FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12875512
Sequences flagged as poor quality0
Sequence length14-50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT144151411.19578002024308No Hit
TCAGTGCACTACAGAACTTTGT3061232.377559820533739No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2122561.6485247343950282No Hit
CGCGACCTCAGATCAGA2096051.6279352619142446No Hit
TTCAAGTAATCCAGGATAGGCT2027431.5746402939160786No Hit
TCCTGTACTGAGCTGCCCCGAG1887891.4662640211899922No Hit
TAGCTTATCAGACTGATGTTGAC1707021.3257880540983535No Hit
AAACCGTTACCATTACTGAG1626311.263103168246824No Hit
AAACCGTTACCATTACTGAGC1574081.22253779111852No Hit
TGGAGTGTGACAATGGTGTTT1491201.158167535395874No Hit
TGAGGTAGTAGGTTGTATAGT1337101.0384829745022954No Hit
TAGCTTATCAGACTGATGTTGA1305871.0142276283847975No Hit
TCCTGTACTGAGCTGCCCCGA1301601.0109112554126003No Hit
TGAGGTAGTAGATTGTATAGT1083420.8414577998917636No Hit
TGAGGTAGTAGGTTGTATAGTT1073830.834009552396829No Hit
TGAGGTAGTAGATTGTATAGTT1051750.8168607197911818No Hit
TATTGCACTTGTCCCGGCCTGT1040720.808294070169792No Hit
TGAGGTAGTAGTTTGTGCTGTT875180.6797244257160414No Hit
TCGTACCGTGAGTAATAATGCG856940.6655579987809417No Hit
TTCACAGTGGCTAAGTTCCG836670.6498149355147974No Hit
TAGCTTATCAGACTGATGTTG802720.6234470520473283No Hit
CATTGCACTTGTCTCGGTCTGA750870.5831768088135059No Hit
AAGCTGCCAGTTGAAGAACTGT748960.5816933726596658No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT737410.5727228556037227No Hit
TGAGGTAGTAGTTTGTACAGT711920.5529255846291783No Hit
TGGCTCAGTTCAGCAGGAAC696960.5413066292043377No Hit
TGAGGTAGTAGGTTGTATAG643570.4998403170297228No Hit
AAAAGCTGGGTTGAGAGGGCGA591670.4595312403887318No Hit
CCCATAAAGTAGAAAGCACT574400.4461181815527025No Hit
CGCGACCTCAGATC566480.4399669698571987No Hit
AAACCGTTACCATTACTG523610.40667120655085404No Hit
TGAGGTAGTAGGTTGTGTGGT520970.4046208026523528No Hit
TACAGTACTGTGATAACTGAAG499070.38761177031251265No Hit
TGAGGTAGTAGATTGTATAG469430.36459132654297555No Hit
TCCTGTACTGAGCTGCCCCG459330.3567469782949214No Hit
TCCTGTACTGAGCTGCCCCGAGA457940.35566740957563475No Hit
TGAGGTAGTAGGTTGTGTGGTT438630.3406699477271273No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC409810.318286371835155No Hit
CGCGACCTCAGATCAGACGT385680.29954536953559596No Hit
TGAGGTAGTAGTTTGTGCTGT377010.2928116567325633No Hit
TTCAAGTAATTCAGGATAGGT373960.2904428188952797No Hit
TGAGGGGCAGAGAGCGAGACTTT373060.2897438175662451No Hit
TCAGTGCACTACAGAACTTTGC361770.2809752342275787No Hit
TGTAAACATCCCCGACTGGAAG354270.275150223152291No Hit
CATTGCACTTGTCTCGGTCTG351780.27321631947529545No Hit
TGGAGAGAAAGGCAGTTCCTGA324890.2523317131000305No Hit
TGAGAACTGAATTCCATGGGTT316830.24607176786445462No Hit
TGAGGTAGTAGTTTGTACAGTT312050.24235929413913793No Hit
TGAGATGAAGCACTGTAGCTC299260.2324257085854139No Hit
CTAGACTGAAGCTCCTTGAGG294650.2288452684444704No Hit
TACAGTATAGATGATGTACT286330.2223833894916179No Hit
TTCAAGTAATCCAGGATAGGCC277240.21532347606836919No Hit
TCCTGTACTGAGCTGCCCCGAGT268800.20876839693831206No Hit
TGAGGTAGTAGGTTGTGTGG266630.20708302706719545No Hit
TGGAGTGTGACAATGGTGTTTG264350.205312223700308No Hit
TACAGTACTGTGATAACTGA256670.19934741235921338No Hit
AAACCGTTACCATTACTGAGTT255720.19860957762301026No Hit
TGTAAACATCCCCGACTGGAAGCT245350.19055552897624578No Hit
TGAGGGGCAGAGAGCGAGACT243360.1890099593709361No Hit
TGAGGTAGTAGTTTGTGCTG229750.1784395059396473No Hit
TGTAAACATCCTTGACTGGAAGCT223540.17361639676930907No Hit
TGGCTCAGTTCAGCAGGAACAG221500.17203199375683081No Hit
AAAAGCTGGGTTGAGAGGGCG218490.169694222645282No Hit
TGTAAACATCCCCGACTGGAAGC217340.16880105428040454No Hit
TACAGTACTGTGATAACTGAA217080.1685991205631279No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG216910.1684670869787547No Hit
TGAGATGAAGCACTGTAGCT207610.16124407324539794No Hit
TAGCAGCACGTAAATATTGGCG204310.15868106837227133No Hit
TGGAGAGAAAGGCAGTTCCTG195890.15214152260508165No Hit
TATTGCACTTGTCCCGGCCTG190610.1480407148080791No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT185350.14395544037394398No Hit
CGCGACCTCAGATCAGAC183620.14261180448591093No Hit
CAACGGAATCCCAAAAGCAGCTG175210.13608002540015496No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC172010.13359468734136554No Hit
TAGCAGCACGTAAATATTGGC165730.12871721140099127No Hit
AACATTCAACGCTGTCGGTGA160450.1246164036039887No Hit
ACCAATATTACTGTGCTGCTT157800.12255823302405372No Hit
TGAGGTAGTAGATTGTAT157460.12229416585530734No Hit
TGAGGTAGTAGGTTGTAT156700.12170389806634486No Hit
TCGTACCGTGAGTAATAATGC155080.1204456956740827No Hit
TCAGTGCATCACAGAACTTTGT154120.11970009425644587No Hit
GTACAGTACTGTGATAACTGA153420.11915642655608569No Hit
TGAGGTAGTAGGTTGTATAGC152060.11810015788110018No Hit
AGGAAACTCTGGTG151750.11785939075665496No Hit
AGAGGTAGTAGGTTGCATAGT149770.116321587832779No Hit
TAAAGTGCTTATAGTGCAGGTAG149060.1157701534509851No Hit
TGTAAACATCCCCGACTGGAA147090.11424011720854285No Hit
GGCTGGTCCGATGGTAGTG143060.11111014459075491No Hit
AGCAGCATTGTACAGGGCTATG141250.10970437525125215No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC139750.10853937303619461No Hit
TTCACAGTGGCTAAGTTCCGA135050.10488903276234764No Hit
CAACGGAATCCCAAAAGCAGCT134960.10481913262944417No Hit
AGAGGTAGTAGGTTGCATAGTT133540.10371626386585636No Hit
AAAAGCTGGGTTGAGAGGGCGAA130970.1017202267373911No Hit
AAATGGATTTTTGGAAATAGGAGA130290.10119209239989836No Hit
AGCTCGGTCTGAGGCCCCTCAGT129890.10088142514254968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)