FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11962958
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT6049285.056675782026486No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC5539334.630401611374043No Hit
TCAGTGCACTACAGAACTTTGT3729643.1176570209474948No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT3174792.653850326984346No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT2684502.2440102188773046No Hit
TTCAAGTAATCCAGGATAGGCT1740331.4547656190049318No Hit
AAACCGTTACCATTACTGAGC1318621.1022524696651113No Hit
TGAGGGGCAGAGAGCGAGACTTT1196831.000446545076895No Hit
AAGCTGCCAGTTGAAGAACTGT1022720.8549056178246216No Hit
TAGCTTATCAGACTGATGTTGA945900.7906907305032752No Hit
TGAGGTAGTAGTTTGTGCTGTT937720.7838529567687189No Hit
TGGCTCAGTTCAGCAGGAAC931950.7790297349535122No Hit
TCCTGTACTGAGCTGCCCCGAG883390.7384377676491048No Hit
TCAGTGCACTACAGAACTTTGC861540.7201730541894404No Hit
TTCACAGTGGCTAAGTTCCG856670.7161021546677669No Hit
TAGCTTATCAGACTGATGTTGAC791150.6613330916985581No Hit
CCCATAAAGTAGAAAGCACT756460.632335246851155No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC752890.6293510350868071No Hit
TGGAGTGTGACAATGGTGTTT751300.628021932368232No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC734680.6141290473476544No Hit
TGAGGTAGTAGGTTGTATAGT664020.5550633881687121No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT658540.550482581314755No Hit
TATTGCACTTGTCCCGGCCTGT628730.5255639951256202No Hit
TAGCTTATCAGACTGATGTTG559500.46769369247973624No Hit
TGAGGTAGTAGTTTGTACAGT550000.4597525127146647No Hit
TGAGGTAGTAGGTTGTGTGGT529750.4428252611101703No Hit
TGAGGTAGTAGGTTGTGTGGTT529230.44239058600724No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC518370.4333125636652741No Hit
TCCTGTACTGAGCTGCCCCGA517280.43240141777643953No Hit
TGAGGTAGTAGATTGTATAGT502340.4199128677037903No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT494980.4137605431700086No Hit
TTCAAGTAATCCAGGATAGGCC466150.38966115236716536No Hit
TGAGGGGCAGAGAGCGAGACT441060.36868807865078185No Hit
TGAGGTAGTAGGTTGTATAGTT431920.3610478278031236No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC429620.35912522638631683No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT428150.3578964333068794No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT428150.3578964333068794No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT425600.35576485347520237No Hit
TGAGGTAGTAGATTGTATAGTT418410.3497546342635325No Hit
AAACCGTTACCATTACTGAG401350.33549394723278303No Hit
CGCGACCTCAGATCAGACGT400960.3351679409055854No Hit
TGTAAACATCCTTGACTGGAAGCT370420.30963913774502927No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA348210.29107349536795163No Hit
TCCTGTACTGAGCTGCCCCGAGT346770.2898697796982987No Hit
TCGTACCGTGAGTAATAATGCG339370.28368401861813775No Hit
AAACCGTTACCATTACTGAGTT335510.2804573918925403No Hit
CATTGCACTTGTCTCGGTCTGA315450.2636889638833473No Hit
TACAGTATAGATGATGTACT308410.25780413172059957No Hit
TGAGATGAAGCACTGTAGCT299070.24999669814104505No Hit
AAATGGATTTTTGGAGCAGGG298710.24969576922363185No Hit
AAATGGATTTTTGGAGCAGGGAGA296180.24758090766514437No Hit
AAATGGATTTTTGGAGCAGGGA290250.2426239396644208No Hit
TGAGAACTGAATTCCATGGGTT285750.23886232819675535No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT283380.23688121282378488No Hit
TCCTGTACTGAGCTGCCCCGAGA275240.23007687563560783No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT257110.2149217609892135No Hit
AAGCTGCCAGTTGAAGAACTGC251390.21014033485698103No Hit
TGAGGTAGTAGTTTGTGCTGT250950.2097725328468093No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA247180.20662113835056514No Hit
TACAGTACTGTGATAACTGAAG242350.20258367537527092No Hit
AAAAGCTGGGTTGAGAGGGCGA219320.18333258379741865No Hit
TGAGGTAGTAGTTTGTGCTGTC213900.17880193176303052No Hit
AAATGGATTTTTGGAGCAGGGAG210080.1756087415838123No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA208170.1740121464942032No Hit
TGAGGGGCAGAGAGCGAGACTT207560.17350223916191967No Hit
TGAGGGGCAGAGAGCGAGACTTC207390.17336013383980783No Hit
TGAGATGAAGCACTGTAGCTC201030.16804372296550735No Hit
TTCAAGTAATTCAGGATAGGT194900.16291957223288756No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC193750.16195827152448417No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG189620.15850594811082677No Hit
TGTAAACATCCCCGACTGGAAG188970.157962604232164No Hit
TCAGTGCATCACAGAACTTTGT187900.1570681766165191No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA184210.15398365521303342No Hit
AGCTCGGTCTGAGGCCCCTCAGT184060.15385826816411124No Hit
TACAGTACTGTGATAACTGA180790.1511248304976077No Hit
TCCTGTACTGAGCTGCCCCG179600.15013009324282506No Hit
TTCACAGTGGCTAAGTTCCA179200.14979572777903258No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCC177060.14800687254774278No Hit
TATTGCACTTGTCCCGGCCTGC176320.1473882964397267No Hit
CCCATAAAGTAGAAAGCACC172120.14387745906990562No Hit
TGAGGTAGTAGTTTGTACAGTT165020.13794247208758903No Hit
TGAGGTAGTAGGTTGTGTGG163850.13696445310599603No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG160450.1341223466637599No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCT157780.1318904571929451No Hit
AACATTCAACGCTGTCGGTGA155220.1297505182246732No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC155150.12969200426850952No Hit
CATTGCACTTGTCTCGGTCTG154890.12947466671704438No Hit
TGTAAACATCCCCGACTGGAAGCT149140.12466816317502744No Hit
TGGAGTGTGACAATGGTGTTC148660.12426692461847647No Hit
TGAGGTAGTAGGTTGTATAGC148350.1240077913840373No Hit
TTCAAGTAATCCAGGATAGGCA148130.12382389037895142No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC138050.11539788069138085No Hit
TACAGTACTGTGATAACTGAA137110.1146121218514685No Hit
TAGCTTATCAGACTGATGTTGAT136790.11434462948043453No Hit
AAATGGATTTTTGGAGCAGGGAGATGGAAT136710.11427775638767601No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG136400.11401862315323684No Hit
GGCTGGTCCGATGGTAGTGGGTTATC135580.11333317395246226No Hit
CAACGGAATCCCAAAAGCAGCT134910.11277311180060985No Hit
TGAGGTAGTAGGTTGTGTGGC133610.11168642404328429No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCA131850.11021521600259734No Hit
TGGCTCAGTTCAGCAGGAACAG128680.10756536970204192No Hit
TGAGGTAGTAGGTTGTGTGGTC127600.10666258294980221No Hit
AAATGGATTTTTGGAGCAGGGAGAT126970.10613595734432904No Hit
GCATTGGTGGTTCAGTGGTAGAATT126280.10555917691928703No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCG122390.1023074727839051No Hit
TGAGGTAGTAGTTTGTACAGC122160.10211521264222444No Hit
TGGCTCAGTTCAGCAGGAAT120110.10040158964028797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)