FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10071081
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT132548413.161288247011418No Hit
TCAGTGCACTACAGAACTTTGT3008872.987633601596492No Hit
AAACCGTTACCATTACTGAGC2217002.2013525658268462No Hit
TATTGCACTTGTCCCGGCCTGT1824681.8118015335195894No Hit
TCCTGTACTGAGCTGCCCCGAG1488301.4777956805232726No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1289611.2805080209363822No Hit
CGCGACCTCAGATCAGA1228121.2194520131453614No Hit
TCCTGTACTGAGCTGCCCCGA1103541.0957512902537472No Hit
TGAGGTAGTAGGTTGTATAGT1080951.0733207289267161No Hit
TGAGGTAGTAGGTTGTATAGTT898000.8916619774977482No Hit
TTCAAGTAATCCAGGATAGGCT862140.8560550749219473No Hit
CATTGCACTTGTCTCGGTCTGA686840.6819923303168747No Hit
TGAGGGGCAGAGAGCGAGACTTT677880.6730955693832668No Hit
AAGCTGCCAGTTGAAGAACTGT649230.6446477791212284No Hit
AAACCGTTACCATTACTGAG637590.633089933444086No Hit
TGAGGTAGTAGATTGTATAGT626610.6221874295321426No Hit
TGAGGGGCAGAGAGCGAGACT582430.578319248946563No Hit
TGAGGTAGTAGATTGTATAGTT577780.5737020683281169No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT556570.5526417670555921No Hit
CCCATAAAGTAGAAAGCACT547290.5434272646600697No Hit
TCAGTGCACTACAGAACTTTGC526920.5232010347250707No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT484080.4806633965112583No Hit
TCCTGTACTGAGCTGCCCCGAGA433420.43036095132190877No Hit
TGAGGTAGTAGGTTGTGTGGTT426920.42390682787676914No Hit
TGAGGTAGTAGGTTGTGTGGT417550.41460296069508334No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC395470.39267879982297826No Hit
TATTGCACTTGTCCCGGCCTGC395050.39226176415421543No Hit
TGAGGTAGTAGTTTGTGCTGTT391940.3891737143212332No Hit
TGAGGTAGTAGTTTGTACAGT390860.38810133688727155No Hit
TAGCTTATCAGACTGATGTTGAC380430.3777449511129937No Hit
TAGCTTATCAGACTGATGTTGA377630.3749647133212413No Hit
TGAGGTAGTAGGTTGTATAG365120.3625430080445188No Hit
TCCTGTACTGAGCTGCCCCG358850.35631726127513025No Hit
AAAAGCTGGGTTGAGAGGGCGA345960.3435182380123842No Hit
CGCGACCTCAGATC337670.33528674826466No Hit
AAATGGATTTTTGGAGCAGGGAGA331560.3292198722262287No Hit
AAACCGTTACCATTACTGAGTT327850.3255360571521568No Hit
CGCGACCTCAGATCAGACGT315200.3129753399858466No Hit
TCCTGTACTGAGCTGCCCCGAGT299380.2972669964624453No Hit
TGGCTCAGTTCAGCAGGAAC294760.29267960410605376No Hit
AAACCGTTACCATTACTG289860.287814187970487No Hit
TTCACAGTGGCTAAGTTCCG289790.2877446820256932No Hit
TGGAGTGTGACAATGGTGTTT262130.26027990441145293No Hit
CATTGCACTTGTCTCGGTCTG232180.23054128946038666No Hit
TAGCTTATCAGACTGATGTTG231030.22939940608163117No Hit
TGAGGGGCAGAGAGCGAGACTT226830.22522904939400248No Hit
TGAGGTAGTAGTTTGTGCTGT221060.2194997736588555No Hit
TGAGATGAAGCACTGTAGCT214890.2133733210963153No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG205440.20399001854915078No Hit
TATTGCACTTGTCCCGGCCTG202280.20085232161274444No Hit
TGAGGTAGTAGGTTGTGTGG201790.20036577999918778No Hit
TTCAAGTAATTCAGGATAGGT196800.19541099907745751No Hit
TGAGGTAGTAGTTTGTACAGTT194590.19321659710611006No Hit
AAATGGATTTTTGGAAATAGGAGA193120.19175697226544003No Hit
TCGTACCGTGAGTAATAATGCG187680.1863553674128924No Hit
TGAGGTAGTAGGTTGTATAGC182380.18109277444993244No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC181760.18047715036747297No Hit
TGTAAACATCCCCGACTGGAAG181620.18033813847788535No Hit
TTCAAGTAATCCAGGATAGGCC179370.17810401882379856No Hit
TGTAAACATCCTTGACTGGAAGCT178890.17762740663092672No Hit
AAATGGATTTTTGGAGCAGGGA178700.17743874763791492No Hit
AAACCGTTACCATTACTGAGA175130.17389394445343057No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT174270.17304001427453516No Hit
TGAGGTAGTAGATTGTATAG166750.1655730899195429No Hit
GGCTGGTCCGATGGTAGT161190.16005233201877733No Hit
AAATGGATTTTTGGAGCAGGGAG159430.15830475397824723No Hit
TGAGGTAGTAGGTTGTATAGTC157890.15677562319278338No Hit
AAATGGATTTTTGGAGCAGGG154240.15315138464282038No Hit
TCCTGTACTGAGCTGCCCCGAA149430.14837533329341707No Hit
TGTAAACATCCCCGACTGGAAGCT148870.14781928573506656No Hit
TGAGAACTGAATTCCATGGGTT148020.14697528497685602No Hit
TAGCAGCACGTAAATATTGGCG147030.14599227232905782No Hit
TACAGTACTGTGATAACTGAAG144080.14306309322703292No Hit
AAATGGATTTTTGGAGCAGGGAGAT142550.1415438918622539No Hit
TGAGATGAAGCACTGTAGCTC141000.14000483165610525No Hit
GCATTGGTGGTTCAGTGGTAGAATT140400.13940906641501544No Hit
GGCTGGTCCGATGGTAGTGGGT137060.13609263990628215No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC136810.1358444043891614No Hit
AAGCTGCCAGTTGAAGAACT136000.13504012131369014No Hit
TGAGGTAGTAGGTTGTAT135580.1346230856449273No Hit
TGGCTCAGTTCAGCAGGAACAG132440.13150524754989062No Hit
TGGAGAGAAAGGCAGTTCCTGA123360.12248933356806484No Hit
TGAAAAGAACTTTGAAGAGAG122660.12179427412012674No Hit
AAACCGTTACCATTACTGAGG122470.12160561512711496No Hit
AGAGGTAGTAGGTTGCATAGT120920.12006655492096628No Hit
AAGCTGCCAGTTGAAGAACTGC119090.11824947093564235No Hit
AAAAGCTGGGTTGAGAGGGCG117930.11709765813620206No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA117370.11654161057785158No Hit
ACCAATATTACTGTGCTGCTT117070.11624372795730667No Hit
GGCTGGTCCGATGGTAGTG116720.1158961982333376No Hit
AGCTCGGTCTGAGGCCCCTCAGT116250.1154295154611506No Hit
AACATTCAACGCTGTCGGTGA113740.11293723086925823No Hit
GGCTGGTCCGATGGTAGTGGGTT113740.11293723086925823No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC113350.11254998346254985No Hit
AGAGGTAGTAGGTTGCATAGTT109900.10912433332628343No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA108500.10773421443040722No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT108450.10768456732698306No Hit
AGACCGTTACCATTACTGAGT107650.10689021367219667No Hit
TGAGGGGCAGAGAGCGAGACC107070.10631430727247651No Hit
TGAGGTAGTAGATTGTATAGC105640.10489440011454579No Hit
AAAAGCTGGGTTGAGAGGGCGT101710.10099213778540755No Hit
AGGAAACTCTGGTG101530.1008134082130806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)