FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13264348
Sequences flagged as poor quality0
Sequence length14-50
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT13132449.900554478817956No Hit
TCCTGTACTGAGCTGCCCCGA4041293.0467309814247936No Hit
TCCTGTACTGAGCTGCCCCGAG3942342.9721325164267403No Hit
TATTGCACTTGTCCCGGCCTGT2968372.237855942862778No Hit
TGGAGTGTGACAATGGTGTTT2093201.5780647492059166No Hit
TCAGTGCACTACAGAACTTTGT1973271.4876494494866992No Hit
AAACCGTTACCATTACTGAGC1898171.431031513950026No Hit
CGCGACCTCAGATCAGA1671401.2600694734486761No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1527811.1518168853832844No Hit
TTCAAGTAATCCAGGATAGGCT1092160.8233800862281357No Hit
TCCTGTACTGAGCTGCCCCG1067890.8050829185120897No Hit
TCCTGTACTGAGCTGCCCCGAGA1004770.7574967122394557No Hit
CGGTCGGGCTGGGGCGC975730.7356034386311336No Hit
TGAGGGGCAGAGAGCGAGACTTT937350.7066687333595288No Hit
TGAGGGGCAGAGAGCGAGACT888470.6698180717212787No Hit
TAGCTTATCAGACTGATGTTGAC780570.588472196296418No Hit
AAGCTGCCAGTTGAAGAACTGT745840.5622892282379806No Hit
CGGTCGGGCTGGGGCG731110.5511842723064865No Hit
TCGTACCGTGAGTAATAATGCG703840.530625402771399No Hit
TGAGGTAGTAGGTTGTGTGG701380.5287708072797849No Hit
TCCTGTACTGAGCTGCCCCGAGT650670.4905405075319194No Hit
TATTGCACTTGTCCCGGCCTG641700.4837780190929852No Hit
TAGCTTATCAGACTGATGTTGA640770.48307689152908234No Hit
TGAGGTAGTAGGTTGTGTGGT618410.4662196739711594No Hit
TAGCTTATCAGACTGATGTTG617140.4652622202010985No Hit
TGAGGTAGTAGGTTGTGTGGTT615860.46429722742497403No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT523870.394945910647097No Hit
TGAGGTAGTAGGTTGTATAGTT495890.37385177168150296No Hit
TGGAGTGTGACAATGGTGTTTG483490.3645034041627979No Hit
TATTGCACTTGTCCCGGCCTGC469690.35409957579520684No Hit
CGCGACCTCAGATCAGACGT452430.3410872513296545No Hit
CATTGCACTTGTCTCGGTCTGA445430.33580994708522427No Hit
AAACCGTTACCATTACTGAG412910.31129309936681393No Hit
CCCATAAAGTAGAAAGCACT406040.3061138022012089No Hit
TGGCTCAGTTCAGCAGGAAC400520.3019522708541724No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC396490.29891405141059324No Hit
AAACCGTTACCATTACTG391430.29509931434247655No Hit
TGAGGTAGTAGGTTGTATAG356260.26858463001724625No Hit
TGTAAACATCCCCGACTGGAAGCT347520.26199553871777187No Hit
TGAGGTAGTAGATTGTATAGTT321870.24265798816496673No Hit
CTAGACTGAAGCTCCTTGAGG311670.2349682019802255No Hit
AAAAGCTGGGTTGAGAGGGCGA309860.23360364188273708No Hit
TCAGTGCACTACAGAACTTTGC306900.23137209608794945No Hit
TGTAAACATCCCCGACTGGAAGC305800.23054280542096753No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG303620.22889930209913067No Hit
TGTAAACATCCCCGACTGGAAG296570.22358430282438307No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT291980.22012389904124954No Hit
TGAGGTAGTAGGTTGTATAGT284890.214778743742248No Hit
CGCGACCTCAGATC281070.21189884342600177No Hit
GCGCGGGACATGTGGCGTACGGAAG276220.20824242548521796No Hit
TCCTGTACTGAGCTGCCCCGAA275160.20744329084248997No Hit
CGGACAGGATTGACAGA271580.20474432667176704No Hit
TACAGTACTGTGATAACTGAAG261490.197137469553724No Hit
TGAGGTAGTAGTTTGTGCTGTT251500.1896060024963157No Hit
AAGCTGCCAGTTGAAGAACT251310.18946276138110973No Hit
TACCCTGTAGAACCGAATTTGT249270.18792480414416146No Hit
TTCACAGTGGCTAAGTTCCG246150.18557263425235826No Hit
GCGGGACATGTGGCGTACGGAAGAC244200.18410252806998129No Hit
AACCCGTAGATCCGATCTTGT241650.18218008152379597No Hit
CGCGACCTCAGATCAGAC238590.1798731456683736No Hit
TGGAGTGTGACAATGGTGTTC231470.17450537335118166No Hit
CGGTCGGGCTGGGGCGT231240.1743319762117218No Hit
CATTGCACTTGTCTCGGTCTG227970.17186672122896657No Hit
TGAGGGGCAGAGAGCGAGACTT217470.16395076486232116No Hit
AAACCGTTACCATTACTGAGG214560.16175691409785087No Hit
GTCGGTCGGGCTGGGGCGC208090.1568791771747846No Hit
TGGCTCAGTTCAGCAGGAACAG206270.15550707807123273No Hit
AACATTCAACGCTGTCGGTGA199310.15025992985105638No Hit
TTCAAGTAATCCAGGATAGGCC186000.14022551278057543No Hit
TGAGATGAAGCACTGTAGCTC179600.13540054889995348No Hit
GCGGGACATGTGGCGTACGGAAGA179440.1352799248029379No Hit
TGAGGTAGTAGTTTGTACAGT178750.13475973338455835No Hit
AAACCGTTACCATTACTGAGA177440.13377212359024357No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC176710.13322177614761013No Hit
GGGGACGGACTGGGAACGGCTCC175850.13257342162615154No Hit
GTCGGTCGGGCTGGGGCG169400.12771076271521223No Hit
GCGGGACATGTGGCGTACGGAAG167620.12636881963591426No Hit
CGGTCGGGCTGGGGC166450.12548675592648806No Hit
TGAGAACTGAATTCCATGGGTT166030.12517011767182223No Hit
TGTAAACATCCTTGACTGGAAGCT160160.12074472111256429No Hit
ACCAATATTACTGTGCTGCTT159640.12035269279726377No Hit
CGCGACCTCAGATCAGACG156770.11818899805704736No Hit
TCCTGTACTGAGCTGCCCCA153550.11576143810460944No Hit
TGAGGTAGTAGATTGTATAGT153210.11550511189845139No Hit
AGCTCGGTCTGAGGCCCCTCAGT152260.11478890632242159No Hit
TATTGCACTTGTCCCGGCCTGA150140.11319063703696555No Hit
TGAGGTAGTAGTTTGTGCTGT145520.10970761623564158No Hit
GGCTGGTCCGATGGTAGTGGGTTATC144730.10911203475662731No Hit
TGAGGGGCAGAGAGCGAGACC143380.10809426893805861No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC141660.10679755989514148No Hit
TGTAAACATCCCCGACTGGAA140340.1058024110947632No Hit
AAAAGCTGGGTTGAGAGGGCG137320.10352563126359471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)