTGACTGGAAGCT437990.17546794724335563No Hit
AAGCTGCCAGTTGAAGAACTGC436900.17503127046421624No Hit
TCCTGTACTGAGCTGCCCCG431130.1727196878810656No Hit
TACAGTATAGATGATGTACT424400.17002350923555362No Hit
TCCTGTACTGAGCTGCCCCGAGT419080.16789220605663482No Hit
TCCTGTACTGAGCTGCCCCGAGA411010.16465919540741022No Hit
TAGCTTATCAGACTGATGTTGAT409740.16415040686657809No Hit
TTTGGAGCAGGGAGA401160.16071307955922406No Hit
TGAGGTAGTAGGTTGTATAGC393240.15754016204474341No Hit
TGAGGTAGTAGATTGTATAG382780.15334966744859854No Hit
TGTAAACATCCCCGACTGGAAG369430.14800137845638683No Hit
TACAGTACTGTGATAACTGAAG366720.1469156958220128No Hit
TGAGGTAGTAGGTTGTATAGTC357470.14320995251280244No Hit
TGAGGTAGTAGATTGTATAGTC355340.14235662999943832No Hit
CTAGACTGAAGCTCCTTGAGG352680.14129097840997892No Hit
TGAGATGAAGCACTGTAGCT350910.1405818794200003No Hit
AAATGGATTTTTGGAGCAGGGA346300.1387350170788695No Hit
AACCCGTAGATCCGATCTTGT344900.13817414782125928No Hit
TACCCTGTAGAACCGAATTTGT341370.136759956050285No Hit
TTTTTGGAGCAGGGAGA340810.1365356083472409No Hit
TGGAGAGAAAGGCAGTTCCTGA340250.1363112606441968No Hit
AAGCTGCCAGTTGAAGAACT329640.13206067291330797No Hit
TGAGATGAAGCACTGTAGCTC320970.12858728972510758No Hit
TGAGGTAGTAGATTGTATAGC314390.12595120421433956No Hit
TGAGGGGCAGAGAGCGAGACTT307070.12301865923883472No Hit
TCAGTGCATCACAGAACTTTGT306540.12280633016273942No Hit
TGAGGTAGTAGATTGTAT302490.12118381552465272No Hit
AGCTCGGTCTGAGGCCCCTCAGT295420.11835142577372114No Hit
CATTGCACTTGTCTCGGTCTG291340.11671689250868567No Hit
TGAGGTAGTAGGTTGTGTGG284380.1139285710565663No Hit
TGAGGTAGTAGTTTGTGCTGTC282060.11299913057252653No Hit
TGAGGTAGTAGGTTGTAT279340.11190944172916953No Hit
TAGCTTATCAGACTGATGTTGACT274850.11011065389583392No Hit
TTCAAGTAATCCAGGATAGGCA274380.10992236207363622No Hit
ATTTTTGGAGCAGGGAGA274300.10989031240177276No Hit
AAATGGATTTTTGGAGCAGGGAGA273800.10969000195262626No Hit
CAACGGAATCCCAAAAGCAGCTG263980.10575590473138889No Hit
TGTAAACATCCCCGACTGGAAGC261310.10468624693294656No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC258980.10375280023992384No Hit
GGCTGGTCCGATGGTAGT257570.1031879247733307No Hit
GGCTGGTCCGATGGTAGTGGGT253370.10150531700050004No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)