FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14270221
Sequences flagged as poor quality0
Sequence length14-50
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTGGTGGTTCAGTGGTAGAATTCTCGC8573726.008119986368817No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6463544.529390259618263No Hit
AAACCGTTACCATTACTGAGT5840444.092746706585693No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3685722.5828051296472565No Hit
TCCTGTACTGAGCTGCCCCGAG2343311.6420979044403026No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC2256831.5814961800521519No Hit
TCCTGTACTGAGCTGCCCCGA2015941.412690104799358No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC1496621.0487714240725494No Hit
CGCGACCTCAGATCAGA1465831.0271950238191825No Hit
TATTGCACTTGTCCCGGCCTGT1308890.9172177501665882No Hit
TCAGTGCACTACAGAACTTTGT1283600.8994955298870283No Hit
TAGCTTATCAGACTGATGTTGAC1054720.7391055821770385No Hit
TTCAAGTAATCCAGGATAGGCT973280.6820356881648855No Hit
TAGCTTATCAGACTGATGTTGA791560.5546935818303025No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG785890.550720272657305No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT779240.5460602186889748No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC743530.5210360792590388No Hit
TGAGGGGCAGAGAGCGAGACTTT683420.4789133959453046No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC652170.4570146460941285No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT645320.45221444012675066No Hit
AAACCGTTACCATTACTGAGC641160.4492992785465621No Hit
TCCTGTACTGAGCTGCCCCG586440.4109536916071587No Hit
TCCTGTACTGAGCTGCCCCGAGA568750.39855724729140496No Hit
AAACCGTTACCATTACTGAG548760.38454905498660463No Hit
CGCGACCTCAGATC517670.3627624267346666No Hit
TGAGGTAGTAGGTTGTATAGTT484230.33932901249392No Hit
TAGCTTATCAGACTGATGTTG466640.32700264417769004No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG431260.3022097555461825No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC421210.2951671175940443No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG418760.2934502556057121No Hit
TGAGGTAGTAGATTGTATAGTT407430.28551064485966965No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC403890.2830299544765284No Hit
AAAAGCTGGGTTGAGAGGGCGA397000.27820171810934113No Hit
CATTGCACTTGTCTCGGTCTGA395690.2772837225155798No Hit
TTCACAGTGGCTAAGTTCCG391050.2740321961376772No Hit
TGAGGGGCAGAGAGCGAGACT385870.27040225936234624No Hit
TCGTACCGTGAGTAATAATGCG381970.2676692953809195No Hit
AAGCTGCCAGTTGAAGAACTGT381950.26765528018101475No Hit
TGGCTCAGTTCAGCAGGAAC378820.2654619013959209No Hit
TGTAAACATCCCCGACTGGAAG365150.25588251226102243No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC357840.25075995669583534No Hit
GCATTGGTGGTTCAGTGGTAGAATT353170.24748740751807558No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA345100.24183227435650786No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA341970.23963889557141407No Hit
TCCTGTACTGAGCTGCCCCGAGT341840.2395477967720332No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT335710.23525213800122646No Hit
CGCGACCTCAGATCAGACGT314590.2204520869018076No Hit
TGAGGTAGTAGGTTGTATAGT302200.2117696705608133No Hit
CCCATAAAGTAGAAAGCACT286490.20076073103563008No Hit
TGGAGTGTGACAATGGTGTTT284940.1996745530430117No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT283100.19838515465177448No Hit
TACAGTACTGTGATAACTGAAG272940.19126543310016011No Hit
AGCGCACGTGTTAGGAC262130.18369021755164128No Hit
TGTAAACATCCCCGACTGGAAGCT260210.182344758360785No Hit
TGAGGTAGTAGTTTGTGCTGTT248540.1741668892163618No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGC240390.1684556952551751No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCC237210.16622727847031943No Hit
AAACCGTTACCATTACTG236020.16539337407598662No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG233850.16387272488632096No Hit
TATTGCACTTGTCCCGGCCTG226960.15904448851913366No Hit
GTTCTATTTTGTTGGTTTTCGGAAC224880.15758690772903938No Hit
CGCGACCTCAGATCAGAC220180.15429333575142248No Hit
TGAGGTAGTAGGTTGTGTGGTT217170.1521840481657572No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA217110.15214200256604293No Hit
AGGGCGTGAAACCGTTAAGAGG213160.14937400058485428No Hit
TGAGGTAGTAGGTTGTGTGG208940.14641679340495148No Hit
TGTAAACATCCCCGACTGGAAGC205070.14370485222338183No Hit
TGAGGTAGTAGGTTGTGTGGT204710.14345257862509628No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC204010.14296204662842993No Hit
TGAGGTAGTAGATTGTATAGT201170.14097188824195506No Hit
TGAGAACTGAATTCCATGGGTT197630.1384911978588138No Hit
TGAGGGGCAGAGAGCGAGACTT197330.13828096986024252No Hit
GCATTGTGGTTCAGTGGTAGAATTCTC186620.13077583031124745No Hit
CAACGGAATCCCAAAAGCAGCTG185670.13011010831577172No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC177310.12425175475558507No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT175890.12325667556234764No Hit
GGTGGGCGTAGCTC175690.12311652356330009No Hit
GTCTGAGCGTCGCT170950.11979492118587372No Hit
TGAGGTAGTAGTTTGTACAGT170430.11943052598835016No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG168770.11826726439625568No Hit
CATTGCACTTGTCTCGGTCTG168630.1181691579969224No Hit
GTGAAATGTTTAGGAC163550.11460929722111521No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGA163240.11439206162259155No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT160370.11238088043625954No Hit
AGAAGCCGGCGGGAGC160270.11231080443673577No Hit
CGCGACCTCAGATCAGACG156040.10934658965688059No Hit
GGCTGGTCCGATGGTAGTGGGTTATC155950.10928352125730918No Hit
TATTGCACTTGTCCCGGCCTGC154130.1080081380659767No Hit
AGTTGGTCCGAGTGTTGTGGGT151820.10638938247697777No Hit
CTAGACTGAAGCTCCTTGAGG151460.1061371088786922No Hit
GTTTCCGTAGTGTAGTGGTTATC150510.10547138688321644No Hit
AACATTCAACGCTGTCGGTGA148690.10419600369188395No Hit
TCAGTGCACTACAGAACTTTGC148540.1040908896925983No Hit
GCGCACGTGTTAGGAC144600.10132989531136202No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGC144170.10102856851340984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)