FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13035833
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7537695.7822848758495144No Hit
TGGAGTGTGACAATGGTGTTT3250512.493519209704512No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC3216752.4676213633605157No Hit
TCAGTGCACTACAGAACTTTGT2603451.9971489355532552No Hit
TTCAAGTAATCCAGGATAGGCT2594651.9903983120986593No Hit
TATTGCACTTGTCCCGGCCTGT2194331.683306314218662No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2151571.650504421159737No Hit
TAGCTTATCAGACTGATGTTGAC2137601.6397878064255658No Hit
TCCTGTACTGAGCTGCCCCGAG1479731.1351250050533785No Hit
AAACCGTTACCATTACTGAGC1399651.0736943316165526No Hit
TGAGGGGCAGAGAGCGAGACTTT1370831.05158603980275No Hit
TCCTGTACTGAGCTGCCCCGA1220520.9362807885004357No Hit
TGAGGTAGTAGGTTGTATAGTT1161860.8912817462451382No Hit
AAGCTGCCAGTTGAAGAACTGT1013580.7775337410351912No Hit
TGAGGTAGTAGATTGTATAGTT939210.7204833016808362No Hit
TGAGGTAGTAGTTTGTGCTGTT846240.64916449911563No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC802970.6159713767428594No Hit
CGCGACCTCAGATCAGA796630.6111078593903435No Hit
TGAGGTAGTAGGTTGTATAGT761640.5842664599953068No Hit
TGAGGTAGTAGGTTGTGTGGTT718750.5513648418171666No Hit
TCGTACCGTGAGTAATAATGCG717140.5501297845714961No Hit
TAGCTTATCAGACTGATGTTGA705300.5410471275598575No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT626050.48025316065340823No Hit
TGAGGGGCAGAGAGCGAGACT619230.47502142747609605No Hit
TTCAAGTAATCCAGGATAGGCC597170.45809884186150585No Hit
AAACCGTTACCATTACTGAGG580950.44565621544860234No Hit
TGAGGTAGTAGGTTGTGTGGT573980.4403094148260414No Hit
TGGAGTGTGACAATGGTGTTC545710.4186230369781509No Hit
TATTGCACTTGTCCCGGCCTGC513120.393622716707095No Hit
TCAGTGCACTACAGAACTTTGC504780.3872249667512617No Hit
TCCTGTACTGAGCTGCCCCG492660.37792751717515866No Hit
TGTAAACATCCCCGACTGGAAGCT492410.377735738099744No Hit
TAGCTTATCAGACTGATGTTG481000.3689829410978186No Hit
TGAGGTAGTAGATTGTATAGT464070.3559956621107374No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC436360.3347388693917757No Hit
TGAGAACTGAATTCCATGGGTT414800.31819984192801487No Hit
TCCTGTACTGAGCTGCCCCGAGT412480.3164201321081668No Hit
TCCTGTACTGAGCTGCCCCGAGA398480.30568050388494544No Hit
TGGCTCAGTTCAGCAGGAAC385520.2957386766154491No Hit
TGGAGTGTGACAATGGTGTTTG384690.29510197008507244No Hit
CCCATAAAGTAGAAAGCACT368180.28243687994468786No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT349040.267754273930941No Hit
TTCAAGTAATCCAGGATAGGCG342940.2630748644908231No Hit
TAGCTTATCAGACTGATGTTGAT330440.2534859107200898No Hit
CTAGACTGAAGCTCCTTGAGG323590.24823116405372792No Hit
TTCACAGTGGCTAAGTTCCG316350.2426772420297192No Hit
TGAGGTAGTAGTTTGTACAGT305850.23462252086230315No Hit
TGAGGTAGTAGGTTGTGTGG299580.22981270165090334No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC297280.2280483341570884No Hit
TATTGCACTTGTCCCGGCCTG296750.2276417625172093No Hit
AAAAGCTGGGTTGAGAGGGCGA294980.22628396666327347No Hit
GTGAAATGTTTAGGAC276930.21243751741833453No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG274030.2102128801435244No Hit
TAGCTTATCAGACTGATGTTGACT258260.19811545606636724No Hit
AGCTCGGTCTGAGGCCCCTCAGT256710.19692642579879627No Hit
CATTGCACTTGTCTCGGTCTGA254260.19504699085973254No Hit
TGGCTCAGTTCAGCAGGAACAG254050.19488589643638424No Hit
AAACCGTTACCATTACTGAG245690.18847280415451778No Hit
TGAGGGGCAGAGAGCGAGACTT241190.18502078079705378No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT239600.1838010658774165No Hit
CGCGACCTCAGATCAGACGT229170.1758000428511166No Hit
TGAGGTAGTAGGTTGTATAGTC227170.17426581024779927No Hit
TGTAAACATCCCCGACTGGAAGC226810.17398964837920217No Hit
TCAGTGCACTACAGAACTTTGG215020.16494534718264647No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT214230.16433932530433615No Hit
TTCAAGTAATCCAGGATAGGCA212550.16305056991754957No Hit
TGTAAACATCCTTGACTGGAAGCT212080.16269002525577No Hit
AACCCGTAGATCCGATCTTGT212070.16268235409275342No Hit
AAGCTGCCAGTTGAAGAACTGC206510.1584171874555312No Hit
TGAGGGGCAGAGAGCGAGACTTC205490.15763472882783938No Hit
TATTGCACTTGTCCCGGCCTGG205210.15741993626337494No Hit
AAACCGTTACCATTACTG199350.15292463473565518No Hit
CGCGACCTCAGATC195700.15012466023460105No Hit
TACCCTGTAGAACCGAATTTGT190500.14613565546597598No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACG188500.14460142286265865No Hit
TGAGGTAGTAGTTTGTACAGTT185900.1426069204783461No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA184310.14138720555870884No Hit
TGAAATGTTTAGGAC183830.14101898973391266No Hit
TGAGGTAGTAGATTGTATAGTC181740.13941571666344607No Hit
CAACGGAATCCCAAAAGCAGCTG175960.13498178443985895No Hit
TGAGGTAGTAGGTTGTATAG175390.1345445281479135No Hit
TGAGGTAGTAGTTTGTGCTGT174920.13418398348613395No Hit
TGTAAACATCCCCGACTGGAAG174420.13380042533530462No Hit
TTCAAGTAATTCAGGATAGGT172700.13248098529645172No Hit
TGAGATGAAGCACTGTAGCTC166520.12774020655220117No Hit
TGAGGTAGTAGTTTGTGCTGTC165580.127019117228642No Hit
TACAGTACTGTGATAACTGAAG159430.12230135197344119No Hit
TGAGGTAGTAGGTTGTGTGGTTT157440.12077479053314046No Hit
TCAGTGCATCACAGAACTTTGT153270.11757591555522381No Hit
AAGCTGCCAGTTGAAGAACT153060.11741482113187551No Hit
TGAGGTAGTAGGTTGTGTGGTC151130.11593428666967426No Hit
TAGCTTATCAGACTGATGTTGACA147530.11317266798370307No Hit
GGCTGGTCCGATGGTAGTGGGT146180.11213706097646388No Hit
AGTTGGTCCGAGTGTTGT146060.11204500702026485No Hit
TCACAGTGAACCGGTCTCTTT142240.10911462274792873No Hit
AAAAGCTGGGTTGAGAGGGCGT140830.10803298876259002No Hit
CATTGCACTTGTCTCGGTCTG140010.1074039533952299No Hit
TGAGGTAGTAGGTTGTATAGC139870.10729655711299768No Hit
GGCTGGTCCGATGGTAGTGGGTT138210.10602314405224429No Hit
AGAGGTAGTAGGTTGCATAGTT137300.10532506821773492No Hit
TGAGGTAGTAGATTGTATAGTTT137010.1051026044902539No Hit
ACCAATATTACTGTGCTGCTT135180.10369878165821854No Hit
TCGTACCGTGAGTAATAATGCA134630.10327686769230629No Hit
TGGAGTGTGACAATGGTGTTTGT133760.10260947650986324No Hit
GGCTGGTCCGATGGTAGTGGGTTATC132180.10139743275324253No Hit
TCCTGTACTGAGCTGCCCCGAA132170.10138976159022595No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT131760.1010752439065459No Hit
AGCTACATCTGGCTACTGGGTCTCT131590.10094483413526394No Hit
TGAGAACTGAATTCCATGGGT130810.10034648341997017No Hit
TAGTGGGTTATCAGAACT130460.10007799271438964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)