FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13222057
Sequences flagged as poor quality0
Sequence length14-50
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT9358727.078111976071499No Hit
CGCGACCTCAGATCAGA7056955.337255768901919No Hit
TCCTGTACTGAGCTGCCCCGA3524142.6653492720535086No Hit
TCCTGTACTGAGCTGCCCCGAG3354502.537048509169186No Hit
TATTGCACTTGTCCCGGCCTGT2433481.840470056966174No Hit
TGGAGTGTGACAATGGTGTTT1778401.3450252105251097No Hit
TCAGTGCACTACAGAACTTTGT1457441.1022793200785628No Hit
TTCAAGTAATCCAGGATAGGCT1451011.097416234100337No Hit
TAGCTTATCAGACTGATGTTGAC1395811.0556678132608264No Hit
AAACCGTTACCATTACTGAGC1238760.9368890181005874No Hit
TCCTGTACTGAGCTGCCCCG979880.7410949748590556No Hit
TCCTGTACTGAGCTGCCCCGAGA898300.6793950442053003No Hit
TGAGGGGCAGAGAGCGAGACTTT839380.6348331428309529No Hit
TAGCTTATCAGACTGATGTTGA816230.6173245206853971No Hit
TCGTACCGTGAGTAATAATGCG803450.6076588536866843No Hit
CGCGACCTCAGATCAGAC742690.5617053382843531No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC741260.5606238121647789No Hit
TAGCTTATCAGACTGATGTTG734150.5552464340457767No Hit
CGGTCGGGCTGGGGCGC677170.512151777896586No Hit
TATTGCACTTGTCCCGGCCTG637400.4820732507808732No Hit
TGAGGTAGTAGGTTGTGTGGTT636420.48133206504857756No Hit
AAGCTGCCAGTTGAAGAACTGT625010.47270254545113516No Hit
TGAGGTAGTAGGTTGTGTGGT613640.46410327833256204No Hit
TGAGGGGCAGAGAGCGAGACT595530.45040646852452687No Hit
CGCGACCTCAGATC572100.4326860790268867No Hit
CGCGACCTCAGATCAGACGT569320.4305835317454765No Hit
TGAGGTAGTAGGTTGTATAGTT542150.41003453547356516No Hit
TGGAGTGTGACAATGGTGTTTG531570.4020327548126589No Hit
TCCTGTACTGAGCTGCCCCGAGT525530.3974646305034081No Hit
TGAGGTAGTAGGTTGTGTGG512300.3874586231174166No Hit
AAACCGTTACCATTACTGAG483420.36561633337384647No Hit
CGGTCGGGCTGGGGCG476580.3604431594872114No Hit
TGGCTCAGTTCAGCAGGAAC448810.33944037603226185No Hit
CCCATAAAGTAGAAAGCACT392900.29715497369282257No Hit
TGAGGTAGTAGATTGTATAGTT386490.29230701395403147No Hit
AAACCGTTACCATTACTG366110.27689337597016866No Hit
CATTGCACTTGTCTCGGTCTGA354570.26816553581640135No Hit
TGAGGTAGTAGGTTGTATAG350340.2649663361759823No Hit
TGTAAACATCCCCGACTGGAAGC346010.26169150533839025No Hit
TGAGGTAGTAGTTTGTGCTGTT334550.25302417014236134No Hit
TATTGCACTTGTCCCGGCCTGC327420.24763166578392454No Hit
TGTAAACATCCCCGACTGGAAGCT327110.24739720907268817No Hit
TGTAAACATCCCCGACTGGAAG310430.2347819253842273No Hit
CGCGACCTCAGATCAGACG303110.2292457217511617No Hit
CTAGACTGAAGCTCCTTGAGG280320.2120093719154289No Hit
TACAGTACTGTGATAACTGAAG274590.20767570431741447No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT273690.2069950235428572No Hit
TGAGGTAGTAGGTTGTATAGT270890.20487735002201246No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC265630.2008991490507112No Hit
TTCACAGTGGCTAAGTTCCG263700.19943946694527181No Hit
AACCCGTAGATCCGATCTTGT261910.19808566851587467No Hit
AAAAGCTGGGTTGAGAGGGCGA259200.1960360630724856No Hit
TGAGGTAGTAGTTTGTACAGT246650.18654434782727075No Hit
TGAAAAGAACTTTGAAGAGAG240100.1815905044124375No Hit
TTCAAGTAATCCAGGATAGGCC232990.17621312629343527No Hit
TACCCTGTAGAACCGAATTTGT232740.17602404830050272No Hit
CATTGCACTTGTCTCGGTCTG225430.17049540778715447No Hit
AACATTCAACGCTGTCGGTGA210310.1590599707745928No Hit
TGAGATGAAGCACTGTAGCTC208730.15786499785925898No Hit
TCAGTGCACTACAGAACTTTGC206290.1560195966482371No Hit
AAGCTGCCAGTTGAAGAACT201320.15226072614873767No Hit
GGCTGGTCCGAAGGTAGTGAGTT174790.13219576953873363No Hit
TGGCTCAGTTCAGCAGGAACAG174510.13198400218664919No Hit
TGAGGGGCAGAGAGCGAGACTT173340.13109911717972475No Hit
AGCTCGGTCTGAGGCCCCTCAGT172290.13030498960940798No Hit
TGAGAACTGAATTCCATGGGTT167570.1267351971028411No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG161650.12225783023019793No Hit
GGCTGGTCCGATGGTAGTG161440.12209900471613457No Hit
TGAGGTAGTAGATTGTATAGT159090.12032167158256843No Hit
TGTAAACATCCCCGACTGGAA157960.11946703905451324No Hit
GTCGGTCGGGCTGGGGCGC153770.1162980918929634No Hit
CAACGGAATCCCAAAAGCAGCTG152920.11565522671699267No Hit
GGCTGGTCCGATGGTAGTGGGTT149090.11275855186526575No Hit
GGCTGGTCCGATGGTAGTGGGT145590.11011145996420979No Hit
TCCTGTACTGAGCTGCCCCGAA143570.10858370978131465No Hit
TCGTACCGTGAGTAATAATGC139490.10549795693665517No Hit
ACCAATATTACTGTGCTGCTT137170.10374331316224095No Hit
TGAGGTAGTAGTTTGTGCTGT133000.10058949224012571No Hit
CGCGACCTCAGATCAG132980.10057436600069113No Hit
TACAGTACTGTGATAACTGA132960.10055923976125651No Hit
AAAAGCTGGGTTGAGAGGGCG132650.10032478305002013No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)