FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13384210
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT8769576.55217603429713No Hit
TCAGTGCACTACAGAACTTTGT3227042.4110799217884358No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC2218701.6576996326268043No Hit
AAACCGTTACCATTACTGAGC1872441.3989917970504049No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1834651.370757033848094No Hit
TATTGCACTTGTCCCGGCCTGT1631861.2192426747637701No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1571311.174002798820401No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1152600.8611640134158086No Hit
TCCTGTACTGAGCTGCCCCGAG1126230.8414616925466651No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT1053150.7868600388069225No Hit
TGAGGGGCAGAGAGCGAGACTTT1051280.785462870053593No Hit
TTCAAGTAATCCAGGATAGGCT1028820.7686819020323201No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC837020.6253787111828042No Hit
TCCTGTACTGAGCTGCCCCGA827310.6181238937524142No Hit
AAGCTGCCAGTTGAAGAACTGT827300.6181164222617547No Hit
TCAGTGCACTACAGAACTTTGC725600.5421313622544774No Hit
CCCATAAAGTAGAAAGCACT704890.5266579050986199No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT653270.4880900703141986No Hit
CATTGCACTTGTCTCGGTCTGA651510.48677508795812385No Hit
AAACCGTTACCATTACTGAGTT628200.46935904323079214No Hit
CGCGACCTCAGATC570460.42621865616274696No Hit
TAGCTTATCAGACTGATGTTGA566030.42290878580058144No Hit
TGAGGTAGTAGGTTGTATAGT534310.39920921742859683No Hit
TGAGGGGCAGAGAGCGAGACT534200.39912703103134217No Hit
TGGAGTGTGACAATGGTGTTT519850.38840544193493676No Hit
CGCGACCTCAGATCAGACGT508580.37998507196166226No Hit
TGAGGTAGTAGGTTGTATAGTT491070.36690249181684986No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC475620.3553590387478977No Hit
AAACCGTTACCATTACTGAG465610.34788007659772224No Hit
TATTGCACTTGTCCCGGCCTGC444090.3318014286984439No Hit
TGAGGTAGTAGTTTGTGCTGTT435300.32523398840872936No Hit
TGGCTCAGTTCAGCAGGAAC432010.3227758679817486No Hit
TGAGGTAGTAGGTTGTGTGGTT431610.322477008355368No Hit
TAGCTTATCAGACTGATGTTGAC418540.3127117700633807No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC409990.30632364554949454No Hit
TGAGGTAGTAGGTTGTGTGGT409930.3062788166055374No Hit
TGAGGTAGTAGTTTGTACAGT407990.3048293474175913No Hit
TCCTGTACTGAGCTGCCCCGAGT398330.29761188744049893No Hit
TGAGGTAGTAGATTGTATAGTT356610.26644082840899835No Hit
TCCTGTACTGAGCTGCCCCGAGA350740.2620550633918625No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT342610.255980741485676No Hit
TTCACAGTGGCTAAGTTCCG340670.2545312722977299No Hit
TGAGGTAGTAGATTGTATAGT338870.2531864039790171No Hit
TGTAAACATCCTTGACTGGAAGCT333130.2488977683404549No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT331970.24803107542395106No Hit
TGAGATGAAGCACTGTAGCT308700.23064491665925743No Hit
GCATTGGTGGTTCAGTGGTAGAATT294200.2198112552029593No Hit
CGCGACCTCAGATCAGA292390.21845891539358692No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC274890.20538380673943402No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT269400.20128195836735976No Hit
TTCAAGTAATCCAGGATAGGCC265000.1979945024771727No Hit
TGAGGGGCAGAGAGCGAGACTT261910.1956858118633823No Hit
TCCTGTACTGAGCTGCCCCG260570.19468463211500717No Hit
TAGCTTATCAGACTGATGTTG240990.18005545340367493No Hit
GGCTGGTCCGATGGTAGTGGGT229070.17114943653753192No Hit
GGCTGGTCCGATGGTAGT225450.16844475691878716No Hit
TGTAAACATCCCCGACTGGAAG225250.1682953271055968No Hit
TGAGGTAGTAGTTTGTGCTGT222560.16628549611818702No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCT220790.1649630422714527No Hit
AAAAGCTGGGTTGAGAGGGCGA217420.1624451499191958No Hit
TCAGTGCATCACAGAACTTTGT217050.16216870476479373No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC209880.1568116459619208No Hit
GGAATACCGGGTGCTGTAGGCTT205600.15361384795964797No Hit
CATTGCACTTGTCTCGGTCTG202950.15163390293487625No Hit
TGAGATGAAGCACTGTAGCTC202610.1513798722524527No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG201860.150819510452989No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG200420.1497436157980187No Hit
GGCTGGTCCGATGGTAGTGGGTT200090.14949705660625467No Hit
AAGCTGCCAGTTGAAGAACTGC193300.14442391444844335No Hit
TGAGAACTGAATTCCATGGGTT192140.1435572215319395No Hit
TGTAAACATCCCCGACTGGAAGCT190770.1425336273115858No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC187470.14006803539394555No Hit
TAGCAGCACGTAAATATTGGCG186800.139567445519758No Hit
AAATGGATTTTTGGAGCAGGGAGA185950.1389323688136991No Hit
AGCTCGGTCTGAGGCCCCTCAGT184080.13753520006036965No Hit
TCGTACCGTGAGTAATAATGCG182360.13625010366693288No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT180180.1346213187031584No Hit
TGAGGGGCAGAGAGCGAGACTTC178030.1330149482113625No Hit
AGGAAACTCTGGTG174340.13025796815800111No Hit
TACAGTACTGTGATAACTGAAG165710.12381007171883883No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC163550.12219622973638339No Hit
TGAGGTAGTAGTTTGTACAGTT162230.12120999296932727No Hit
TGAGGTAGTAGGTTGTGTGG159750.11935706328576733No Hit
GCATTGGTGGTTCAGTGGTAGAATTCT151490.11318561200100716No Hit
TCCTGTACTGAGCTGCCCCGAA151080.112879280883967No Hit
CCCATAAAGTAGAAAGCACC150890.11273732256143619No Hit
TGAGGTAGTAGGTTGTATAG150810.11267755063616007No Hit
AAACCGTTACCATTACTGAGA148820.11119072399491639No Hit
GGCTGGTCCGATGGTAGTGGGTTATC147230.11000275698005335No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA144810.10819465624045049No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA144460.10793315406736745No Hit
AAACCGTTACCATTACTG143990.1075819940063702No Hit
TGTCAGTTTGTCAAATACCCCAA143950.10755210804373214No Hit
GGCTGGTCCGATGGTAGTG139320.10409280786837624No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA136080.10167204489469307No Hit
TCAAGGCCGAGAGCTGATGACGAGTCGT136020.10162721595073597No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)