FastQCFastQC Report
Thu 9 May 2024
EGAF00002167638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10029719
Total Bases225.8 Mbp
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT101886610.158470042879566No Hit
TCAGTGCACTACAGAACTTTGT2461032.453737736819945No Hit
TCCTGTACTGAGCTGCCCCGAG2410792.40364660266155No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1548501.5439116489704248No Hit
TCCTGTACTGAGCTGCCCCGA1462081.4577477195522626No Hit
AAACCGTTACCATTACTGAGC1385501.3813946332893274No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1250471.2467647398695816No Hit
TGAGGTAGTAGGTTGTATAGT1204141.200572020013721No Hit
TTCAAGTAATCCAGGATAGGCT1169851.1663836245063297No Hit
CGCGACCTCAGATCAGA1111501.1082065210401209No Hit
TATTGCACTTGTCCCGGCCTGT1070701.0675274152745455No Hit
TGAGGTAGTAGGTTGTATAGTT967830.9649622287523708No Hit
TGAGGTAGTAGGTTGTGTGGT908690.9059974661304071No Hit
CATTGCACTTGTCTCGGTCTGA894850.8921984753511041No Hit
TGAGGTAGTAGATTGTATAGT800910.7985368283996789No Hit
TGAGGTAGTAGGTTGTGTGGTT761990.7597321520174194No Hit
AAACCGTTACCATTACTGAG755360.7531217973305134No Hit
TCCTGTACTGAGCTGCCCCGAGA751510.7492832052423403No Hit
TGAGGTAGTAGTTTGTGCTGTT730770.7286046598115061No Hit
TAGCTTATCAGACTGATGTTGAC726880.7247261862470923No Hit
TGAGGTAGTAGATTGTATAGTT709850.7077466477375887No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT698470.6964003677470924No Hit
TGAGGTAGTAGTTTGTACAGT658990.6570373506974622No Hit
AAGCTGCCAGTTGAAGAACTGT589320.5875737894551183No Hit
TGAGGTAGTAGGTTGTATAG582960.5812326347328375No Hit
TGAGGGGCAGAGAGCGAGACTTT571730.5700359102782441No Hit
TAGCTTATCAGACTGATGTTGA547980.5463562837602928No Hit
TCCTGTACTGAGCTGCCCCG529500.5279310417370616No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC514020.5124969104318875No Hit
TGAGGGGCAGAGAGCGAGACT501870.5003829120237566No Hit
TCGTACCGTGAGTAATAATGCG461230.45986333216314434No Hit
TGGAGTGTGACAATGGTGTTT448860.4475299856356893No Hit
CGCGACCTCAGATCAGACGT447160.44583502289545696No Hit
AAAAGCTGGGTTGAGAGGGCGA417930.4166916341325215No Hit
TGAGGTAGTAGTTTGTACAGTT391630.3904695635042218No Hit
CGCGACCTCAGATC388800.387647949060188No Hit
CCCATAAAGTAGAAAGCACT374900.3737891360665239No Hit
AAACCGTTACCATTACTGAGTT364900.36381876700633387No Hit
TGGCTCAGTTCAGCAGGAAC363220.362143745004222No Hit
TCAGTGCACTACAGAACTTTGC363190.3621138338970414No Hit
TCCTGTACTGAGCTGCCCCGAGT356370.3553140421979918No Hit
TGAGGTAGTAGGTTGTGTGG352850.3518044722888049No Hit
AAACCGTTACCATTACTG336890.33589176326874165No Hit
CATTGCACTTGTCTCGGTCTG333240.33225257856177226No Hit
TTCACAGTGGCTAAGTTCCG312250.3113247739044334No Hit
CTAGACTGAAGCTCCTTGAGG311750.31082625545142395No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC292100.2912344802481505No Hit
TGAGGTAGTAGATTGTATAG282850.28201188886747475No Hit
TAGCTTATCAGACTGATGTTG280090.2792600670068623No Hit
TGAGGTAGTAGTTTGTGCTGT255030.2542743221420261No Hit
TGGCTCAGTTCAGCAGGAACAG254090.25333710745036825No Hit
TGAGAACTGAATTCCATGGGTT252500.251751818769798No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT252390.25164214471013596No Hit
TGTAAACATCCCCGACTGGAAG249080.24834195255121305No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT247210.2464774935369575No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG233640.23294770272227963No Hit
TTCAAGTAATTCAGGATAGGT206510.20589809146198415No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC202100.20150115870644034No Hit
TAGCAGCACGTAAATATTGGCG199750.19915812197729568No Hit
TGTAAACATCCCCGACTGGAAGCT197210.19662564823600742No Hit
TTCAAGTAATCCAGGATAGGCC190150.18958656767951326No Hit
TACAGTACTGTGATAACTGAAG172560.17204868850263902No Hit
AAACCGTTACCATTACTGAGG170130.16962588882101282No Hit
TGAGGTAGTAGGTTGTATAGC169060.1685590593315725No Hit
TATTGCACTTGTCCCGGCCTGC165720.16522895606546903No Hit
TCCTGTACTGAGCTGCCCCGAA165300.16481020056494106No Hit
TGAGATGAAGCACTGTAGCTC161470.16099154921488829No Hit
TGGAGAGAAAGGCAGTTCCTGA159260.15878809765258628No Hit
TGAGGGGCAGAGAGCGAGACTT157070.15660458682840467No Hit
TGTAAACATCCTTGACTGGAAGCT145680.14524833646884824No Hit
CGCGACCTCAGATCAGACG145440.14500904761140368No Hit
AAAAGCTGGGTTGAGAGGGCG143910.1434835811451946No Hit
AAATGGATTTTTGGAGCAGGGAGA142910.14248654423917562No Hit
TATTGCACTTGTCCCGGCCTG141270.14085140371330443No Hit
CAACGGAATCCCAAAAGCAGCTG141000.1405822037486793No Hit
TGAGGTAGTAGTTTGTGCTG139760.13934587798521575No Hit
AGAGGTAGTAGGTTGCATAGT139200.1387875373178451No Hit
TGTAAACATCCCCGACTGGAAGC135570.13516829334899613No Hit
AGAAGCCGGCGGGAGC135230.13482930080094965No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCC130580.1301930791879613No Hit
TGAGGTAGTAGGTTGTAT130150.12976435331837313No Hit
TGAGGTAGTAGGTTGTGTGGC129940.12955497556810913No Hit
CGTGTATTTGACAAGCTGAGTTG128930.12854796829302995No Hit
AGCTCGGTCTGAGGCCCCTCAGT124990.12461964288331508No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC123360.12299447272650411No Hit
TGAGGTAGTAGGTTGTATAGTC122770.12240622095195289No Hit
TGAGATGAAGCACTGTAGCT121450.1210901322360078No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCG121320.12096051743822533No Hit
TAGCAGCACGTAAATATTGGC120240.11988371757972482No Hit
AAACCGTTACCATTACTGAGA119880.11952478429355798No Hit
TGGCTCAGTTCAGCAGGAACA117270.11692251796884838No Hit
CTAGACTGAAGCTCCTTGAGGA112090.11175786679566994No Hit
AGAGGTAGTAGGTTGCATAGTT111660.11132914092608177No Hit
TGAGGTAGTAGATTGTATAGC111320.11099014837803532No Hit
TGGAGAGAAAGGCAGTTCCTG110280.10995322999577556No Hit
AAATGGATTTTTGGAGCAGGGA107520.1072014081351631No Hit
CAACGGAATCCCAAAAGCAGCT106770.10645363045564886No Hit
TCAGTGCATCACAGAACTTTGT105730.1054167120733891No Hit
AGGAAACTCTGGTG105410.10509766026346301No Hit
GCATTGGTGGTTCAGTGGTAGAATT103740.10343260863041129No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT103260.10295403091552216No Hit
TGAGGTAGTAGATTGTAT102570.10226607545036906No Hit
TGTCAGTTTGTCAAATACCCCAA101400.10109954227032682No Hit
AACATTCAACGCTGTCGGTGA101190.10089016452006282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)