FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11195953
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT5842155.218090858366412No Hit
TCAGTGCACTACAGAACTTTGT2760702.465801705312625No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2073251.8517851941679284No Hit
CGCGACCTCAGATCAGA1339481.1963965908038376No Hit
TCCTGTACTGAGCTGCCCCGAG1337361.1945030494501003No Hit
TTCAAGTAATCCAGGATAGGCT1331441.1892154245377773No Hit
TGAGGTAGTAGGTTGTATAGTT1293661.155471088526363No Hit
TGAGGTAGTAGGTTGTATAGT1179941.053898672136262No Hit
TGAGGTAGTAGATTGTATAGTT1115370.9962260470368177No Hit
TAGCTTATCAGACTGATGTTGAC1096430.9793092200369187No Hit
TCCTGTACTGAGCTGCCCCGA978250.8737532213648985No Hit
AAACCGTTACCATTACTGAG938260.8380349578102017No Hit
TATTGCACTTGTCCCGGCCTGT932060.8324972425304037No Hit
CGCGACCTCAGATC840470.7506908969696461No Hit
AAACCGTTACCATTACTGAGC829720.7410892132183835No Hit
TGAGGTAGTAGATTGTATAGT814960.727905878132929No Hit
TAGCTTATCAGACTGATGTTGA784660.7008425276526259No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT665690.5945809168723735No Hit
TGGCTCAGTTCAGCAGGAAC614660.5490019474001008No Hit
AAAAGCTGGGTTGAGAGGGCGA597300.5334963446166664No Hit
TGAGGGGCAGAGAGCGAGACTTT577480.5157935193189896No Hit
AAGCTGCCAGTTGAAGAACTGT570840.5098628048903028No Hit
TGAGGTAGTAGTTTGTGCTGTT505810.4517793170442927No Hit
TGAGGTAGTAGTTTGTACAGT466260.4164540526384847No Hit
TGAGGTAGTAGGTTGTATAG459610.41051440641095943No Hit
TAGCTTATCAGACTGATGTTG447560.3997515888107069No Hit
TTCACAGTGGCTAAGTTCCG444640.3971435035498988No Hit
TCGTACCGTGAGTAATAATGCG438580.3917308334538382No Hit
CATTGCACTTGTCTCGGTCTGA424600.3792441786777776No Hit
TCAGTGCACTACAGAACTTTGC422560.3774220917147473No Hit
TGGAGTGTGACAATGGTGTTT397970.35545879837116146No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT389420.34782211036434324No Hit
TGAGGTAGTAGGTTGTGTGGTT384760.3436598921056564No Hit
TGAGGTAGTAGGTTGTGTGGT383270.3423290540787372No Hit
CCCATAAAGTAGAAAGCACT364130.3252335911020705No Hit
TGAGAACTGAATTCCATGGGTT337430.30138568820358574No Hit
TCCTGTACTGAGCTGCCCCGAGA328620.2935167734269696No Hit
TCCTGTACTGAGCTGCCCCG308340.2754030853827271No Hit
GGCTGGTCCGATGGTAGTG303500.2710800947449494No Hit
CGCGACCTCAGATCAGACGT296820.26511365312090895No Hit
TGTAAACATCCCCGACTGGAAG291660.2605048449203029No Hit
TTTGGAGCAGGGAGA290040.25905789350848474No Hit
GGCTGGTCCGATGGTAGT281700.2516087732772726No Hit
CGCGACCTCAGATCAGAC275470.24604426260095946No Hit
TGGAGAGAAAGGCAGTTCCTGA273320.24412392585070694No Hit
ATTTTTGGAGCAGGGAGA271770.24273949703075745No Hit
TGAGGTAGTAGTTTGTACAGTT271680.242659110841212No Hit
AAATGGATTTTTGGAGCAGGGAGA271620.24260552004818167No Hit
TTTTTGGAGCAGGGAGA270580.24167661296898976No Hit
TGAGGTAGTAGATTGTATAG266430.23796991645106047No Hit
AAATGGATTTTTGGAGCAGGGA259370.23166406647116153No Hit
TGTAAACATCCCCGACTGGAAGCT251630.2247508541702524No Hit
GGCTGGTCCGATGGTAGTGGGT242200.21632816786565645No Hit
TTCAAGTAATCCAGGATAGGCC235160.21004018148343423No Hit
TGAGGGGCAGAGAGCGAGACT227940.20359142272212113No Hit
AAACCGTTACCATTACTG219730.196258415875808No Hit
TGAGATGAAGCACTGTAGCTC203130.18143162980409083No Hit
AAATGGATTTTTGGAGCAGGGAG202670.18102076705752518No Hit
TCCTGTACTGAGCTGCCCCGAGT199490.1781804550269191No Hit
TGTAAACATCCCCGACTGGAAGC189100.1689003160338383No Hit
CTAGACTGAAGCTCCTTGAGG175140.15643152485545447No Hit
GGCTGGTCCGATGGTAGTGGGTTATC173700.1551453458227272No Hit
TGAGGTAGTAGGTTGTATAGC172830.1543682793237878No Hit
TGAGGTAGTAGTTTGTGCTGT172670.15422537054237365No Hit
TGAGATGAAGCACTGTAGCT172540.15410925715747467No Hit
TGAGGTAGTAGGTTGTGTGG172410.15399314377257567No Hit
CAACGGAATCCCAAAAGCAGCTG170680.15244794257353528No Hit
GGCTGGTCCGATGGTAGTGGGTT168510.15050974222560598No Hit
TGAGGTAGTAGGTTGTATAGTC167920.14998276609414135No Hit
AGAGGTAGTAGGTTGCATAGTT167810.14988451630691912No Hit
TGAGGGGCAGAGAGCGAGACTT166060.14832145151020196No Hit
CAACGGAATCCCAAAAGCAGCT165720.14801777034969688No Hit
CATTGCACTTGTCTCGGTCTG162230.14490057255510094No Hit
TTCAAGTAATTCAGGATAGGT161620.14435573282595954No Hit
TGTAAACATCCTTGACTGGAAGCT159960.14287305421878782No Hit
TGGATTTTTGGAGCAGGGAGA157220.14042574133707061No Hit
TACAGTACTGTGATAACTGAAG157190.1403989459405555No Hit
TATTGCACTTGTCCCGGCCTGC155400.1388001539484848No Hit
AAAAGCTGGGTTGAGAGGGCG155190.13861258617287872No Hit
TGGCTCAGTTCAGCAGGAACAG154470.13796949665651506No Hit
AAATGGATTTTTGGAGCAGGG153180.13681729460636355No Hit
AAACCGTTACCATTACTGAGTT152990.13664759042843427No Hit
AGCTCGGTCTGAGGCCCCTCAGT147390.13164578307893932No Hit
TGAGGTAGTAGATTGTATAGTC143580.1282427677215151No Hit
TGAGGTAGTAGGTTGTAT143570.1282338359226767No Hit
TATTGCACTTGTCCCGGCCTG138670.12385725449186862No Hit
GGCTGGTCCGATGGTAGTGG137960.12322309677434337No Hit
AAAAGCTGGGTTGAGAGGGCGAA135550.12107053325429286No Hit
AGAGGTAGTAGGTTGCATAGT133940.11963251364131305No Hit
TTTTGGAGCAGGGAGA133130.11890903793540397No Hit
AGCAGCATTGTACAGGGCTATGA133080.11886437894121206No Hit
CGCGACCTCAGATCAGACG133030.11881971994702015No Hit
TGTAAACATCCCCGACTGGAA132680.11850710698767672No Hit
AAAAGCTGGGTTGAGAGGGCGAT131830.11774790408641408No Hit
TACCCTGTAGAACCGAATTTGT131550.11749781371893935No Hit
GGCTGGTCCGATGGTAGTGGG127700.11405907116616157No Hit
TGAAAAGAACTTTGAAGAGAG124430.11113837294601005No Hit
TGAGGTAGTAGATTGTAT121110.10817301573166661No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA120170.10733342664085853No Hit
TGAGGTAGTAGATTGTATAGC118490.10583288443601004No Hit
AAATGGATTTTTGGAGCAGGGAGAT118450.10579715724065651No Hit
CGTGTATTTGACAAGCTGAGTTG116770.10429661503580803No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC114330.10211725611924238No Hit
AACATTCAACGCTGTCGGTGA112340.10033982815040399No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)