FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11470903
Sequences flagged as poor quality0
Sequence length14-50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT152768113.317879159121127No Hit
AAACCGTTACCATTACTGAGC2536222.2110029175558368No Hit
TTCAAGTAATCCAGGATAGGCT2462192.146465714163915No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC2390212.083715641218481No Hit
TGGAGTGTGACAATGGTGTTT2148001.8725639995386587No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1832461.5974853941315692No Hit
TCAGTGCACTACAGAACTTTGT1740911.5176747637043047No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1588391.3847122584856657No Hit
TGAGGTAGTAGGTTGTATAGT1468371.2800823091259685No Hit
TATTGCACTTGTCCCGGCCTGT1409511.2287698710380517No Hit
TGAGGTAGTAGATTGTATAGT1212291.0568392043764994No Hit
TGAGGTAGTAGATTGTATAGTT1156661.0083425864554865No Hit
TGAGGTAGTAGGTTGTATAGTT1113590.9707954116602677No Hit
TAGCTTATCAGACTGATGTTGAC1105920.9641089284775575No Hit
TCCTGTACTGAGCTGCCCCGAG1048700.9142261947468302No Hit
TGAGGTAGTAGTTTGTGCTGTT1027140.8954308130754831No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1026690.8950385161482055No Hit
TGAGGTAGTAGTTTGTACAGT839810.7321219611045443No Hit
AAACCGTTACCATTACTGAG827460.7213555898781465No Hit
TCCTGTACTGAGCTGCCCCGA777900.6781506216206344No Hit
AAGCTGCCAGTTGAAGAACTGT690630.6020711708572551No Hit
TGAGGTAGTAGGTTGTGTGGT683500.5958554439872781No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC680460.5932052603007801No Hit
TAGCTTATCAGACTGATGTTGA671160.5850977904703754No Hit
TGAGGTAGTAGGTTGTGTGGTT551630.48089500887593595No Hit
TCGTACCGTGAGTAATAATGCG542280.47274395049805584No Hit
TTCAAGTAATCCAGGATAGGCC521310.45446291368691727No Hit
CATTGCACTTGTCTCGGTCTGA463250.403847892358605No Hit
CCCATAAAGTAGAAAGCACT457420.39876546772298577No Hit
TTCAAGTAATTCAGGATAGGT426730.37201081728264984No Hit
CGCGACCTCAGATCAGA420050.3661873873399505No Hit
TAGCTTATCAGACTGATGTTG413870.36079984287200406No Hit
TGAGGGGCAGAGAGCGAGACTTT375660.3274894748913839No Hit
TACAGTATAGATGATGTACT374220.3262341247240954No Hit
TGAGGTAGTAGTTTGTGCTGT373210.32535363606509443No Hit
TGAGGTAGTAGGTTGTATAG356860.3111001810406731No Hit
TCCTGTACTGAGCTGCCCCGAGT354410.3089643422143836No Hit
TGTAAACATCCCCGACTGGAAGCT333950.29112790858749305No Hit
TGGAGTGTGACAATGGTGTTC321710.280457432165541No Hit
TCCTGTACTGAGCTGCCCCGAGA319400.27844364127218235No Hit
TGAGGTAGTAGTTTGTACAGTT312820.27270738842443354No Hit
TCAGTGCACTACAGAACTTTGC311670.27170485183250176No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT305930.2667008865823379No Hit
TATTGCACTTGTCCCGGCCTGC305640.2664480730069812No Hit
AAACCGTTACCATTACTG293580.25593451535594014No Hit
TGAGGGGCAGAGAGCGAGACT292190.25472275373612696No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC287990.25106131574820223No Hit
TGAGAACTGAATTCCATGGGTT279090.24330255429759975No Hit
TCCTGTACTGAGCTGCCCCG270270.23561353452295777No Hit
AAAAGCTGGGTTGAGAGGGCGA263500.2297116451948029No Hit
TGAGGTAGTAGATTGTATAG255260.22252825257087433No Hit
CGCGACCTCAGATCAGACGT252740.22033138977811945No Hit
TGGAGTGTGACAATGGTGTTTG250000.21794273737647335No Hit
TGAGGTAGTAGGTTGTATAGC249250.21728890916434393No Hit
TGGCTCAGTTCAGCAGGAAC248180.2163561142483726No Hit
CGCGACCTCAGATC239770.20902452056302803No Hit
AAACCGTTACCATTACTGAGTT239700.20896349659656263No Hit
TACAGTACTGTGATAACTGAAG238140.20760353391533343No Hit
TGTAAACATCCTTGACTGGAAGCT229730.20027194022998887No Hit
TTCACAGTGGCTAAGTTCCG222260.19375981123717986No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG215800.18812817090337178No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT207300.1807181178325717No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC205810.17941917911780791No Hit
CATTGCACTTGTCTCGGTCTG204650.17840792481638107No Hit
TGAGGTAGTAGATTGTATAGC199380.17381369191248502No Hit
TGAGGTAGTAGATTGTATAGTC194160.16926304755606425No Hit
TGAGGTAGTAGGTTGTATAGTC191880.1672754097911908No Hit
TAGCTTATCAGACTGATGTTGAT191460.16690926599239833No Hit
TGAGGTAGTAGATTGTAT184690.16100737666424342No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC181870.15854898258663683No Hit
CTAGACTGAAGCTCCTTGAGG179930.15685774694459537No Hit
TGAGGTAGTAGTTTGTGCTGTC177520.15475677895628617No Hit
TGAGGTAGTAGGTTGTAT177080.15437319973850358No Hit
AAACCGTTACCATTACTGAGA174230.1518886525324118No Hit
AACCCGTAGATCCGATCTTGT169810.14803542493559574No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT165140.14396425460140322No Hit
TGAGGTAGTAGGTTGTGTGG162340.14152329594278673No Hit
TGTAAACATCCCCGACTGGAAG157820.13758289125102008No Hit
TTCAAGTAATCCAGGATAGGCA155290.13537731074877016No Hit
TGGAGAGAAAGGCAGTTCCTGA153780.13406093661501628No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA151720.13226508845903415No Hit
AGAGGTAGTAGGTTGCATAGT151090.1317158727608454No Hit
ACCAATATTACTGTGCTGCTT150390.1311056330961913No Hit
TACAGTACTGTGATAACTGA148420.12938824432566468No Hit
GCATTGGTGGTTCAGTGGTAGAATT145170.12655498873977053No Hit
TGAGGTAGTAGTTTGTACAGC145100.1264939647733051No Hit
TCAGTGCATCACAGAACTTTGT144100.12562219382379922No Hit
TATTGCACTTGTCCCGGCCTG142410.12414890091913428No Hit
CAACGGAATCCCAAAAGCAGCT141380.12325097684114321No Hit
AGCTCGGTCTGAGGCCCCTCAGT139100.12126333907626977No Hit
TGAGGTAGTAGTTTGTGCTG137370.11975517533362456No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT135460.11809009282006831No Hit
AGAGGTAGTAGGTTGCATAGTT134540.1172880635465229No Hit
TGAGGTAGTAGGTTGTGTGGC132270.11530914349114452No Hit
AAACCGTTACCATTACTGAGG129440.11284203170404282No Hit
AAGCTGCCAGTTGAAGAACTGC129060.11251075874323059No Hit
TACAGTACTGTGATAACTGAA127630.11126412628543716No Hit
CAACGGAATCCCAAAAGCAGCTG127200.11088926477714964No Hit
TAGCTTATCAGACTGATGTTGACT123910.10802113835327525No Hit
TGTAAACATCCCCGACTGGAAGC121510.10592888807446109No Hit
TTCAAGTAATTCAGGATAGGTT119780.10442072433181589No Hit
AGACCGTTACCATTACTGAGT118530.10333101064493354No Hit
TGAGATGAAGCACTGTAGCTC115810.1009597936622775No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)