FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9380803
Sequences flagged as poor quality0
Sequence length14-50
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT4564514.865798802085493No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2812942.9986132317244056No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1475621.5730209876489252No Hit
TCAGTGCACTACAGAACTTTGT1420501.5142626915840787No Hit
TCCTGTACTGAGCTGCCCCGAG1030921.0989677536134166No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC962811.0263620289222575No Hit
CGCGACCTCAGATCAGA961271.0247203784153658No Hit
TCCTGTACTGAGCTGCCCCGA826830.8814064211773769No Hit
TAGCTTATCAGACTGATGTTGAC728280.7763514488045427No Hit
TTCAAGTAATCCAGGATAGGCT728250.776319468599863No Hit
TATTGCACTTGTCCCGGCCTGT669780.713990049679116No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT594160.6333786137498038No Hit
TGAGGTAGTAGGTTGTATAGT590920.6299247516443954No Hit
TGGAGTGTGACAATGGTGTTT574160.6120584772966664No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC538120.5736395914081129No Hit
AAACCGTTACCATTACTGAGC484440.5164163451678924No Hit
TGAGGGGCAGAGAGCGAGACTTT480300.5120030769220929No Hit
TGAGGTAGTAGGTTGTATAGTT436510.4653226381579488No Hit
TAGCTTATCAGACTGATGTTGA411590.4387577481373396No Hit
TGAGGTAGTAGGTTGTGTGGT399150.42549662326348825No Hit
TCCTGTACTGAGCTGCCCCGAGA398130.42440929630437824No Hit
TGAGGTAGTAGGTTGTGTGGTT361020.3848497831155819No Hit
TGAGGTAGTAGATTGTATAGT353360.37668417085403033No Hit
AAGCTGCCAGTTGAAGAACTGT332890.35486301119424424No Hit
TGAGGTAGTAGTTTGTGCTGTT328940.3506522842447496No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA317810.3387876283085787No Hit
TGAGGTAGTAGATTGTATAGTT307730.32804227953619747No Hit
AAACCGTTACCATTACTGAG299750.3195355450913957No Hit
CATTGCACTTGTCTCGGTCTGA286150.3050378523032623No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC284560.3033429014552379No Hit
CCCATAAAGTAGAAAGCACT278980.2973945833848126No Hit
TGAGGGGCAGAGAGCGAGACT274600.2927254735015755No Hit
TTCACAGTGGCTAAGTTCCG268760.2864999936572594No Hit
TCGTACCGTGAGTAATAATGCG268460.2861801916104623No Hit
TCCTGTACTGAGCTGCCCCG244810.2609691302546274No Hit
TGGCTCAGTTCAGCAGGAAC241660.2576112087632583No Hit
TGAGGTAGTAGGTTGTATAG239330.25512741286646784No Hit
TGAGGTAGTAGTTTGTACAGT223910.23868958766109896No Hit
TAGCTTATCAGACTGATGTTG217120.23145140133525882No Hit
CGCGACCTCAGATCAGACGT205410.21896846144194693No Hit
TCCTGTACTGAGCTGCCCCGAGT184910.19711532157748116No Hit
AAACCGTTACCATTACTG184390.1965609980296996No Hit
AAAAGCTGGGTTGAGAGGGCGA180820.19275535367281457No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA180810.192744693604588No Hit
TGTAAACATCCCCGACTGGAAGCT180010.19189188814646252No Hit
TGAGGTAGTAGGTTGTGTGG176670.18833142535878858No Hit
CTAGACTGAAGCTCCTTGAGG176240.18787304242504613No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT173140.18456842127480985No Hit
TGTAAACATCCCCGACTGGAAG172770.1841739987504268No Hit
CGCGACCTCAGATC165800.17674393119650844No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC165040.1759337660112892No Hit
TGTAAACATCCCCGACTGGAAGC162410.17313016806770168No Hit
GGCCTTGAGGGCTGTTGTGGAGAAG160120.17068901244381743No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC159820.17036921039702038No Hit
TCAGTGCACTACAGAACTTTGC159740.17028392985120783No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG154580.1647833346462984No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT139420.1486226712148203No Hit
TATTGCACTTGTCCCGGCCTG135230.14415610262788803No Hit
TGGCTCAGTTCAGCAGGAACAG133540.14235455109759795No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGC130190.13878342824169743No Hit
CATTGCACTTGTCTCGGTCTG127290.13569200845599252No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT127170.1355640876372737No Hit
TGAGGTAGTAGATTGTATAG125450.13373055590230387No Hit
TGAGATGAAGCACTGTAGCTC121990.13004217229591114No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT116310.12398725354322013No Hit
TTCAAGTAATTCAGGATAGGT114080.12161005832869531No Hit
TACAGTACTGTGATAACTGAAG112160.11956332522919412No Hit
TGAGGGGCAGAGAGCGAGACTT110290.11756989247082579No Hit
TGAGATGAAGCACTGTAGCT107520.11461705357206627No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA103950.11081140921518126No Hit
AGAAGCCGGCGGGAGC103680.1105235873730639No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG103240.1100545443710949No Hit
AGGAGTGGAGCCTGCGGCTTA101430.10812507202208595No Hit
CGGACACGCTACCTGCGGGACTGTGC100290.10690982424425713No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA99600.10617427953662388No Hit
AGCTCGGTCTGAGGCCCCTCAGT97980.10444734848391976No Hit
TTCAAGTAATCCAGGATAGGCC97860.10431942766520094No Hit
TGAGAACTGAATTCCATGGGTT96910.10330672118367693No Hit
AAATGGATTTTTGGAGCAGGGAGA96150.1024965559984577No Hit
CGGACACGCTACCTGCGGGACTGTG95450.1017503512225979No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG94570.10081226521865985No Hit
CAACGGAATCCCAAAAGCAGCTG94130.10034322221669084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)