FastQCFastQC Report
Wed 8 May 2024
EGAF00002167637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12467266
Total Bases280.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT11198918.982651047952293No Hit
TCCTGTACTGAGCTGCCCCGA2357511.890959894494912No Hit
TATTGCACTTGTCCCGGCCTGT2273841.8238481476211383No Hit
AAACCGTTACCATTACTGAGC2089551.6760290507959001No Hit
TTCAAGTAATCCAGGATAGGCT1860671.4924442937208526No Hit
TCAGTGCACTACAGAACTTTGT1763091.4141753292181303No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1656701.3288398595169142No Hit
CGCGACCTCAGATCAGA1561011.25208686491489No Hit
TCCTGTACTGAGCTGCCCCGAG1490341.1954024242363963No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT1463681.1740184255313073No Hit
TGAGGTAGTAGGTTGTATAGT1226960.9841452007200294No Hit
TGAGGTAGTAGGTTGTGTGGT1203840.9656006377019629No Hit
TGAGGGGCAGAGAGCGAGACTTT1109210.8896978696050923No Hit
TGAGGTAGTAGGTTGTGTGGTT1058930.8493682576436565No Hit
TAGCTTATCAGACTGATGTTGAC999960.8020683925409148No Hit
TGAGGTAGTAGGTTGTATAGTT992210.7958521138475749No Hit
TGAGGTAGTAGATTGTATAGTT852460.6837585722483182No Hit
AAGCTGCCAGTTGAAGAACTGT774600.6213070291433583No Hit
TGAGGGGCAGAGAGCGAGACT765520.6140239568161937No Hit
TGAGGTAGTAGATTGTATAGT763700.6125641339488546No Hit
TGAGGTAGTAGTTTGTGCTGTT689070.5527033753831835No Hit
TCCTGTACTGAGCTGCCCCGAGT614850.49317147801290195No Hit
TGGAGTGTGACAATGGTGTTT601620.48255968870801347No Hit
TCCTGTACTGAGCTGCCCCGAGA570800.45783895202043495No Hit
TCCTGTACTGAGCTGCCCCG539690.43288560619465405No Hit
TATTGCACTTGTCCCGGCCTGC536540.4303589896934901No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT496110.39793006742617026No Hit
TCGTACCGTGAGTAATAATGCG479670.38474353559152424No Hit
TGAGGTAGTAGTTTGTACAGT450170.3610815715330049No Hit
TTCAAGTAATCCAGGATAGGCC418080.3353421672401952No Hit
TGTAAACATCCCCGACTGGAAGCT394050.316067692788459No Hit
TAGCTTATCAGACTGATGTTGA384980.30879264146606No Hit
TGAGGTAGTAGGTTGTGTGG360720.289333683904715No Hit
TCAGTGCACTACAGAACTTTGC355420.28508255137894706No Hit
CCCATAAAGTAGAAAGCACT344040.2759546479556945No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC330720.2652706696079156No Hit
TAGCTTATCAGACTGATGTTG316460.25383271681217034No Hit
TAGCTTATCAGACTGATGTTGAT308180.24719132486625378No Hit
AAACCGTTACCATTACTGAG298630.239531265315106No Hit
TGGCTCAGTTCAGCAGGAAC298160.2391542780911228No Hit
AAACCGTTACCATTACTGAGA281160.22551856998960315No Hit
TATTGCACTTGTCCCGGCCTG280340.22486084759882397No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT275070.2206337780873529No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG255680.20508104984685496No Hit
TGAGGTAGTAGGTTGTGTGGC247890.19883268713445273No Hit
CGCGACCTCAGATCAGACGT244550.1961536715427424No Hit
TGAGGTAGTAGGTTGTGTGGTC238990.19169399289306893No Hit
TCCTGTACTGAGCTGCCCCGAA237110.19018604399713618No Hit
AAGCTGCCAGTTGAAGAACT235700.1890550823251866No Hit
AGCTCGGTCTGAGGCCCCTCAGT234610.18818079280573624No Hit
TGAGGTAGTAGGTTGTATAGC234330.18795620467229943No Hit
AAAAGCTGGGTTGAGAGGGCGA232400.1864081507525387No Hit
CATTGCACTTGTCTCGGTCTGA218520.1752749961378862No Hit
GGTGGGCGTAGCTC217930.1748017568567158No Hit
AAACCGTTACCATTACTG216860.17394350934679664No Hit
TGAGGTAGTAGGTTGTATAGTC216180.17339808102273585No Hit
TGAGGTAGTAGGTTGTATAG211750.16984477591157515No Hit
TGAGGGGCAGAGAGCGAGACTT209530.16806411285361203No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCT207940.16678877309588166No Hit
TGAGGGGCAGAGAGCGAGACTTC203960.16359641319917292No Hit
TTCAAGTAATCCAGGATAGGCA200670.1609575026312906No Hit
CTAGACTGAAGCTCCTTGAGG187300.15023341925968373No Hit
TGAGGTAGTAGATTGTATAGTC184510.1479955589300814No Hit
TGAGGTAGTAGGTTGTAT184150.1477068027585198No Hit
TTCAAGTAATTCAGGATAGGT175800.141009263779244No Hit
TGAGAACTGAATTCCATGGGTT174380.1398702811025288No Hit
TGAGGGGCAGAGAGCGAGACC168800.13539456044332412No Hit
TTCACAGTGGCTAAGTTCCG161320.12939484887865552No Hit
TGAGGTAGTAGTTTGTGCTGT159740.12812753012569075No Hit
TACCCTGTAGAACCGAATTTGT158390.12704469448233477No Hit
AAGCTGCCAGTTGAAGAACTGC157850.1266115602249924No Hit
TGAGGTAGTAGATTGTAT155850.12500735927187243No Hit
TGAGGTAGTAGTTTGTACAGTT154160.12365180946648609No Hit
TCCTGTACTGAGCTGCCCCA154110.12361170444265808No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTT148810.11936057191689022No Hit
TATTGCACTTGTCCCGGCCTGA147590.11838200933548702No Hit
TGAGGTAGTAGTTTGTGCTGTC146430.11745157278267744No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACA144060.11555059465323031No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCT140470.11267105394237999No Hit
TGAGGTAGTAGATTGTATAGC139980.11227802470886561No Hit
TGGCTCAGTTCAGCAGGAACAG137110.10997599634113846No Hit
TGTAAACATCCTTGACTGGAAGCT136080.1091498328502817No Hit
TCGTACCGTGAGTAATAATGCA135720.10886107667872011No Hit
ACCAATATTACTGTGCTGCTT135590.10875680361676729No Hit
AAACCGTTACCATTACTGAGG128330.10293355415694186No Hit
AAAAGCTGGGTTGAGAGGGCGT127800.10250844090436509No Hit
TGAGGTAGTAGGTTGTGTGGTTT124760.10007005545562275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)