FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002167639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002167639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12950679
Sequences flagged as poor quality0
Sequence length14-50
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACCGTTACCATTACTGAGT12603509.731922164081126No Hit
AAACCGTTACCATTACTGAGC2617572.0211835997170495No Hit
TCCTGTACTGAGCTGCCCCGAG2446161.88882760510086No Hit
TCAGTGCACTACAGAACTTTGT2442811.886240868142898No Hit
TGAGGTAGTAGGTTGTATAGT2129871.6446010282549666No Hit
TTCAAGTAATCCAGGATAGGCT2062141.59230261208698No Hit
TGAGGTAGTAGGTTGTGTGGT1657981.2802263109138912No Hit
TGAGGTAGTAGGTTGTATAGTT1634781.262312192279648No Hit
TATTGCACTTGTCCCGGCCTGT1585491.224252411784741No Hit
TCCTGTACTGAGCTGCCCCGA1564031.2076818520480663No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGC1531491.1825557563429685No Hit
TGAGGTAGTAGGTTGTGTGGTT1402821.083201892348656No Hit
AAACCGTTACCATTACTGAGG1328751.0260079799676913No Hit
TGAGGTAGTAGATTGTATAGT1190090.9189402347166509No Hit
TGAGGTAGTAGTTTGTGCTGTT1189890.918785802659459No Hit
TGAGGGGCAGAGAGCGAGACTTT1117060.8625493690330832No Hit
TGGAGTGTGACAATGGTGTTT1111270.8580785609773819No Hit
TGAGGTAGTAGATTGTATAGTT1060920.8192002905793588No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC1036440.8002978067790885No Hit
AAGCTGCCAGTTGAAGAACTGT967420.7470033038422155No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGT933880.721105047851159No Hit
TAGCTTATCAGACTGATGTTGAC844760.6522901231665151No Hit
TGAGGGGCAGAGAGCGAGACT821560.6343760045322719No Hit
TGAGGTAGTAGTTTGTACAGT760420.5871661246487538No Hit
TCCTGTACTGAGCTGCCCCG742590.5733985067501094No Hit
TCCTGTACTGAGCTGCCCCGAGA699940.5404658705539687No Hit
CGCGACCTCAGATCAGA693580.535554931135271No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGC649320.5013791168787366No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACT636040.49112482828120435No Hit
TCCTGTACTGAGCTGCCCCGAGT587520.4536596112064858No Hit
AAACCGTTACCATTACTGAG561560.4336143301829966No Hit
CATTGCACTTGTCTCGGTCTGA546400.4219083802478619No Hit
TCAGTGCACTACAGAACTTTGC539070.41624844535178424No Hit
TCGTACCGTGAGTAATAATGCG523990.40460426823952633No Hit
TTCAAGTAATCCAGGATAGGCC506600.3911764008667036No Hit
CCCATAAAGTAGAAAGCACT500560.38651255273951274No Hit
TGAGGTAGTAGGTTGTGTGG451330.34849910186176336No Hit
TGAGGTAGTAGGTTGTATAGC442990.3420592850768674No Hit
TAGCTTATCAGACTGATGTTGA441940.3412485167766107No Hit
TGAGGTAGTAGGTTGTATAG413690.31943498869827597No Hit
TATTGCACTTGTCCCGGCCTGC411810.31798332736067353No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGT406100.31357429212784904No Hit
AAACCGTTACCATTACTGAGA397450.30689510565430583No Hit
TGGCTCAGTTCAGCAGGAAC388560.30003060071213256No Hit
TGAGGTAGTAGGTTGTGTGGC388280.2998143958320641No Hit
TGAGGTAGTAGGTTGTATAGTC384390.29681069231968454No Hit
TGAGGTAGTAGTTTGTACAGTT346910.26787012480195055No Hit
TCCTGTACTGAGCTGCCCCGAA342250.2642718578693828No Hit
TGTAAACATCCCCGACTGGAAGCT339480.262132973877277No Hit
TGAGGTAGTAGGTTGTGTGGTC337540.260634982922517No Hit
AAACCGTTACCATTACTGAGTT334360.2581795132131682No Hit
TTCAAGTAATCCAGGATAGGCG324480.25055056958789573No Hit
TTCACAGTGGCTAAGTTCCG313590.24214174407380493No Hit
TAGCTTATCAGACTGATGTTG311530.2405510938847299No Hit
AAACCGTTACCATTACTG287980.22236671915040132No Hit
TTCAAGTAATCCAGGATAGGCA279690.21596551037980327No Hit
TGAGAACTGAATTCCATGGGTT278830.21530145253387872No Hit
TCAGTGCACTACAGAACTTTGG273150.210915582109633No Hit
TGAGGTAGTAGTTTGTGCTGTC273010.21080747966959879No Hit
CTAGACTGAAGCTCCTTGAGG271400.2095643016092052No Hit
GGCTGGTCCGAAGGTAGTGAGTTATCTCAAT265900.20531742003643208No Hit
TTCAAGTAATTCAGGATAGGT262960.20304726879571333No Hit
CATTGCACTTGTCTCGGTCTG257550.1988698816486765No Hit
AAAAGCTGGGTTGAGAGGGCGA255670.19741822031107406No Hit
TGAGGTAGTAGTTTGTGCTGT249920.1929782986668112No Hit
CGCGACCTCAGATCAGACGT249510.19266171294956813No Hit
TGAGGTAGTAGATTGTATAGC248940.19222158158657165No Hit
TGTAAACATCCCCGACTGGAAG243920.1883453369510587No Hit
TAGCTTATCAGACTGATGTTGAT236770.18282439090645364No Hit
TGGAGTGTGACAATGGTGTTC233940.18063917729719037No Hit
TGAGGGGCAGAGAGCGAGACTT228460.17640773893013642No Hit
TGAGGTAGTAGGTTGTAT225590.1741916389094348No Hit
AAGCTGCCAGTTGAAGAACTGC219530.16951234757652475No Hit
TGAGGGGCAGAGAGCGAGACTTC213230.16464773777498462No Hit
TGAGGTAGTAGGTTGTATAGG209950.1621150520370399No Hit
TACAGTATAGATGATGTACT204890.15820792099008862No Hit
TATTGCACTTGTCCCGGCCTG203580.15719639101548266No Hit
TGTAAACATCCTTGACTGGAAGCT203030.15677170285820535No Hit
TGGCTCAGTTCAGCAGGAACAG198800.15350546484859984No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCG198520.15328925996853138No Hit
TATTGCACTTGTCCCGGCCTGG198020.152903179825552No Hit
TGAGGGGCAGAGAGCGAGACC196370.15162911535372006No Hit
TCCTGTACTGAGCTGCCCCA195780.15117354078500442No Hit
TGAGGTAGTAGATTGTATAGTC193920.14973732265312112No Hit
AGCTCGGTCTGAGGCCCCTCAGT188380.14545955466890964No Hit
TGAGGTAGTAGATTGTATAG187630.14488043445444057No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGA184670.14259484000800265No Hit
CAACGGAATCCCAAAAGCAGCT184520.14247901596510887No Hit
CAACGGAATCCCAAAAGCAGCTG182390.1408343145560167No Hit
TGAGGTAGTAGGTTGTGTGGG180740.13956025008418477No Hit
TGAGGTAGTAGATTGTAT180590.13944442604129095No Hit
TCCTGTACTGAGCTGCCCCGG173020.13359917267658322No Hit
GCATTGGTGGTTCAGTGGTAGAATT172860.13347562703082982No Hit
AAGCTGCCAGTTGAAGAACT171440.13237915942476838No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAT167010.1289584893579711No Hit
AGAGGTAGTAGGTTGCATAGT161890.1250050286938623No Hit
TGAGGTAGTAGTTTGTACAGC160100.12362286178199615No Hit
TACAGTACTGTGATAACTGAAG157820.12186233633001019No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACC152490.11774672200585004No Hit
GCATTTGTGGTTCAGTGGTAGAATTCTCGC152380.11766178437439459No Hit
TCCTGTACTGAGCTGCCCCGAGG150480.11619467983107294No Hit
AGAGGTAGTAGGTTGCATAGTT147240.11369288050456659No Hit
TAGCAGCACGTAAATATTGGCG146110.1128203393814332No Hit
TCGTACCGTGAGTAATAATGCA143350.11068917699218704No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTC133790.10330732465842138No Hit
TCAGTGCATCACAGAACTTTGT133740.10326871664412345No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)