FastQCFastQC Report
Wed 8 May 2024
EGAF00002167631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12787136
Total Bases301.7 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT7571145.920903633151317No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC6217574.862363237553741No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT2495421.9515081406813846No Hit
TCCTGTACTGAGCTGCCCCGAG2398161.875447324561184No Hit
TCAGTGCACTACAGAACTTTGT2178051.7033133924594217No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT1519201.1880690093544013No Hit
TGGAGTGTGACAATGGTGTTT1416841.1080198099089584No Hit
TCCTGTACTGAGCTGCCCCGA1416541.1077851991251209No Hit
TTCAAGTAATCCAGGATAGGCT1222300.9558825369496343No Hit
AAACCGTTACCATTACTGAGC1113230.8705858763056872No Hit
TGAGGGGCAGAGAGCGAGACTTT1091550.8536313369936787No Hit
TATTGCACTTGTCCCGGCCTGT1049140.8204651925184812No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC986600.77155666444777No Hit
TGAGGTAGTAGGTTGTGTGGTT858690.6715264465788117No Hit
AAGCTGCCAGTTGAAGAACTGT795020.6217342178889784No Hit
TGAGGTAGTAGGTTGTGTGGT786210.6148444812036097No Hit
CGCGACCTCAGATCAGA784050.6131552835599778No Hit
TGGCTCAGTTCAGCAGGAAC775310.6063202893908378No Hit
TCCTGTACTGAGCTGCCCCGAGA745800.5832424086206638No Hit
TAGCTTATCAGACTGATGTTGAC743390.5813577019905004No Hit
TGAGGTAGTAGGTTGTATAGT736800.5762040851055311No Hit
TAGCTTATCAGACTGATGTTGA724680.5667258094384856No Hit
TGAGGTAGTAGTTTGTGCTGTT701400.5485200126126757No Hit
AAACCGTTACCATTACTGAG688880.53872892256718No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC655470.5126011016071151No Hit
TTCACAGTGGCTAAGTTCCG639970.5004795444421644No Hit
CATTGCACTTGTCTCGGTCTGA604660.4728658551844604No Hit
TGAGGGGCAGAGAGCGAGACT597400.4671882742155867No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG590940.46213632200361365No Hit
TGAGGTAGTAGGTTGTATAGTT574110.44897465703031547No Hit
TCCTGTACTGAGCTGCCCCGAGT541290.42330823727846484No Hit
TCGTACCGTGAGTAATAATGCG535350.4186629437584773No Hit
TAGCTTATCAGACTGATGTTG516640.40403105120646254No Hit
TCCTGTACTGAGCTGCCCCG497610.3891489071516875No Hit
CCCATAAAGTAGAAAGCACT495560.38754573346212945No Hit
TGAGGTAGTAGATTGTATAGT490040.38322889503951474No Hit
TGAGGTAGTAGTTTGTACAGT487610.38132854769042884No Hit
CGCGACCTCAGATC480660.3758933978648542No Hit
TGAGGTAGTAGATTGTATAGTT446610.34926507389926875No Hit
CGCGACCTCAGATCAGACGT391290.3060028453595864No Hit
AAAAGCTGGGTTGAGAGGGCGA389190.3043605698727221No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC385360.30136537219906No Hit
TGTAAACATCCCCGACTGGAAG352530.2756911320877482No Hit
TCAGTGCACTACAGAACTTTGC347590.27182787451388646No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAA333770.26102013773842714No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAA329930.25801711970530383No Hit
TGAGGTAGTAGGTTGTGTGG325830.2548107723261878No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC299080.23389131076732117No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT297060.23231159815614694No Hit
TACAGTACTGTGATAACTGAAG295340.23096649632881044No Hit
CATTGCACTTGTCTCGGTCTG293010.2291443525743372No Hit
TGAGGTAGTAGGTTGTATAG286130.22376394526499133No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT273810.21412926240872077No Hit
AAACCGTTACCATTACTGAGTT266740.20860026826961098No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCT256730.20077208844889116No Hit
AAACCGTTACCATTACTG244900.19152060320622225No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT238230.1863044234455628No Hit
TGAGGGGCAGAGAGCGAGACTT234730.1835672976341223No Hit
TGTAAACATCCTTGACTGGAAGCT228040.17833547715454032No Hit
TTCAAGTAATCCAGGATAGGCC219940.17200098599092087No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGA210810.16486099780279181No Hit
TGTAAACATCCCCGACTGGAAGCT210450.1645794648621865No Hit
AAATGGATTTTTGGAGCAGGGAGA209460.1638052492755219No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC201680.15772100961466273No Hit
TGAGGTAGTAGTTTGTGCTGT201580.15764280602005015No Hit
TGAGAACTGAATTCCATGGGTT190490.1489700273775144No Hit
GGCTGGTCCGATGGTAGTGGGTTATC188920.14774223094209682No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA185260.14487997937927619No Hit
CTAGACTGAAGCTCCTTGAGG183780.14372256617900991No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT181890.14224451824083204No Hit
TATTGCACTTGTCCCGGCCTGC178760.1397967457294581No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT177920.13913983553471237No Hit
AAAAGCTGGGTTGAGAGGGCG173970.13605079354751526No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC167520.13100666169500347No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAC166190.1299665538866561No Hit
TGAGGTAGTAGTTTGTACAGTT166130.12991963172988855No Hit
CAACGGAATCCCAAAAGCAGCTG164860.12892644607830872No Hit
TGAGGTAGTAGATTGTATAG163180.12761262568881726No Hit
TGGCTCAGTTCAGCAGGAACAG160450.12547766755589368No Hit
TAGCAGCACGTAAATATTGGCG157020.12279528426068198No Hit
TTCAAGTAATTCAGGATAGGT155870.12189594292263725No Hit
TACAGTACTGTGATAACTGA155300.12145018243334552No Hit
AACATTCAACGCTGTCGGTGA155110.12130159560358161No Hit
TGAGATGAAGCACTGTAGCTC152130.11897112848412655No Hit
TATTGCACTTGTCCCGGCCTG151570.11853318835429608No Hit
AGGGCTGGGTCGGTCGGGCTGGGGC147320.11520953558326118No Hit
CGCGACCTCAGATCAGAC145260.1135985415342419No Hit
GGCTGGTCCGATGGTAGTG144540.11303547565303129No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGCG143470.11219869719067664No Hit
TGGAGTGTGACAATGGTGTTC140920.11020450552805569No Hit
AGCTCGGTCTGAGGCCCCTCAGT140220.1096570803657676No Hit
GCATTGGTGGTTCAGTGGTAGAATT139200.10885940370071923No Hit
GGCTGGTCCGATGGTAGTGGGT138050.10796006236267448No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCT137220.10731097252739003No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCA136080.10641945154880655No Hit
TACAGTATAGATGATGTACT135580.10602843357574362No Hit
TCCTGTACTGAGCTGCCCCGAA132930.1039560383185101No Hit
AAGCTGCCAGTTGAAGAACTGC130260.10186800234235406No Hit
TGAGATGAAGCACTGTAGCT129410.10120327178814709No Hit
TGTAAACATCCCCGACTGGAAGC129070.1009373795664643No Hit
TGGAGAGAAAGGCAGTTCCTG128200.10025700829333481No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)